Highly efficient plant regeneration and Agrobacterium-mediated transformation of Helianthus tuberosus L.

Kim, Mee Jin; An, Dong; Moon, Ki Beom; Cho, Hye Sun; Min, Sung Ran; Sohn, Jae Hak; Jeon, Jae Heung; Kim, Hyun Soon

doi:10.1016/j.indcrop.2015.12.054

Cited by 22 publications

(24 citation statements)

References 51 publications

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“…Any results of transformations from previous research indicated that infection period varied from few minutes to few hours, 5 min on Artemisia carvifolia [5]; 30 min on Oncidium Gower Ramsey, Crambe abyssinica , and Dendrobium chrysotoxum Lindl [42], [43], [44]; 40 min on Cordyline fruticosa [45]; an hour on Helianthus tuberosus [46]; 4 h on Erycina pusilla [10]. The results of observation (Fig.…”

Section: Discussionmentioning

confidence: 82%

Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: An important medicinal orchid

Utami

Hariyanto

Manuhara

2018

Journal of Genetic Engineering and Biotechnology

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A protocol for genetic transformation mediated by Agrobacterium tumefaciens and production of transgenic Dendrobium lasianthera has been developed for the first time. The 8-week-old protocorm explants were used as target of transformation with Agrobacterium tumefaciens strain LBA4404 carrying plasmid pG35SKNAT1. Several parameters such as infection period, Agrobacterium density, concentration of acetosyringone, and co-cultivation period were evaluated for the transformation efficiency. The data were analyzed using one-way analysis of variance (ANOVA) and Duncan's Multiple Range Test (DMRT) with p < 0.05. Subsequently, KNAT1 gene expression was confirmed by polymerase chain reaction (PCR) analysis. The highest efficiency of transformation (70%) obtained from protocorm explants infected with Agrobacterium culture was at the OD 600 concentration of 0.6 for 30 min, and co-cultivated with acetosyringone 100 µM for 5 days. The results of confirmation by PCR analysis show that the KNAT1 gene has been integrated and expressed in the genome of Dendrobium lasianthera transgenic.

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Section: Discussionmentioning

confidence: 82%

Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: An important medicinal orchid

Utami

Hariyanto

Manuhara

2018

Journal of Genetic Engineering and Biotechnology

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“…In general, cytokinin alone or a high cytokinin-to-low auxin ratio was found to be necessary for the induction of callus and adventitious shoots in plant regeneration. Among the plant growth hormones, cytokinin such as 6-BA combined with auxin NAA have been widely used for callus induction and shoot proliferation in various plants (Kim et al 2016;Kwapata et al 2010;Zhang et al 2017). In our previous study, we have established in vitro adventitious shoot induction protocol (5 mg l −1 6-BA +0.05 mg l −1 NAA) for N. cadamba using cotyledon as explant.…”

Section: The Hormonal Controls Behind Plant Regenerationmentioning

confidence: 99%

High frequency plant regeneration from leaf culture of <i>Neolamarckia cadamba</i>

Deng

Ouyang

et al. 2019

Plant Biotechnology

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Neolamarckia cadamba is a miracle tree species with considerable economic potential uses as a timber wood, woody forage and traditional medicine resource. The present study aimed to establish a highly efficient and robust protocol of plant regeneration for N. cadamba. Greenish callus was induced from very young leaf explants of sterile in vitro plantlets cultured on Murashige and Skoog's (MS) medium supplemented with 3 mg l −1 thidiazuron (TDZ), 0.1 mg l −1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.05 mg l −1 α-naphthaleneacetic acid (NAA). The callus could differentiate into nodular embryogenic structures or adventitious shoots, and these two regeneration pathways often occurred in the same callus clumps. The micro-shoots developed roots in MS supplemented with 0.05 mg l −1 NAA and 0.05 mg l −1 indole-3butyric acid (IBA), while the nodular embryogenic structures germinated directly and developed into plantlets on induction medium contained with 0.5 mg l −1 (or 1 mg l −1) 6-benzyladenine (6-BA) and 0.05 mg l −1 NAA. The rooted plantlets could be successfully acclimatized to a greenhouse with more than 92.0% survival. This regeneration protocol can be used in large scale cultivation needs and may be useful for future genetic modifications of N. cadamba.

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“…The Ht1-SST and Ht1-FFT genes were introduced in potato plants via Agrobacterium-mediated transformation [27]. The gene isolation, vector construction, plant growth, and plant transformation methodologies are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Expression of Jerusalem artichoke (Helianthus tuberosus L.) fructosyltransferases, and high fructan accumulation in potato tubers

Moon

Park

et al. 2019

Appl Biol Chem

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Fructans are polymers of fructose that are present as storage carbohydrates in various plants. Jerusalem artichoke (Helianthus tuberosus L.) contains a high amount of inulin. Two enzymes are involved in inulin biosynthesis. The sucrose:sucrose 1-fructosyltransferase (1-SST) enzyme mainly catalyzes the synthesis of 1-kestose from sucrose. In the next step, fructan:fructan 1-fructosyltransferase (1-FFT) catalyzes the synthesis of inulin from 1-kestose. In this study, the Ht1-SST and Ht1-FFT genes were isolated from Jerusalem artichoke and expressed in potato (Solanum tuberosum L.), either separately or together, via Agrobacterium-mediated transformation. Transgenic potato tubers overexpressing Ht1-SST accumulated 1-kestose to a high level (up to 3.36 mg/g), while tubers overexpressing both Ht1-SST and Ht1-FFT accumulated up to 3.14 mg/g short-chain inulin-type fructans, with the degree of polymerization (DP) ranging from 3 to 5, excluding high DP inulins. Transgenic potato plants accumulated fructo-oligosaccharides to a high level, following the fructan biosynthetic pathway of Jerusalem artichoke, and therefore present a high potential for the mass production of inulin through established potato breeding and cultivation methods.

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Highly efficient plant regeneration and Agrobacterium-mediated transformation of Helianthus tuberosus L.

Cited by 22 publications

References 51 publications

Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: An important medicinal orchid

Agrobacterium tumefaciens-mediated transformation of Dendrobium lasianthera J.J.Sm: An important medicinal orchid

High frequency plant regeneration from leaf culture of <i>Neolamarckia cadamba</i>

Expression of Jerusalem artichoke (Helianthus tuberosus L.) fructosyltransferases, and high fructan accumulation in potato tubers

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