Highly magnetic Fe2O3 nanoparticles synthesized by laser pyrolysis used for biological and heat transfer applications

Dumitrache, Florian; Morjan, I.; Fleaca, C.; Badoi, A.; Manda, Gina; Pop, Sevinci; Marta, Daciana; Huminic, Gabriela; Huminic, Angel; Vékás, L.; Daia, Camelia; Marinică, Oana; Luculescu, C.; Niculescu, A.

doi:10.1016/j.apsusc.2014.12.098

Cited by 33 publications

(19 citation statements)

References 23 publications

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“…We utilized iron oxide NPs to take advantage of the magnetic attraction technology [19], which introduces NPs into cells by magnetism [17,20]. We designed NPs with iron oxide to introduce more NPs and used PLGA and PEI to make it easier for NPs to bind to the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

“…The surface modi cation of NPs can affect the interaction with cellular molecules. Several studies have reported a link between cytotoxicity and intracellular capacity [20,23]. The absorption process is important in the physicochemical properties of the core particles and the coating.…”

Section: Endocytic Mechanism Of Plga-pei Pcs Nps On Mscsmentioning

confidence: 99%

“…NPs encapsulated with PLGA and polyethyleneimine (PEI) are approximately 100 nm in size and are used for drug delivery and clinical applications [15,18,19]. Altering the surface charge of NPs has demonstrated that positively charged NPs are more readily introduced into cells than are negatively charged NPs [16,20]. The reason for using magnetic iron oxide NPs in this study was to introduce more NPs into cells using magnetic force.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of stem cell-derived exosome release efficiency by surface modified nanoparticles

Yun

Kim

Park

et al. 2020

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Background: Mesenchymal stem cells (MSCs) are pluripotent stromal cells that release extracellular vesicles (EVs). EVs contain various growth factors and antioxidants that can positively affect the surrounding cells. Nanoscale MSC-derived EVs, such as exosomes, have been developed as bio-stable nano-type materials. However, some issues such as low yield and difficulty in quantification limit their use. We hypothesized that enhancing exosome production using nanoparticles would stimulate intracellular molecules. Results: Our aim in this study was to elucidate the molecular mechanisms of exosome generation by comparing the internalization of surface-modified, positively charged nanoparticles and exosome generation from MSCs. We determined that Rab7, which is located in the multiple vesicle body and autolysosomal membrane, was increased upon exosome expression and was associated with autophagosome formation. Conclusions: Nanoparticles may migrate to lysosomes during treatment. Simultaneously, intracellular exosome-forming factors can be stimulated during endosomal maturation. MSC-derived exosomes using nanoparticles may increase exosome yield and enable the discovery of nanoparticle-induced genetic factors.

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Section: Discussionmentioning

confidence: 99%

Section: Endocytic Mechanism Of Plga-pei Pcs Nps On Mscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improvement of stem cell-derived exosome release efficiency by surface modified nanoparticles

Yun

Kim

Park

et al. 2020

Preprint

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“…The preparation of stabilized suspensions was done according the same procedure as in [ 27 ] when l -DOPA was used as stabilizer. As a particularity, Fe-Si nanoaggregates showed a good stability in distilled water (pH ~5.5, due to the atmospheric CO 2 absorption) but their magnetic properties decreased down to 60% in comparison with those dispersed in acetone (a base fluid presumed to be inert, which does not modify the particles magnetic properties).…”

Section: Methodsmentioning

confidence: 99%

“…Several studies have focused on the design of new generation of nanoparticle systems with biocompatibility, enhanced dispersibility, internalization, and targeting capabilities improved by surface modifications [ 15 , 19 ]. In this respect, various biocompatible agents, such as polyethylene glycol (PEG)-derived compounds [ 20 , 21 ], alginate [ 22 , 23 ], chitosan [ 24 ], dextran [ 25 ], heparin [ 26 ], l -3,4-dihydroxyphenylalanine ( l -DOPA) [ 27 , 28 ], phosphonates or carboxymethyldextran [ 29 ], Na carboxymethyl-cellulose (CMC-Na) [ 30 ] were used for NPs coatings. Many of those cited polymeric coating agents or other synthetic polymers, as well as the well-known biological molecules—proteins, peptides, lipids, or nucleic acids—can also form various nano-assemblies [ 31 ], without the introduction of an inorganic core.…”

Section: Introductionmentioning

confidence: 99%

Coating Dependent In Vitro Biocompatibility of New Fe-Si Nanoparticles

et al. 2018

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Magnetic nanoparticles offer multiple utilization possibilities in biomedicine. In this context, the interaction with cellular structures and their biological effects need to be understood and controlled for clinical safety. New magnetic nanoparticles containing metallic/carbidic iron and elemental silicon phases were synthesized by laser pyrolysis using Fe(CO)5 vapors and SiH4 gas as Fe and Si precursors, then passivated and coated with biocompatible agents, such as l-3,4-dihydroxyphenylalanine (l-DOPA) and sodium carboxymethyl cellulose (CMC-Na). The resulting magnetic nanoparticles were characterized by XRD, EDS, and TEM techniques. To evaluate their biocompatibility, doses ranging from 0–200 µg/mL hybrid Fe-Si nanoparticles were exposed to Caco2 cells for 24 and 72 h. Doses below 50 μg/mL of both l-DOPA and CMC-Na-coated Fe-Si nanoparticles induced no significant changes of cellular viability or membrane integrity. The cellular internalization of nanoparticles was dependent on their dispersion in culture medium and caused some changes of F-actin filaments organization after 72 h. However, reactive oxygen species were generated after exposure to 25 and 50 μg/mL of both Fe-Si nanoparticles types, inducing the increase of intracellular glutathione level and activation of transcription factor Nrf2. At nanoparticles doses below 50 μg/mL, Caco2 cells were able to counteract the oxidative stress by activating the cellular protection mechanisms. We concluded that in vitro biological responses to coated hybrid Fe-Si nanoparticles depended on particle synthesis conditions, surface coating, doses and incubation time.

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