Highly biocompatible and recyclable biomimetic nanoparticles for antibiotic-resistant bacteria infection

Chen, Bei; Li, Fangfang; Zhu, Xin Kai; Hu, Xue; Zan, Ming Hui; Li, XueKe; Li, Qian-Ying; Guo, Shishang; Zhao, Xingzhong; Jiang, Yingan; Cao, Zhijian; Liu, Wei

doi:10.1039/d0bm01397h

Cited by 32 publications

(23 citation statements)

References 41 publications

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“…The RBCM provided the preparation with toxins clearance and immune escaping capability [35]. For MRSA infected wound, a RBCM-coated Fe 3 O 4 nanoparticles (RBC@Fe 3 O 4 ) had superior treatment efficacy than the Fe 3 O 4 itself [42]. The RBC@Fe 3 O 4 acted as nano-sponges to trap bacterial toxins and then kill them all with a photothermal effect of Fe 3 O 4 .…”

Section: Discussionmentioning

confidence: 99%

Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy

Raza

et al. 2021

Pharmaceutics

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Multiple drug resistance (MDR) in bacterial infections is developed with the abuse of antibiotics, posing a severe threat to global health. Tedizolid phosphate (TR-701) is an efficient prodrug of tedizolid (TR-700) against gram-positive bacteria, including methicillin-sensitive staphylococcus aureus (MSSA) and methicillin-resistant staphylococcus aureus (MRSA). Herein, a novel drug delivery system: Red blood cell membrane (RBCM) coated TR-701-loaded polylactic acid-glycolic acid copolymer (PLGA) nanoparticles (RBCM-PLGA-TR-701NPs, RPTR-701Ns) was proposed. The RPTR-701Ns possessed a double-layer core-shell structure with 192.50 ± 5.85 nm in size, an average encapsulation efficiency of 36.63% and a 48 h-sustained release in vitro. Superior bio-compatibility was confirmed with red blood cells (RBCs) and HEK 293 cells. Due to the RBCM coating, RPTR-701Ns on one hand significantly reduced phagocytosis by RAW 264.7 cells as compared to PTR-701Ns, showing an immune escape effect. On the other hand, RPTR-701Ns had an advanced exotoxins neutralization ability, which helped reduce the damage of MRSA exotoxins to RBCs by 17.13%. Furthermore, excellent in vivo bacteria elimination and promoted wound healing were observed of RPTR-701Ns with a MRSA-infected mice model without causing toxicity. In summary, the novel delivery system provides a synergistic antibacterial treatment of both sustained release and bacterial toxins absorption, facilitating the incorporation of TR-701 into modern nanotechnology.

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Section: Discussionmentioning

confidence: 99%

Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy

Raza

et al. 2021

Pharmaceutics

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“…[ 43 ] As an anti‐virulence platform, RBC‐NS were also applied to neutralize whole secreted proteins (wSP) of bacteria. [ 44,45 ] In this study, RBC‐NS inhibited wSP‐induced cytotoxicity on human umbilical vein endothelial cells and hemolysis in vitro ( Figure ). [ 44 ] When the mice were challenged with a lethal dose of wSP, the RBC‐NS increased the mouse survival rate.…”

Section: Neutralizing Bacterial Toxinsmentioning

confidence: 69%

“…In a subcutaneous MRSA infection mouse model, the skin lesion development of mice treated with NS‐gel was slower than those treated with hydrogel without embedded nanosponges. Meanwhile, RBC membrane was coated onto other nanoparticle cores, such as hydrogel nanoparticles, [ 47 ] iron oxide, [ 45 ] and anisotropic polymeric nanoparticles, [ 48 ] for toxin neutralization. In the above examples, PLGA cores stand out for their high encapsulation efficiency of hydrophobic payloads and controlled‐release properties, and hydrogel cores are especially suited for encapsulating hydrophilic drugs and delivering them in response to environmental cues.…”

