Highly efficient system of plant regeneration from protoplasts of grapevine (Vitis vinifera L.) through somatic embryogenesis by using embryogenic callus culture and activated charcoal

Zhu, Yanming; Hoshino, Yoichiro; Nakano, Masaru; Takahashi, E.; Mii, Masahiro

doi:10.1016/s0168-9452(96)04557-8

Cited by 55 publications

(34 citation statements)

References 28 publications

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“…root regeneration can also be induced de novo from various mature somatic tissues, and whole plants can be regenerated even from single protoplasts through de novo organogenesis or somatic embryogenesis (Takebe et al, 1971;Zhu et al, 1997;Chupeau et al, 2013) (Fig. 3).…”

Section: Diverse Forms Of Regeneration In Seed Plantsmentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

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Compared with animals, plants generally possess a high degree of developmental plasticity and display various types of tissue or organ regeneration. This regenerative capacity can be enhanced by exogenously supplied plant hormones in vitro, wherein the balance between auxin and cytokinin determines the developmental fate of regenerating organs. Accumulating evidence suggests that some forms of plant regeneration involve reprogramming of differentiated somatic cells, whereas others are induced through the activation of relatively undifferentiated cells in somatic tissues. We summarize the current understanding of how plants control various types of regeneration and discuss how developmental and environmental constraints influence these regulatory mechanisms.

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Section: Diverse Forms Of Regeneration In Seed Plantsmentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

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“…For four gentian species studied in this work, a greater percentage of dividing cells was obtained when protoplasts were cultured on medium containing TDZ than when grown on medium supplemented with BAP. Browning of the protoplast cultures of G. cruciata and G. septemfida, which was observed within 2 weeks from culture initiation, is thought to be caused by oxidation of phenolic compounds released from plant cells into the culture medium (Zhu et al 1997). This phenomenon was also described by Takahata and Jomori (1989); Jomori et al (1995); Kunitake et al (1995) and Nakano et al (1995), and indicates that it is one of the major problems in protoplast cultures of Gentianaceae.…”

Section: Discussionmentioning

confidence: 93%

“…In the case of G. cruciata and G. septemfida, weekly medium refreshment was insufficient to prevent cell browning. Whether the addition of activated charcoal or polyvinylpyrrolidone (PVP) is beneficial for these species, as in Eustoma grandiflorum or Vitis vinifera (Zhu et al 1997), would be worth investigating. Another possibly valuable modification would be conditioned (Chen et al 2004;Komai et al 2006) or nurse type of protoplast culture (Zhou et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the morphogenic potential of five Gentiana species in leaf mesophyll protoplast culture and ploidy stability of regenerated calli and plants

Tomiczak

Śliwińska

Rybczyński

2016

Plant Cell Tiss Organ Cult

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The morphogenic potential of five Gentiana species of medicinal and ornamental value, i.e.G. cruciata, G. kurroo, G. lutea, G. septemfida, and G. tibetica, was compared using agarose-bead leaf mesophyll protoplast culture. Modified Murashige and Skoog medium containing 2.0 mg l -1 1-naphthaleneacetic acid and 0.1 mg l -1 thidiazuron ensured the highest cell division frequency during both protoplast and protoplast-derived cell culture or callus formation. Indirect plant regeneration mainly via the somatic embryogenesis pathway was achieved for G. kurroo and G. tibetica with the greatest efficiency occurring with MS medium supplemented with 1.0 mg l -1 kinetin, 0.5 mg l -1 gibberellic acid, and 80 mg l -1 adenine sulfate. A considerable percentage of autopolyploid and aneuploid cells was detected in callus lines obtained from protoplasts using flow cytometry. The presence of polyploid cells in calli resulted in the regeneration of 85 % polyploid plants of G. kurroo and 14 % polyploids of G. tibetica. Chromosome counting and stomatal characteristic confirmed the ploidy variation among regenerants.

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“…However, the methods involving the use of hydrolytic enzymes have been the most popular ones [106]. The protoplasts used for transformation are usually isolated by enzymatic digestion of mesophyll cells from leaves [107][108][109], berry mesocarp [106,110], roots [111], stems [112], embryogenic tissue [108,113,114], and from fast-grown suspension-cultured cells [115][116][117].…”

Section: Polyethylene Glycol (Peg) Treatment and Electroporation Of Pmentioning

confidence: 99%

“…protoplasts, diminishing its viability [118,119]. Despite this, some progress has been reached in obtaining whole plants from protoplast regeneration [113,114]. However, plant protoplasts constitute a versatile system for transient gene expression and have been widely used for the functional characterization of genes [109], virus inoculation [107,108], protein subcellular localization [109,117], promoter analysis [115], protein-DNA interaction [116], and protein-protein interaction [109].…”

Section: Polyethylene Glycol (Peg) Treatment and Electroporation Of Pmentioning

confidence: 99%

Development and Use of Biotechnology Tools for Grape Functional Analysis

Chialva¹,

Eichler²,

Muñoz³

et al. 2016

Grape and Wine Biotechnology

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The aim of this chapter is to provide a description of the latest scientific advances in the field of gene functional analysis in grapevine. It provides general information about the studies conducted during the past decade to understand the natural variation of this plant and how this information has been exploited for the understanding of traits of interest. Likewise, it is exposed how the use of biotechnology tools have helped to characterize the mechanisms of gene expression and its regulation, as well as the subcellular localization of proteins and their interactions with other molecules. Finally, an approximation to the new technologies of gene editing and their potential application in the functional study of grapevine has been carried out.

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Highly efficient system of plant regeneration from protoplasts of grapevine (Vitis vinifera L.) through somatic embryogenesis by using embryogenic callus culture and activated charcoal

Cited by 55 publications

References 28 publications

Plant regeneration: cellular origins and molecular mechanisms

Plant regeneration: cellular origins and molecular mechanisms

Comparison of the morphogenic potential of five Gentiana species in leaf mesophyll protoplast culture and ploidy stability of regenerated calli and plants

Development and Use of Biotechnology Tools for Grape Functional Analysis

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