Highly Galloylated Tannin Fractions from Witch Hazel (<i>Hamamelis virginiana</i>) Bark: Electron Transfer Capacity, In Vitro Antioxidant Activity, and Effects on Skin-Related Cells

Touriño, Sonia; Lizarraga, Daneida; Carreras, Anna; Lorenzo, S. Aixut; Ugartondo, Vanessa; Mitjans, Montserrat; Vinardell, M. Pilar; Juliá, Luís; Cascante, Marta; Torres, Josep Lluı́s

doi:10.1021/tx700425n

Cited by 68 publications

(90 citation statements)

References 59 publications

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“…In addition to these systems, a balanced diet containing basic nutrients as well as antioxidant-rich foods may have an important role in maintaining lower levels of ROS (Touriño et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant comparative effects of two grape pomace Mexican extracts from vineyards on erythrocytes

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Antioxidant comparative effects of two grape pomace Mexican extracts from vineyards on erythrocytes

et al. 2016

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“…Due to their antiphlogistic and astringent properties, these extracts are widely used in skin care, to treat small wounds, local inflammations [39]–[41], or hemorrhoids [42]. In addition, antimutagenic as well as antioxidant properties have been described [43]–[45].…”

Section: Introductionmentioning

confidence: 99%

Tannins from Hamamelis virginiana Bark Extract: Characterization and Improvement of the Antiviral Efficacy against Influenza A Virus and Human Papillomavirus

et al. 2014

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Antiviral activity has been demonstrated for different tannin-rich plant extracts. Since tannins of different classes and molecular weights are often found together in plant extracts and may differ in their antiviral activity, we have compared the effect against influenza A virus (IAV) of Hamamelis virginiana L. bark extract, fractions enriched in tannins of different molecular weights and individual tannins of defined structures, including pseudotannins. We demonstrate antiviral activity of the bark extract against different IAV strains, including the recently emerged H7N9, and show for the first time that a tannin-rich extract inhibits human papillomavirus (HPV) type 16 infection. As the best performing antiviral candidate, we identified a highly potent fraction against both IAV and HPV, enriched in high molecular weight condensed tannins by ultrafiltration, a simple, reproducible and easily upscalable method. This ultrafiltration concentrate and the bark extract inhibited early and, to a minor extent, later steps in the IAV life cycle and tannin-dependently inhibited HPV attachment. We observed interesting mechanistic differences between tannin structures: High molecular weight tannin containing extracts and tannic acid (1702 g/mol) inhibited both IAV receptor binding and neuraminidase activity. In contrast, low molecular weight compounds (<500 g/mol) such as gallic acid, epigallocatechin gallate or hamamelitannin inhibited neuraminidase but not hemagglutination. Average molecular weight of the compounds seemed to positively correlate with receptor binding (but not neuraminidase) inhibition. In general, neuraminidase inhibition seemed to contribute little to the antiviral activity. Importantly, antiviral use of the ultrafiltration fraction enriched in high molecular weight condensed tannins and, to a lesser extent, the unfractionated bark extract was preferable over individual isolated compounds. These results are of interest for developing and improving plant-based antivirals.

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“…Witch hazel plants contain secondary compounds, including those widely known for their medicinal qualities, such as polyphenols, that have clear antioxidant properties (i.e., as free radical scavengers) (Tourino et al, 2008). Therefore, differences between the leachate chemistry of witch hazel and other leaf types likely exist but were not detected by our broad chemical measurements.…”

Section: Discussionmentioning

confidence: 90%

Effect of single-species and mixed-species leaf leachate on bacterial communities in biofilms

Blackwood

Leff

2009

Hydrobiologia

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Dissolved organic matter in the form of leaf leachate represents an important carbon and energy source in many lotic ecosystems. In this study, we investigated utilization of mono-specific and mixed-species leaf leachate and impacts on biofilm bacterial community structure. Ceramic tiles were incubated in a Northeast Ohio stream to allow for biofilm development and then exposed in the laboratory to glucose or leachate from: sugar maple (Acer saccharum), pin oak (Quercus palustris), maple ? oak, American beech (Fagus grandifolia), witch hazel (Hamamelis virginiana), or beech ? witch hazel. Bacterial responses to these amendments were compared to un-amended controls based on fluorescent in situ hybridization (FISH) targeting selected taxa and terminal restriction fragment length polymorphism (T-RFLP) of bacterial 16S rRNA genes; also changes in DOC concentrations were quantified. Generally, there were limited differences among communities as a result of leachate amendment, although specific taxa monitored by FISH exhibited differential responses. There was no evidence that mixing of leachate from different leaf species created an effect different than what could be expected based on monospecific experiments. Witch hazel solicited the greatest response, based on T-RFLP data, regardless of whether the community was exposed to witch hazel alone or witch hazel ? beech, accounting for 19% of the variability in Jaccard distance (P \ 0.05) and 27% of the variability in Hellinger distance among profiles. In conclusion, we found that leaf leachate can be readily degraded but only in some cases did differences in leaf leachate among tree species cause an alteration in community structure. Mixing of leachate from different leaf species did have an impact on DOC loss but did not alter community structure. The occurrence of particular compounds, such as those in witch hazel, may alter community structure suggesting that the presence and abundance of specific plant taxa can impact bacterial communities.

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Highly Galloylated Tannin Fractions from Witch Hazel (Hamamelis virginiana) Bark: Electron Transfer Capacity, In Vitro Antioxidant Activity, and Effects on Skin-Related Cells

Cited by 68 publications

References 59 publications

Antioxidant comparative effects of two grape pomace Mexican extracts from vineyards on erythrocytes

Antioxidant comparative effects of two grape pomace Mexican extracts from vineyards on erythrocytes

Tannins from Hamamelis virginiana Bark Extract: Characterization and Improvement of the Antiviral Efficacy against Influenza A Virus and Human Papillomavirus

Effect of single-species and mixed-species leaf leachate on bacterial communities in biofilms

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