Highly efficient exosome purification from human plasma by tangential flow filtration based microfluidic chip

Han, Zhenzhen; Peng, Cheng; Yi, Jia; Zhang, Dongxue; Xiang, Xiaowei; Peng, Xinyan; Su, Bin; Liu, Baohong; Shen, Yuhui; Qiao, Liang

doi:10.1016/j.snb.2021.129563

Cited by 61 publications

(48 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study by Han et al, 114 it was reported that the purity and recovery rate of EVs isolated using tangential flow microfluidics were superior to microfluidic methods based on the dead-end filtration. They fabricated a microfluidic platform integrating a polycarbonate filter (pore size of 100 nm) sandwiched between two poly(methyl methacrylate) plates with serpentine microchannels (15 cm length, 500 μm width, 80 μm depth, and 500 μm interval) to isolate and purify exosomes by tangential flow filtration (flow rate of 50 μL/ min).…”

Section: Label-free Microfluidic Methods For Exosome Isolationmentioning

confidence: 99%

Label-Free Isolation of Exosomes Using Microfluidic Technologies

2021

View full text Add to dashboard Cite

Exosomes are cell-derived structures packaged with lipids, proteins, and nucleic acids. They exist in diverse bodily fluids and are involved in physiological and pathological processes. Although their potential for clinical application as diagnostic and therapeutic tools has been revealed, a huge bottleneck impeding the development of applications in the rapidly burgeoning field of exosome research is an inability to efficiently isolate pure exosomes from other unwanted components present in bodily fluids. To date, several approaches have been proposed and investigated for exosome separation, with the leading candidate being microfluidic technology due to its relative simplicity, costeffectiveness, precise and fast processing at the microscale, and amenability to automation. Notably, avoiding the need for exosome labeling represents a significant advance in terms of process simplicity, time, and cost as well as protecting the biological activities of exosomes. Despite the exciting progress in microfluidic strategies for exosome isolation and the countless benefits of label-free approaches for clinical applications, current microfluidic platforms for isolation of exosomes are still facing a series of problems and challenges that prevent their use for clinical sample processing. This review focuses on the recent microfluidic platforms developed for labelfree isolation of exosomes including those based on sieving, deterministic lateral displacement, field flow, and pinched flow fractionation as well as viscoelastic, acoustic, inertial, electrical, and centrifugal forces. Further, we discuss advantages and disadvantages of these strategies with highlights of current challenges and outlook of label-free microfluidics toward the clinical utility of exosomes.

show abstract

Section: Label-free Microfluidic Methods For Exosome Isolationmentioning

confidence: 99%

Label-Free Isolation of Exosomes Using Microfluidic Technologies

2021

View full text Add to dashboard Cite

show abstract

“…As a result, tangential flow filtration (TFF) strategy, a method originally used for extracting viruses and proteins, was adapted to prevent the filtrated particles from damage, possibly by allowing the dissolved substances to tangentially filtrate through the membrane. During the TFF, samples can be re-circulated to repeat the process several times to improve the recovery of exosomes (Haraszti et al, 2018;Busatto et al, 2018;Han et al, 2021). Given the advantage of high sample capacity and automation, and the ability to isolate exosomes with high purity, it is possible to produce stem cell-derived exosomes on an industrial scale by using TFF.…”

Section: Size-based Techniquementioning

confidence: 99%

“…By this means, the extracting device can precisely isolate the exosomes based on their physical and chemical properties. Other isolation strategies, such as tangential flow filtration (Han et al, 2021), can be integrated with the microfluidic chip and greatly improve the efficiency and specificity of exosome extraction, in particular for smallsized samples (Liu et al, 2017;Contreras-Naranjo et al, 2017).…”

Section: Microfluidicsmentioning

confidence: 99%

Techniques for increasing the yield of stem cell-derived exosomes: what factors may be involved?

Feng

Zhang

Tan

et al. 2021

Sci. China Life Sci.

View full text Add to dashboard Cite

Exosomes are nano-scale extracellular vesicles secreted by cells and constitute an important part in the cell-cell communication. The main contents of the exosomes include proteins, microRNAs, and lipids. The mechanism and safety of stem cell-derived exosomes have rendered them a promising therapeutic strategy for regenerative medicine. Nevertheless, limited yield has restrained full explication of their functions and clinical applications To address this, various attempts have been made to explore the up- and down-stream manipulations in a bid to increase the production of exosomes. This review has recapitulated factors which may influence the yield of stem cell-derived exosomes, including selection and culture of stem cells, isolation and preservation of the exosomes, and development of artificial exosomes.

show abstract

“…Among various cell stretching techniques [ 23 ], the microfluidic technology is of many advantages, such as low reagent consumption, precise control, fast analysis, and integration of multiple functions [ 24 ]. The microfluidic technology has been applied in many fields, such as biosensing [ 25 , 26 ], organs-on-a-chip [ 27 , 28 ], droplet microfluidics [ 29 , 30 ], and microfiltration [ 31 , 32 ]. Microfluidic chips made of polydimethylsiloxane (PDMS) are particularly suitable for cell culture and real-time observation because of the transparency and gas permeability of the PDMS matrix.…”

Section: Introductionmentioning

confidence: 99%

Design and aligner-assisted fast fabrication of a microfluidic platform for quasi-3D cell studies on an elastic polymer

Yang

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

Highly efficient exosome purification from human plasma by tangential flow filtration based microfluidic chip

Cited by 61 publications

References 40 publications

Label-Free Isolation of Exosomes Using Microfluidic Technologies

Label-Free Isolation of Exosomes Using Microfluidic Technologies

Techniques for increasing the yield of stem cell-derived exosomes: what factors may be involved?

Design and aligner-assisted fast fabrication of a microfluidic platform for quasi-3D cell studies on an elastic polymer

Contact Info

Product

Resources

About