Highly Efficient Synthesis and Assay of Protein‐Imprinted Nanogels by Using Magnetic Templates

Mahajan, Rashmi; Rouhi, Mona; Shinde, Sudhirkumar; Bedwell, Thomas S.; İncel, Anıl; Mavliutova, Liliia; Piletsky, Sergey A.; Nicholls, Ian A.; Sellergren, Börje

doi:10.1002/ange.201805772

Cited by 11 publications

(12 citation statements)

References 20 publications

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“…There are several studies focusing on imprinting of small molecules [ 40 ], peptides [ 41 ] and proteins [ 42 , 43 ], however, there is still a great challenge about imprinting of microorganisms [ 22 ]. The three-dimensional shape, size and complex surface chemistry of the microorganisms contribute to reduce the sensitivity when using SPR for quantifying microorganisms.…”

Section: Resultsmentioning

confidence: 99%

Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

et al. 2021

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Over the past few decades, a significant increase in multi-drug-resistant pathogenic microorganisms has been of great concern and directed the research subject to the challenges that the distribution of resistance genes represent. Globally, high levels of multi-drug resistance represent a significant health threat and there is a growing requirement of rapid, accurate, real-time detection which plays a key role in tracking of measures for the infections caused by these bacterial strains. It is also important to reduce transfer of resistance genes to new organisms. The, World Health Organization has informed that millions of deaths have been reported each year recently. To detect the resistant organisms traditional detection approaches face limitations, therefore, newly developed technologies are needed that are suitable to be used in large-scale applications. In the present study, the aim was to design a surface plasmon resonance (SPR) sensor with micro-contact imprinted sensor chips for the detection of Staphylococcus aureus. Whole cell imprinting was performed by N-methacryloyl-L-histidine methyl ester (MAH) under UV polymerization. Sensing experiments were done within a concentration range of 1.0 × 102–2.0 × 105 CFU/mL. The recognition of S. aureus was accomplished by the involvement of microcontact imprinting and optical sensor technology with a detection limit of 1.5 × 103 CFU/mL. Selectivity of the generated sensor was evaluated through injections of competing bacterial strains. The responses for the different strains were compared to that of S. aureus. Besides, real experiments were performed with milk samples spiked with S. aureus and it was demonstrated that the prepared sensor platform was applicable for real samples.

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Section: Resultsmentioning

confidence: 99%

Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

et al. 2021

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“…[ 160 ] This strategy is beneficial for macromolecule templates that experience mass‐transfer hindrance. [ 161 ] The generated imprinted cavities are 2D rather than 3D, since only a portion of the templates are imprinted while the other templates do not need to penetrate inside the polymer framework to reach recognition sites. Under these conditions, the polymerization time is significantly reduced by several minutes to a few hours compared with nonoriented surface imprinting.…”

Section: Green Strategies For Benign Mipsmentioning

confidence: 99%

“…Accordingly, by replacing glass beads with magnetic particles as the solid support and by developing new synthetic approaches, this obstacle was largely overcome (25 mg g −1 magnetic particles compared to 0.4 mg g −1 glass beads). [ 161a,174 ]…”

Section: Green Strategies For Benign Mipsmentioning

confidence: 99%

Molecular Imprinting: Green Perspectives and Strategies

et al. 2021

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Large quantities of organic solvents and reagents are usually required in the fabrication of these materials. Moreover, the use of excess reagents in solvent extraction, purification, filtration/ centrifugation, and cleaning processes is essential for adequate product purity. Because of the numerous practical applications of advanced materials, some are synthesized on a large scale and commercialized. [3] The excessive consumption of nonrenewable, hazardous chemicals is an unsustainable practice and a prominent criticism of these materials. Nonetheless, the use of advanced materials in sensing applications, medicine, electronics, and catalysis is inevitable. [4] During various research activities, such as fabricating advanced materials, as well as in the subsequent stage, i.e., industrial operations, new challenges have arisen that impact not only almost every aspect of human life but also other living creatures and ecosystems. It is estimated that the impact of these practices will endure into the next century. [5] Since the advancement of science, industry, and technology is inevitable, scientists have been persuaded to search for methods to counteract or minimize the negative impacts of human activities. [6] Thus, green chemistry has advanced and green principles have been defined for most sciences, including synthesis, [7] analytical chemistry, [8] engineering, [9] and pharmaceutical science. [10] Fortunately, contemporary society has great awareness of deleterious environmental side effects, resulting in a sense Advances in revolutionary technologies pose new challenges for human life; in response to them, global responsibility is pushing modern technologies toward greener pathways. Molecular imprinting technology (MIT) is a multidisciplinary mimic technology simulating the specific binding principle of enzymes to substrates or antigens to antibodies; along with its rapid progress and wide applications, MIT faces the challenge of complying with green sustainable development requirements. With the identification of environmental risks associated with unsustainable MIT, a new aspect of MIT, termed green MIT, has emerged and developed. However, so far, no clear definition has been provided to appraise green MIT. Herein, the implementation process of green chemistry in MIT is demonstrated and a mnemonic device in the form of an acronym, GREENIFICATION, is proposed to present the green MIT principles. The entire greenificated imprinting process is surveyed, including element choice, polymerization implementation, energy input, imprinting strategies, waste treatment, and recovery, as well as the impacts of these processes on operator health and the environment. Moreover, assistance of upgraded instrumentation in deploying greener goals is considered. Finally, future perspectives are presented to provide a more complete picture of the greenificated MIT road map and to pave the way for further development.

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“…The developed MINA allows to avoid the interference of other compounds, such as mercaptoethanol and sugars, that are well-known to affect traditional avidin and streptavidin-based assays. MINA has also been applied to the detection of the insecticide methyl parathion (MP) [ 146 ], proteins [ 147 ], leukotrienes and insulin [ 148 ] in biological samples. In particular, the developed MINA test for the detection of methyl parathion exhibited a high specificity and a linearity in a wide concentration range similar to ELISA.…”

Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 99%

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

Parisi

Francomano

Dattilo

et al. 2022

JFB

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Molecular recognition is a useful property shared by various molecules, such as antibodies, aptamers and molecularly imprinted polymers (MIPs). It allows these molecules to be potentially involved in many applications including biological and pharmaceutical research, diagnostics, theranostics, therapy and drug delivery. Antibodies, naturally produced by plasma cells, have been exploited for this purpose, but they present noticeable drawbacks, above all production cost and time. Therefore, several research studies for similar applications have been carried out about MIPs and the main studies are reported in this review. MIPs, indeed, are more versatile and cost-effective than conventional antibodies, but the lack of toxicity studies and their scarce use for practical applications, make it that further investigations on this kind of molecules need to be conducted.

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Highly Efficient Synthesis and Assay of Protein‐Imprinted Nanogels by Using Magnetic Templates

Cited by 11 publications

References 20 publications

Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

Molecular Imprinting: Green Perspectives and Strategies

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

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