Highly efficient in vitro regeneration of the industrial oilseed crop Crambe abyssinica

Li, Xueyuan; Ahlman, Annelie; Lindgren, Helén; Zhu, Lu

doi:10.1016/j.indcrop.2010.10.004

Cited by 26 publications

(35 citation statements)

References 24 publications

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“…O óleo de sementes de crambe contém 55-60% de ácido erúcico, que faz o óleo não-comestível (Li et al, 2011), podendo ser usado como matéria-prima para a produção de biocombustiveis ocorrendo, recentemente, relatos de cultivo comercial em pequena escala em estados do centrooeste, como Mato Grosso do Sul, com bom potencial de rendimento -2000 kg ha -1 (Pitol, 2008). O estudo do processo de secagem fornece informações relativas ao comportamento do fenômeno de transferência de calor e massa entre o material biológico e o elemento de secagem, normalmente ar atmosférico, aquecido ou não; essas informações são fundamentais para o projeto, operação e simulação de sistemas de secagem e secadores (Corrêa et al, 2003).…”

Section: Introductionunclassified

Cinética de secagem de sementes de crambe

Faria¹,

Teixeira²,

Devilla

et al. 2012

Rev. bras. eng. agríc. ambient.

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R ESU M OO estudo da cinética de secagem é de fundamental importância para a modelagem matemática, a qual tem sido muito usada no projeto e em análise dos processos de transferência de calor e de massa, durante a secagem. Buscou-se, então, avaliar a capacidade de ajuste de nove modelos matemáticos comumente utilizados para descrever o processo de secagem em sementes de crambe (Crambe abyssinica Horts), através de um delineamento inteiramente casualizado com três repetições, em que os tratamentos são constituídos de cinco teores de água (0,11; 0,13; 0,17; 0,25 e 0,28 decimal b.s) e cinco temperaturas do ar de secagem (30; 40; 50; 60 e 70 ºC). Para verificação do ajuste dos modelos estudados fez-se análise de regressão não-linear, pelo método Quasi-Newton. De acordo com os dados obtidos concluise que: o modelo da Aproximação por Difusão é o que melhor se ajusta aos dados de secagem obtidos; o aumento da temperatura de secagem promove maior taxa de remoção da água do produto; o coeficiente de difusão apresenta valores da ordem de 0,18 × 10 -10 e 3,92 × 10 -10 m 2 s -1 e a energia de ativação para a difusão líquida foi da ordem de 4,97 kJ mol Drying kinetics of crambe seedsA B ST R A C T Drying kinetics is fundamental for mathematical and modeling widely used in the project and in the analysis of heat and mass transfer during drying. In this study it was sought to assess the capability of adjustment of nine mathematical models commonly used to describe the seed drying process of Crambe abyssinica Horts. The experimental design was completely randomized, with three replications. The treatments were five water contents (0.11; 0.13; 0.17; 0.25 and 0.28 decimal dry weight) and five drying temperatures (30; 40; 50; 60 and 70 ºC). To determine the adjustment of the studied models a non linear regression analyses was performed according to Quasi-Newton method. According to the data obtained it was concluded that: the diffusion approximation method was the model that best adjusted to the drying data obtained; increases in drying temperature promoted higher rate of moisture removal from the seeds; the diffusion coefficient showed values of 0.19 × 10 -10 and 3.9 × 10 -10 m 2 s -1; the activation energy for liquid diffusion during drying of Crambe abyssinica Horts was about 4.97 kJ mol -1 .

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Section: Introductionunclassified

Cinética de secagem de sementes de crambe

Faria¹,

Teixeira²,

Devilla

et al. 2012

Rev. bras. eng. agríc. ambient.

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“…Of note, this was the time point when signs of a decline in NSE were observed during treatment with 5.0 µM BAP after 90 days. A positive effect of BAP on the regeneration of a wide range of explants has been previously reported by Li et al (2011), when 10 µM thidiazuron (TDZ) and 2.5 µM ANA were used and 95% shoot regeneration was reported in hypocotyls of the C. abyssinica cultivar Galactica. These results demonstrate that the balance between cytokinins and auxins is important, and that it affects the morphogenetic response in vitro.…”

Section: Resultsmentioning

confidence: 67%

“…In this context, in vitro cell and tissue culturing techniques are important both for mass propagation Li et al, 2011;Palmer and Keller, 2011;Chhikara et al, 2012) and as a tool for genetic improvement (Wang et al, 2004;Wang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Micropropagation studies have documented crambe regeneration in vitro via direct Li et al, 2011) and indirect organogenesis (Chhikara et al, 2012), using hypocotyls as explants for both, and via indirect somatic embryogenesis (Palmer and Keller, 2011) with cotyledons, hypocotyls, and roots as explants. However, no published data describe crambe regeneration using apical segments via multiplication by axillary bud proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…Crambe tissue culturing has been used to propagate (Li et al, 2011;Palmer and Keller, 2011), genetically improve Chhikara et al, 2012;Li et al, 2013), and to identify and use some of its genes to improve other species (Wang et al, 2004;Wang et al, 2006). The use of molecular markers in crambe reported in the literature is limited to the study of genetic diversity (Lara-Fioreze et al, 2013), identification and characterization of genetic transformants (Wang et al, 2004;Wang et al, 2006;Li et al, 2010;Xu et al, 2010;Chhikara et al, 2012;Li et al, 2013), and identification of genes of interest (Paulose et al, 2010;Cheng et al, 2013).…”

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confidence: 99%

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Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner¹,

Soares²,

Gontijo³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar 'FMS brilhante' using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers. The aim was to develop a protocol for the in vitro regeneration of these plants with low genetic variation as compared to the donor plant. For micropropagation, shoot tips from in vitro germinated seedlings were used as explants and were initially cultivated for 90 days on MS medium with 5.0 µM 6-benzylaminopurine (BAP), which at 90 days, led to the highest number of shoots per explant (NSE) (12.20 shoots) being detected. After 120 days, the interaction between BAP concentration and naphthalene acetic acid (NAA) was tested, and the highest NSE was observed following exposure to 0.0/0.5 µM BAP/NAA (11.40 shoots) and 1.0/0.0 µM BAP/NAA (11.00 shoots). The highest proportion of rooting phase were observed following exposure to 0.5 µM NAA (30%). The 13 ISSR primers used to analyze genetic stability produced 91 amplification products, of which only eight bands were polymorphic and 83 were monomorphic for all 10 regenerated crambe plants, compared to the donor plant explant. These results indicate that crambe shoot tips are a highly reliable explant that can be used to micropropagate genetically true-to-type plants or to maintain genetic stability, as verified using ISSR markers.

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Neglected Oil Crop Biotechnology

Tiwari

Kumar

2013

Biotechnology of Neglected and Underutilized Crops

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Highly efficient in vitro regeneration of the industrial oilseed crop Crambe abyssinica

Cited by 26 publications

References 24 publications

Cinética de secagem de sementes de crambe

Cinética de secagem de sementes de crambe

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Neglected Oil Crop Biotechnology

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