Highly Efficient Doubled-Haploid Production in Wheat ( L.) via Induced Microspore Embryogenesis

Liu, Weiguo; Zheng, M. Y.; Polle, E.; Konzak, C. F.

doi:10.2135/cropsci2002.0686

Cited by 58 publications

(43 citation statements)

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“…During androgenesis of Triticum aestivum (Bothma and Duberry 1991), Nicotiana tabacum, and Datura innoxia (Villanueva et al 1985), put and spd were found to be the major PAs, and could be relevant in anther culture. Recent studies with wheat have also shown genetic variability in androgenic traits with respect to embryo induction and plant regeneration (Liu et al 2002;Zheng 2003).…”

Section: Discussionmentioning

confidence: 99%

Effects of exogenous application of polyamines on wheat anther cultures

Redha

Suleman

2010

Plant Cell Tiss Organ Cult

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Androgenesis of wheat genotypes was evaluated by pretreating anthers or embryo-like structures (ELS) with polyamines. Anthers of the genotype DH were pretreated with different concentrations of putrescine, spermidine, and spermine for 1, 3, and 6 h, and those of drought-tolerant International Center for Agricultural Research in the Dry Areas (ICARDA) wheat accessions were treated for 1 and 3 h. ELS of two genotypes were also treated for 30 and 60 min with the same polyamines and evaluated for green plant regeneration. The pretreatment of anthers with polyamines enhanced the development of ELS in all genotypes. The formation of ELS varied significantly with genotype. Pretreated anthers showed that four treatments improved significantly green plant regeneration with the genotype ICR 17. However, two treatments (1 mM putrescine or spermine for 1 h) significantly improved green plant regeneration per 100 ELS of only two ICAR-DA genotypes. ELS treated with polyamines for 30 min were greener and formed more adventitious roots. The chloroplasts of these greener ELS examined with a transmission electron microscope had agranal to grana thylakoids, while those of the control had plastids with mostly starch globules. Although exogenous application of polyamines to anthers improved the production of ELS and green plants, the effects of putrescine, spermidine, and spermine was dependent on genotype and the duration of pretreatment of anthers with the polyamines.

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Section: Discussionmentioning

confidence: 99%

Effects of exogenous application of polyamines on wheat anther cultures

Redha

Suleman

2010

Plant Cell Tiss Organ Cult

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“…The use of DH plants can also increase the selection efficiency because fewer plants are necessary for the screening of selected traits, which reduces costs, allowing even in vitro selection of specific traits. Liu et al (2002) used in vitro selection in studies of resistance to pathogens, mineral imbalances, and drought tolerance. Moreover, this tool is also used in basic and advanced research (Germanà, 2011), such as genetic analysis, induction of mutation, genome mapping, gene transfer (Abdollahi et al, 2007), and QTL detection (Seymour et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Poersch-Bortolon

Scagliusi

Lau

et al. 2016

Pesq. agropec. bras.

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-The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced more embryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.Index terms: Triticum aestivum, albinism, androgenesis, doubled haploid, isolated microspore culture, recalcitrance. Resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas e a um agente gelificanteResumo -O objetivo deste trabalho foi analisar a resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas, antes da cultura de micrósporos isolados, e ao efeito de um agente gelificante no meio de cultura de indução. Cinco genótipos foram avaliados quanto à formação de embriões, regeneração de plantas verdes e à duplicação espontânea dos cromossomos. Espigas de trigo foram submetidas a dois pré-tratamentos: frio, a 4°C por 21 dias; e ácido 2-hidroxinicotínico, a 32°C por dois dias. Os meios de cultura foram avaliados com ou sem Ficoll como agente gelificante. O frio produziu mais embriões e plantas verdes do que o pré-tratamento químico, em quatro dos cinco genótipos testados. Apenas dois genótipos tratados com ácido 2-hidroxinicotínico foram capazes de produzir plantas, e um deles produziu uma única planta albina. O meio com Ficoll produziu mais embriões do que o meio líquido e gerou maior número de plantas. A duplicação espontânea dos cromossomos varia entre os genótipos e os pré-tratamentos e apresenta alta variabilidade.Termos para indexação: Triticum aestivum, albinismo, androgênese, duplo-haploide, cultura de micrósporos isolados, recalcitrância.

