Highly efficient approach for characterizing nanometer-sized gold particles by capillary electrophoresis

Liu, Fu‐Ken; Lin, Yuan-Yao; Wu, Chueh‐Yu

doi:10.1016/j.aca.2004.08.052

Cited by 87 publications

(57 citation statements)

References 20 publications

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“…Addition of surfactant to the separation buffer results in an inversion of the migration order, due to an increase in ka. These results were confirmed in a later study by Liu et al [38]. For SDS-stabilized gold nanoparticles, it has been also shown that those on-line enrichment principles (taking large-volume stacking as an example) applicable to molecular analytes can also be applied to charged nanoparticles [39,40].…”

Section: Coagulationmentioning

confidence: 62%

Characterization of nanoparticles by capillary electromigration separation techniques

Pyell¹

2010

Electrophoresis

133

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Considering the large number of papers presented in the last years in the field of CE and plate gel electrophoresis (PGE) of nanoparticles and nanoparticle-biomolecule conjugates, this review article gives an overview on the application of capillary electromigration separation techniques for the separation of nanoparticles and nanoparticle-biomolecule conjugates according to differences in size and/or zeta potential and for the determination of several physicochemical parameters characterizing a population of nanoparticles. A brief introduction is given into the theoretical background of the size-selective electrophoretic separation of nanospheres in free-solution and sieving media including involvement of interparticle interactions. Some aspects of colloidal system stability are discussed. Applications of capillary electromigration techniques in the field of size-selective and/or surface charge density-selective separation of nanoparticles and nanoparticle-biomolecule conjugates are described, including free-solution CE, CGE, IEF, and ITP, complemented by an overview on the application of (semipreparative) PGE in the field of nanotechnology and nanobiotechnology. The review also includes actual developments regarding the coupling of capillary electromigration techniques to (wavelength-resolved) LIF and other selective detection methods and studies concerning individual-particle CE.

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Section: Coagulationmentioning

confidence: 62%

Characterization of nanoparticles by capillary electromigration separation techniques

Pyell¹

2010

Electrophoresis

133

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“…18 Therefore, once again I chose SDS and CAPS mixtures to be my MEKC running electrolytes in this study.…”

Section: Resultsmentioning

confidence: 99%

“…[16][17][18][19] In previous papers, we reported that electrophoresis systems can be used to separate Au NPs based on their sizes. 18 Thus, a single sample can be rapidly analyzed using a volume as low as several nanoliters, with simultaneous characterization of the size-dependent properties of its NPs; 19 this approach is more efficient than the use of either microscopy or DLS for the size characterization of Au NPs.…”

Section: Introductionmentioning

confidence: 99%

Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

Liu

2010

ANAL. SCI.

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“…Finally, they are individually collected from two or more outlets at the fractionation segment. A hydrodynamic focusing technique such as sheath flow has been often used to focus the particles, and migration techniques of the particles, such as electrophoresis, 11,12 dielectrophoresis (DEP), 9,13-15 magnetophoresis, [16][17][18] acoustophoresis, [19][20][21] dean flow, 22 pinched flow fractionation (PFF), 10,23 and split-flow thin (SPLITT) fractionation, [24][25][26] have been utilized to separate them based on the difference of their migration velocities in the flow. Among them, the method in which the particles are separated in the orthogonal direction to the flow and fractioned at each outlet is called a free-flow technique.…”

Section: Introductionmentioning

confidence: 99%

Staggered-electromagnetophoresis with a Split-flow System for the Separation of Microparticles by a Hollow Fiber-embedded PDMS Microchip

et al. 2016

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Recently, we have developed a new microchip separation method for microparticles utilizing electromagnetophoresis (EMP) under the concept of the free-flow technique. 27 EMP, which was the migration phenomenon based on the Lorentz force proposed for the first time by Kolin in 1953, 28 is a force induced on a particle placed in a magnetic and electric field 2016 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. A novel microchip separation system for microparticles based on electromagnetophoresis (EMP) was developed. In this system, focusing and separation of flowing microparticles in a microchannel could be performed by staggered-EMP by controlling the electric current applied to the channel locally combined with the split-flow system for fractionation of eluates. To apply the electric current through the flushing medium in the microchannel, a hollow fiber-embedded microchip with multiple electrodes was fabricated. The hollow fiber was made by a semi-permeable membrane and could separate small molecules. This microchip allowed us to apply the electric current to a part of the microchannel without any pressure control device because a main channel contacted with the subchannels that had electrodes through the semi-permeable membrane. Moreover, the separation using this microchip was combined with the split-flow system at two outlets to improve separation efficiency. Using this system, with the split-flow ratio of 10:1, 87% of 3 μm polystyrene (PS) latex particles were isolated from a mixture of 3 and 10 μm particles. Even the separation of 6 and 10 μm PS particles was achieved with about 77% recovery and 100% purity. In addition, by controlling the applied current, size fractionation of polypropylene (PP) particles was demonstrated. Moreover, biological particles such as pollens could be separated with high separation efficiency by this technique.

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Highly efficient approach for characterizing nanometer-sized gold particles by capillary electrophoresis

Cited by 87 publications

References 20 publications

Characterization of nanoparticles by capillary electromigration separation techniques

Characterization of nanoparticles by capillary electromigration separation techniques

Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

Staggered-electromagnetophoresis with a Split-flow System for the Separation of Microparticles by a Hollow Fiber-embedded PDMS Microchip

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