Highly efficient microfluidic sorting device for synchronizing developmental stages of C. elegans based on deflecting electrotaxis

Wang, Xixian; Hu, Rui; Ge, Anle; Hu, Liang; Wang, Shanshan; Feng, Xiaojun; Du, Wei; Liu, Bi‐Feng

doi:10.1039/c5lc00354g

Cited by 45 publications

(31 citation statements)

References 39 publications

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“…Currently, several dilution micro-devices have been proposed for generating logarithmic, liner, and parabolic chemical concentration gradients [28][29][30][31][32][33][34][35][36]. Compared to traditional methods, microfluidic devices could generate linear chemical concentration gradient which shown great potential in studying chemotaxis behavior [22,[37][38][39], screening of chemotaxis-defective mutants [23,40] and drug screening in C. elegans [20,37,41]. Although these "Christmas tree" mixers had been well established and applied to create concentration gradients, they are not suitable for generating wide-range concentration gradient, such as 1000 fold dilution.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, microfluidic device has been recognized as a new platform for fundamental studies in C. elegans because of microchannel's comparable dimension to the tiny worms and the versatile use of micro-flow. "Worm-chip" have successfully used in phenotyping and stage screening, nerve system imaging, behavioral dynamics, microsurgery and microinjection [18][19][20][21][22][23][24][25][26][27]. Currently, several dilution micro-devices have been proposed for generating logarithmic, liner, and parabolic chemical concentration gradients [28][29][30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

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Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Wang

et al. 2018

Sensors and Actuators B: Chemical

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a b s t r a c t C. elegans is a commonly used model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradientcustomizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 10 8 cells mLand further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Wang

et al. 2018

Sensors and Actuators B: Chemical

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“…Microfluidic systems have been developed for cultivation , sorting , behaviour studies , and imaging studies in C. elegans . Using electrotaxis, C. elegans has also been effectively synchronized, sorted, and immobilized in microfluidic systems as an alternative to conventional large‐scale systems . The electrotaxis of C. elegans has mostly been analysed at the individual worm level.…”

Section: Introductionmentioning

confidence: 99%

Effectively controlled microfluidic trap for spatiotemporal analysis of the electrotaxis of Caenorhabditis elegans

et al. 2018

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C. elegans is a popular model organism with a well-developed neural network. Approximately 60% of the genes in C. elegans have genomic counterparts in humans, including those involved in building neural circuits. Therefore, we can extend the study of human neural network mechanisms to C. elegans which is easy to genetically manipulate. C. elegans shows behavioural responses to various external physical and chemical stimuli. Electrotaxis is one of its distinct behavioural responses, which is defined as movement towards the cathode in an electric field. In this study, we developed an effective microfluidic trap system for analysing electrotaxis in C. elegans. In addition, two mutant strains (unc-54(s74) and unc-6(e78)) from wild-type (N2) worms were screened using the system. Wild-type (N2) worms and the two mutant strains clearly showed different behavioural responses to the applied electric field, thus enabling the effective screening of the mutant worms from the wild type (N2). This microfluidic system can be utilized as a platform for the study of behavioural responses, and for the sorting and mutant screening of C. elegans.

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“…Thus microfluidic systems are used as a platform for various studies with C. elegans. [20][21][22][23][24][25][26][27][28] McCormick et al demonstrated a microfluidic device for analysis of C. elegans orientation. 29 They designed the device in order to immobilize C. elegans and only allow the movement of the head of C. elegans.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Platform for Analyzing the Thermotaxis of C. elegans in a Linear Temperature Gradient

et al. 2017

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Caenorhabditis elegans (C. elegans), which shares a considerable amount of characteristics with human genes is one of the important model organisms for the study of behavioral responses. Thermotaxis is a representative behavior response of C. elegans; C. elegans stores the cultivation temperature in thermosensory neurons and moves to the cultivation temperature region in a temperature variation. In this study, we developed a microfluidic system for effective thermotaxis analysis of C. elegans. The microfluidic channel was fabricated using polydimethylsiloxane (PDMS) by soft lithography process. The temperature gradient (15 -20 C) was generated in the microchannel and controlled by Peltier modules attached to the bottom of the channel. The thermotaxis of wild type (N2), tax-4(p678) and ttx-7(nj50) mutants were effectively analyzed using this microfluidic system. We believe that this system can be employed as a basic platform for studying the neural circuit of C. elegans responding to external stimuli.

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Highly efficient microfluidic sorting device for synchronizing developmental stages of C. elegans based on deflecting electrotaxis

Abstract: We propose a PDMS–agarose hybrid microfluidic chip for simultaneous sorting of all the different stages of C. elegans.

Cited by 45 publications

References 39 publications

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Effectively controlled microfluidic trap for spatiotemporal analysis of the electrotaxis of Caenorhabditis elegans

Microfluidic Platform for Analyzing the Thermotaxis of C. elegans in a Linear Temperature Gradient

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