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“…Efforts toward improving microspore culture have been made on "stress" treatments such as starvation, heat shock, ethanol stress, water stress, anaerobic conditions, low temperature treatment to induce microspore/pollen embryogenesis by switching the preprogrammed gametophytic to sporophytic development pathway [62][63][64][65][66]. Other methods such as irradiation, treatment with cochicine and auxin can also be applied to induce microspore embryogenesis [38,61], for example, about 50% of treated microspores were successfully redirected for the predicted pathway by a chemical inducer formulation in wheat [67]. However, genotype dependency strongly limited the wide application of microspore/pollen embryogenesis [68].…”

Section: Concluding Remarks and Future Challengesmentioning

confidence: 99%

Haploid Strategies for Functional Validation of Plant Genes

Shen

Pan

Lübberstedt

2015

Trends in Biotechnology

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Increasing knowledge of plant genome sequences requires the development of more reliable and efficient genetic approaches for genotype-phenotype validation. Functional identification of plant genes is generally achieved by a combination of creating genetic modifications and observing the according phenotype, which begins with forward-genetic methods represented by random physical and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing. A major bottleneck is time need to produce modified homozygous genotypes that can actually be used for phenotypic validation. Herein, we comprehensively address and compare available experimental approaches for functional validation of plant genes, and propose haploid strategies to reduce the time needed and cost consumed for establishing gene function. Classic and current approaches to establish plant gene functionThe classic approach for plant gene function establishment began with loss-of-function mutagenesis after treatment with mutagens such as radiation with X-rays or neutrons, or chemicals that introduce random small deletions or point mutations in plant genomes [5,8]. Chemical mutagens such as ethyl methanesulfonate (EMS) were more popular as they are less destructive, easier available, and have a higher efficiency than physical mutagens [9]. Theoretically, we can find a EMS mutation in any given gene by screening no more than 5000 plants from the mutagenized M1 generation for the model plant Arabidopsis [3,8]. Conventional mutagenesis has been widely used in forward genetic strategies that start with a phenotype of interest and address identification of genes affecting this phenotype [9][10][11].As an alternative forward-genetic tool, insertional mutagenesis, including T-DNA (Transferred DNA) and transposon tagging, facilitate the identification of genes disrupted by these elements [5]. Currently, T-DNA-tagged lines have been generated in large numbers, becoming a popular resource for plant gene function [12,13].Superior to T-DNA, mobilizable transponsons can provide a verification about mutational effects of insertions when they are remobilized from the insertion site to recover a potential phenotype [14]. Insertional mutagenesis is less practicable for species, for which a systematic transformation platform has not been established [15].As the number of characterized plant genes increases, reverse-genetic methodologies play an increasingly important role in gene function validation [16]. Targeting Induced Local Lesions in Genomes (TILLING) is the first reverse-genetic tool, in which chemical or physical mutagenesis is followed by a high-throughput screening for point mutations [3,4,16]. TILLING is practicable for plant species with large-sized genomes and without transformation system because it is not different from traditional chemical or physical mutagenesis in creating mutations [12].Changes of gene expression levels may result in modified phenotypes, which can be another powerful approach for elucidating gene function [5...

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Highly Efficient Doubled-Haploid Production in Wheat ( L.) via Induced Microspore Embryogenesis

Cited by 58 publications

References 0 publications

Effects of exogenous application of polyamines on wheat anther cultures

Effects of exogenous application of polyamines on wheat anther cultures

Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Haploid Strategies for Functional Validation of Plant Genes

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