Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Çetin, Burcu

doi:10.18466/cbayarfbe.406873

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Several research articles have already been published on the detection of genetic fidelity using RAPD markers in different crops such as Acacia mangium [74], Simmondsia chinensis [75], Ocimum gratissimum [76] Stevia rebaudiana [77], Origanum majorana [78] and Salvia hispanica [79]. It is worth mentioning that RAPD analysis is an easy-access technique for different laboratories but, usually, the reproducibility of the results are not satisfactory [80][81][82][83].…”

Section: Genetic Uniformity Assessment Based On Rapd and Scot Markersmentioning

confidence: 99%

Establishment of an Efficient Micropropagation System for Humulus lupulus L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers

Clapa

Hârța

2021

Agronomy

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The demand for virus-free hop planting material has increased in the last few years due to its multipurpose uses. The present study aimed to establish an effective protocol for clonal propagation of cv. Cascade using only the cytokinins as PGRs in all stages of micropropagation: (i) in vitro culture initiation using single-node micro-cuttings inoculated on modified Murashige and Skoog (MSm) medium solidified with Plant agar and supplemented with 0.5 mg L−1 6-benziyladenine (BA) with 76% recorded viability of nodal explants; (ii) in vitro multiplication of multinodal shoots on MSm medium gelled with Plant agar and supplemented with different types and concentrations of cytokinins: 2 mg L−1 kinetin (KIN), 0.7 mg L−1 1-(2-Chloro-4-pyridyl)-3-phenylurea) (1 CPPU), 2 mg L−1 meta-topoline (mT) and 0.5 mg L−1 BA, which was the best variant for shoot proliferation (9.48 ± 0.78 shoots/explant); (iii) rooting and acclimatization with the best results obtained by ex vitro rooting and acclimatization of plants in the same stage in perlite (96.00 ± 0.60% acclimatized rooted plants with 100% survival under greenhouse conditions). The true-to-type nature of in vitro raised plants with the mother plant was assessed by Random Amplified Polymorphic DNA (RAPD) and Start Codon Target Polymorphism (SCoT) molecular markers, and then their genetic uniformity were confirmed.

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Section: Genetic Uniformity Assessment Based On Rapd and Scot Markersmentioning

confidence: 99%

Establishment of an Efficient Micropropagation System for Humulus lupulus L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers

Clapa

Hârța

2021

Agronomy

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“…However, newly developed microplants grown from the meristems of the in vitro propagated shoots may show genetic variability (Sliwinska, Thiem, 2007). So, the plants grown in vitro require assessment of their genetic sta-bility using the ploidy level and/or DNA content analysis (Sliwinska, Thiem, 2007;Fritsche et al, 2022b;Catalano et al, 2023), molecular markers (Çetin, 2018;Bulavin et al, 2021), or both methods (Abdolinejad et al, 2020;Raji, Farajpour, 2021).…”

Section: Introductionmentioning

confidence: 99%

Anatomy, ploidy level, and essential oil composition of Hyssopus officinalis ʻNikitskiy Beliyʼ in vitro and ex situ

Bulavin,

Ivanova,

Miroshnichenko

et al. 2024

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Clonal micropropagation is a biotechnological method for plant multiplication. The existing data on the structure of organs in vitro, genetic stability, and essential oil composition are limited for Hyssopus officinalis L., so this study was aimead at investigating these aspects under a short period of in vitro culturing.Materials and methods. Plants of Hyssopus officinalis ʻNikitskiy Beliyʼ cultivated ex situ, in vitro and ex vitro were analyzed. Conventional methods were applied to study plant anatomy, ploidy level, and relative DNA content, as well as to extract and analyze essential oil. Statistical analysis was performed using the Past 4.03 software.Results. According to the results obtained, with 6-BAP introduced into MS nutrient medium in optimal concentrations (0.3– 0.5 mg/L), the general in vitro structure of leaf blades in the developed microshoots was similar to those in ex situ plants, while the qualitative and quantitative changes observed were induced by the effect of specific culturing conditions and plant rejuvenation. The analysis of the ploidy level and relative DNA content in the nuclei isolated from the leaf tissue cells of the microshoots ex vitro after adaptation revealed no changes compared to the ex situ leaf parameters. The mass fraction of essential oil and its component composition in the mother plants and ex vitro regenerants were similar.Conclusion. Cultivation of Hyssopus officinalis ʻNikitskiy Beliyʼ microshoots on MS nutrient medium with 6-BAP optimal concentrations promotes morphogenesis without significant deviations in the ploidy level, relative DNA content, essential oil yield, or its component composition. The developed protocol for clonal micropropagation of Hyssopus officinalis ʻNikitskiy Beliyʼ provides clones identical to the ex situ plants.

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Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Cited by 2 publications

References 15 publications

Establishment of an Efficient Micropropagation System for Humulus lupulus L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers

Establishment of an Efficient Micropropagation System for Humulus lupulus L. cv. Cascade and Confirmation of Genetic Uniformity of the Regenerated Plants through DNA Markers

Anatomy, ploidy level, and essential oil composition of <i>Hyssopus officinalis</i> ʻNikitskiy Beliyʼ <i>in vitro</i> and <i>ex situ</i>

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