Highly efficientin vitroproliferation and genetic stability analysis of micropropagatedCeropegiaevansiiby RAPD and ISSR markers: A critically endangered plant of Western Ghats

Chavan, Jaykumar J.; Gaikwad, Nikhil B.; Kshirsagar, Parthraj R.; Umdale, Suraj D.; Bhat, K. V.; Dixit, G. B.; Yadav, S. R.

doi:10.1080/11263504.2013.853700

Cited by 40 publications

(19 citation statements)

References 28 publications

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“…Therefore, the genetic uniformity assessment of regenerated plants is of great importance. In recent years, random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) have successfully used for assessing the genetic fidelity of regenerated plantlets in many plant species [16][17][18] .…”

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confidence: 99%

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Cui

Deng

Zheng

et al. 2019

Sci Rep

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Magnolia sirindhorniae Noot. & Chalermglin is an endangered species with high ornamental and commercial value that needs to be urgently protected and judiciously commercialized. In this study, a protocol for efficient regeneration of this species is standardized. The lateral buds of the M. sirindhorniae plant were used as an explant. Half-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 6-benzyladenine (BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 2.0 mg/L gibberellic acid (GA 3 ) was found to be the optimal medium for shoot induction. The maximum shoot multiplication rate (310%) was obtained on Douglas-fir cotyledon revised medium (DCR) fortified with 0.2 mg/L BA, 0.01 mg/L NAA, and additives. The half-strength DCR medium supplemented with 0.5 mg/L NAA and 0.5 mg/L indole-3-butyric acid (IBA) supported the maximum rate (85.0%) of in vitro root induction. After a simple acclimatization process, the survival rate of plantlets in a substrate mixture of sterile perlite and peat soil (1:3; v/v ) was 90.2%. DNA markers were used for assessment of genetic uniformity, confirming the genetic uniformity and stability of regenerated plants of M. sirindhorniae . Thus, the described protocol can safely be applied for large scale propagation of this imperative plant.

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confidence: 99%

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Cui

Deng

Zheng

et al. 2019

Sci Rep

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“…Raw material can be produced by plant tissue culture studies and use for the food, drug, and cosmetic industries [16]. In the investigations carried out by Sing et al [8], Asthana et al [18] and Chavan et al [19] no genetic differences were identified during the RAPD analyses applied to micropropagated plants. In the study, shoot tips were used as explants, and no callus formation was observed during the performance of micropropagation.…”

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confidence: 99%

Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Çetin

2018

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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Determination of the genetic characters of the plants obtained using plant tissue culture methods is important. In this study, the genetic fidelity of the plants, which are obtained by the micropropagation of the shoot tip explants of the Origanum majorana L. plant of medicinal and economical value in Murashige and Skoog (MS) medium which contains 1.0 mg/L Benzil Amino Purin (BAP), 0.1 mg/L naphthaleneacetic acid (NAA), 3% sucrose and 0.7% agar, has been investigated by using random amplified polymorphic DNA (RAPD-PCR) technique. Monomorphic bands were obtained as a result of all of the RAPD-PCR analyses performed. According to the results obtained, no polymorphism was detected among the micropropagated plants.

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“…Many factors can influence the stability of plants during tissue culture [ 45 ]. Therefore, it is important to evaluate the genetic stability of regenerated plants [ 46 , 47 , 48 ]. In the present study, RAPD and ISSR markers were used to analyze the genetic stability of regenerated plants after two years of subculture.…”

Section: Discussionmentioning

confidence: 99%

Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants

Kang

Zheng

Xie

et al. 2020

Plants

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Magnolia lucida (Magnoliaceae) is classified as an endangered species by the International Union for Conservation of Nature. It has high commercial value owing to its attractive tree shape and flowers. We adopted an excellent genotype of M. lucida as the parent material and established a mini-cut orchard through grafting to provide trunk shoots explants over the long-term. Optimal sterilization was achieved using a combination of 75% ethanol for 30 s, one percent benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog medium (ML) was the optimal medium for the growth of M. lucida. Addition of one mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of α-naphthaleneacetic acid (NAA) to the medium increased the shoot induction rate to 95.56%, and the ML medium containing 0.4 mg/L BA and 0.04 mg/L NAA achieved the maximum multiplication rate (284.56%). Dark treatment for seven days, followed by continuous light treatment could better resolve the challenge of difficult rooting in M. lucida plants. Using random amplified polymorphic DNA and inter simple sequence repeat markers, we confirmed the genetic uniformity and stability of the regenerated plants. Our protocol should be helpful for the propagation and conservation of this endangered plant.

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Highly efficientin vitroproliferation and genetic stability analysis of micropropagatedCeropegiaevansiiby RAPD and ISSR markers: A critically endangered plant of Western Ghats

Cited by 40 publications

References 28 publications

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants

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