Whole Cell Recognition of Staphylococcus aureus Using Biomimetic SPR Sensors

Abstract

Over the past few decades, a significant increase in multi-drug-resistant pathogenic microorganisms has been of great concern and directed the research subject to the challenges that the distribution of resistance genes represent. Globally, high levels of multi-drug resistance represent a significant health threat and there is a growing requirement of rapid, accurate, real-time detection which plays a key role in tracking of measures for the infections caused by these bacterial strains. It is also important to reduce transfer of resistance genes to new organisms. The, World Health Organization has informed that millions of deaths have been reported each year recently. To detect the resistant organisms traditional detection approaches face limitations, therefore, newly developed technologies are needed that are suitable to be used in large-scale applications. In the present study, the aim was to design a surface plasmon resonance (SPR) sensor with micro-contact imprinted sensor chips for the detection of Staphylococcus aureus. Whole cell imprinting was performed by N-methacryloyl-L-histidine methyl ester (MAH) under UV polymerization. Sensing experiments were done within a concentration range of 1.0 × 102–2.0 × 105 CFU/mL. The recognition of S. aureus was accomplished by the involvement of microcontact imprinting and optical sensor technology with a detection limit of 1.5 × 103 CFU/mL. Selectivity of the generated sensor was evaluated through injections of competing bacterial strains. The responses for the different strains were compared to that of S. aureus. Besides, real experiments were performed with milk samples spiked with S. aureus and it was demonstrated that the prepared sensor platform was applicable for real samples.

Nanoarchitectonic E-Tongue of Electrospun Zein/Curcumin Carbon Dots for Detecting Staphylococcus aureus in Milk

“…Indeed, the limits of detection (LoD) were 1.74, 4.19, and 0.83 CFU/mL for the three sensing units, namely, cCDOT/ZEI, cCDOT, and ZEI, respectively, with no false positives. These values are competitive with the devices in the literature [8][9][10][11][12][13][14][15][16][17]54,56 with the advantage of having sensing units that do not require specific interactions with a given analyte. This reduces fabrication costs, especially compared to biosensors.…”

Advancements in microfluidics for skin cosmetic screening

“…The principle is to recognize Staphylococcus aureus through micro-contact imprinting and optical sensor technology. 65…”

Terminally Phosphorylated Triblock Polyethers Acting Both as Templates and Pore-Forming Agents for Surface Molecular Imprinting of Monoliths Targeting Phosphopeptides

“…Therefore, other approaches, namely, oriented surface imprinting, epitope imprinting, and dual template docking oriented molecular imprinting (DTD-OMI) have been developed. In oriented surface printing, the removal of the template will result in specific binding sites only on the polymer surface, , providing better access for the target molecule than bulk imprinting. − In epitope printing, only a small portion or fragment of the target molecule (an epitope) is used as the template to create an imprint for the target, , whereby more specific and strong interactions can be obtained. This can create imprints that recognize templates, peptides, or even whole proteins .…”