Highly efficient in-line wet cyclone air sampler for airborne virus detection

Different Detection Methods of Blood Nucleic Acid and Enzyme-linked Immunosorbent Assay for Blood Virus Screening

“…In amplification technology, a pair of primers is added together with a special fluorescent probe. The probe is an oligonucleotide, a report fluorescent group and a quenched fluorescent group are labeled on both sides of the fluorescent probe (19)(20). When the probe is intact, the quenching group absorbs the fluorescence signal emitted by the reporting group, and the fluorescence signal cannot be detected; at the beginning of amplification, the probe binds to a corresponding single strand of DNA; during PCR amplification, Taq has used The 5 '-3' end exonuclease of the enzyme cleaves and degrades the probe, separating the reported fluorescence group and the quenched fluorescence group on the probe, so that the fluorescence monitoring system can receive the fluorescence signal, that is, when amplifying a DNA chain, a new fluorescence molecular signal is detected, realizing the synchronous formation process of PCR product and the accumulation of fluorescence signal (21)(22).…”

Recent Advances on Bioaerosol Collection and Detection in Microfluidic Chips

“…To determine the distribution of bioaerosols containing pathogenic microorganisms, the bioaerosols are often collected from the air for subsequent pathogen detection. So far, some traditional bioaerosol collection methods, such as sedimentation, filtration, centrifugation, impaction, and impingement, have been reported to collect and concentrate the bioaerosols. − To the best of our knowledge, there are still no standard methods and procedures for collection of bioaerosols containing pathogenic microorganisms in many countries. However, it is essential to accurately detect the bioaerosols for early prediction and real-time warning of airborne diseases.…”

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

“…The centrifugation method is also reported to collect the bioaerosols. In general, the bioaerosols are injected into a special-structured chamber to form the swirling air, resulting in the centrifugation of the bioaerosols into the collection wall or liquid due to their different mass [ 41 , 42 , 43 , 44 , 45 ]. A typical example was reported by Jung et al and shown in Figure 3 .…”

“…The immunological detection methods are based on the antibody-antigen reaction. In the past decades, various biosensors for detection of the pathogens in the bioaerosols have received increasing attention due to their outstanding features of low cost, fast response, miniature size, and easy integration, and have been considered as a promising candidate for in-field applications in many fields, such as biomedical diagnostics, food safety, environmental monitoring, and so on [ 45 , 103 , 104 ].…”