Highly Efficient Förster Resonance Energy Transfer in a Fast, Serum‐Compatible Immunoassay

Kreisig, Thomas; Hoffmann, Ralf; Züchner, Thole

doi:10.1002/cbic.201300073

Cited by 6 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whilst solution-based fluorescent sensors offer a significant advantage in terms of bindingkinetics over analogous heterogenous sensors. [31] Heterogenous immobilisation of a fluorescent sensor is preferential as it avoids contamination of the sensor in a practical situtation, i.e. in vivo.…”

Section: Resultsmentioning

confidence: 99%

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

Xu¹,

Sedgwick²,

Elfecky³

et al. 2018

Preprint

View full text Add to dashboard Cite

A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection. In solution, the fluorescent probe displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M).

show abstract

Section: Resultsmentioning

confidence: 99%

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

Xu¹,

Sedgwick²,

Elfecky³

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Thus, as a consequence of minimizing jitter, the needed transfer rate decreases with the maximum number of photons to be resolved. One mechanism for energy transfer is Forster resonant energy transfer (FRET), with time scales on the order of picoseconds 30 and efficiencies that can attain near 100%, 31 which can be further tuned with the optical environment. 32 This implies that the configuration of Figure 4 would be able to resolve photon number as long as the pulse is longer than a picosecond.…”

Section: ■ Physical Realization and Parametersmentioning

confidence: 99%

Design of High-Performance Photon-Number-Resolving Photodetectors Based on Coherently Interacting Nanoscale Elements

2020

View full text Add to dashboard Cite

A number of applications in basic science and technology would benefit from high fidelity photon number resolving photodetectors. While some recent experimental progress has been made in this direction, the requirements for true photon number resolution are stringent, and no design currently exists that achieves this goal. Here we employ techniques from fundamental quantum optics to demonstrate that detectors composed of subwavelength elements interacting collectively with the photon field can achieve high performance photon number resolution. We propose a new design that simultaneously achieves photon number resolution, high efficiency, low jitter, low dark counts, and high count rate. We discuss specific systems that satisfy the design requirements, pointing to the important role of nanoscale device elements. arXiv:1909.07911v1 [quant-ph]

show abstract

“…Since FRET occurs over distances at biologically relevant length scales (∼1−20 nm), many biological phenomena that produce changes in molecular proximity (e.g., ligand−receptor binding, molecular structural changes) can be investigated using this technique. In this context, homogeneous FRET assays have been widely used for sensitive detection of molecules such as drugs, 72 antigens, 62,73 proteins, 74 peptides, 75 and cancer biomarkers. 76 Despite the simplicity of homogeneous FRET detection, background fluorescence interferences caused by molecules present in the complex biological fluids may mask the detection signal.…”

Section: Detection In Solutionmentioning

confidence: 99%

Fluorescence Sensing of Circulating Diagnostic Biomarkers Using Molecular Probes and Nanoparticles

Tagit

Hildebrandt

2016

ACS Sens.

View full text Add to dashboard Cite

The interplay of photonics, nanotechnology, and biochemistry has significantly improved the identification and characterization of multiple types of biomarkers by optical biosensors. Great achievements in fluorescence-based technologies have been realized, for example, by the advancement of multiplexing techniques or the introduction of nanoparticles to biochemical and clinical research. This review presents a concise overview of recent advances in fluorescence sensing techniques for the detection of circulating disease biomarkers. Detection principles of representative approaches, including fluorescence detection using molecular fluorophores, quantum dots, and metallic and silica nanoparticles, are explained and illustrated by pertinent examples from the recent literature. Advanced detection technologies and material development play a major role in modern biosensing and consistently provide significant improvements toward robust, sensitive, and versatile platforms for early detection of circulating diagnostic biomarkers.

show abstract

Highly Efficient Förster Resonance Energy Transfer in a Fast, Serum‐Compatible Immunoassay

Cited by 6 publications

References 26 publications

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

Design of High-Performance Photon-Number-Resolving Photodetectors Based on Coherently Interacting Nanoscale Elements

Fluorescence Sensing of Circulating Diagnostic Biomarkers Using Molecular Probes and Nanoparticles

Contact Info

Product

Resources

About