Section: Neutralizing Bacterial Toxinsmentioning

confidence: 99%

Cellular Nanosponges for Biological Neutralization

Wang

Duan

et al. 2022

Advanced Materials

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In viral infections, viral particles attach themselves to the host cell membrane for invasion, hijacking host cell machinery to replicate. [9,10] In order to counteract these threats, biological neutralization represents a general strategy that deploys therapeutic agents to bind with the harmful molecules or pathogens, block their bioactivity, and thus prevent them from causing the diseases. [11][12][13][14] Therapeutic platforms, such as antisera, monoclonal antibodies, and small-molecule inhibitors, have been widely used for neutralization. [15][16][17][18][19] Despite their pivotal roles in treating numerous diseases, these platforms sometimes show inadequate efficacy. The reason is attributable, in part, to their design of focusing on the causative molecules or pathogens, which leads to narrow-spectrum neutralization solutions. For example, some antidotes are highly effective in treating poisoning, but most deadly toxicants do not have specific pharmacological antidotes. [12] In inflammatory disorders, existing agents neutralizing one or a few cytokines are insufficient to halt or reverse disease progression due to the multiplicity of cytokine targets and signaling network redundancy. [20] In bacterial infections, bacterial toxins display enormous diversity of molecular structures and epitopic targets. [21,22] In contrast, current neutralizing agents target specific toxin structures and require customized design for different toxins, making their wide applications impractical. Similarly, viral neutralization focuses heavily on specific viral species and therefore cannot be deployed across different species or families of viruses or may be rendered ineffective as the virus accumulates mutations and escapes treatments. [23] Overall, these challenges underscore the need for innovative biological neutralization approaches.Recently, the emergency of cell-membrane-coated nanoparticles offers a unique solution to address the challenges facing current biological neutralization technologies. [24] These nanoparticles are made by first deriving natural cell membranes and then coating them onto synthetic cores. Cell-membranecoated nanoparticles were first developed by coating red blood cell (RBC) membranes onto polymeric cores. [25] The goal was to replicate the long-circulation feature of natural RBCs desirable for drug delivery. However, the success of these biomimetic nanoparticles soon sparked the idea of using them as decoys of susceptible RBCs for biological neutralization. [26] All pathological agents must interact with host cells for their bioactivity.Biological neutralization represents a general strategy that deploys therapeutic agents to bind with harmful molecules or infectious pathogens, block their bioactivity, and thus prevent them from causing the diseases. Here, a comprehensive review of using cell-membrane-coated nanoparticles, namely "cellular nanosponges," as host decoys for a wide range of biological neutralization applications is provided. Compared to traditional neutralization strategies, the cellu...

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“…At concentrations of 20, 80, 320, and > 320 μg/mL, selenium nanostructures effectively demonstrated cytotoxicity against MDR bacteria and they also showed synergistic impact in combination therapy with linezolid as a conventional antibiotic 50 . Therefore, nanostructures can be employed for elimination, inactivation and sterilization purposes and bacteria cannot develop resistance to these cytotoxic agents 51 , 52 .…”

Section: Resultsmentioning

confidence: 99%

Multifunctional green synthesized Cu–Al layered double hydroxide (LDH) nanoparticles: anti-cancer and antibacterial activities

Kiani

Bagherzadeh

Ghadiri

et al. 2022

Sci Rep

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Doxorubicin (DOX) is a potent anti-cancer agent and there have been attempts in developing nanostructures for its delivery to tumor cells. The nanoparticles promote cytotoxicity of DOX against tumor cells and in turn, they reduce adverse impacts on normal cells. The safety profile of nanostructures is an important topic and recently, the green synthesis of nanoparticles has obtained much attention for the preparation of biocompatible carriers. In the present study, we prepared layered double hydroxide (LDH) nanostructures for doxorubicin (DOX) delivery. The Cu–Al LDH nanoparticles were synthesized by combining Cu(NO3)2·3H2O and Al(NO3)3·9H2O, and then, autoclave at 110. The green modification of LDH nanoparticles with Plantago ovata (PO) was performed and finally, DOX was loaded onto nanostructures. The FTIR, XRD, and FESEM were employed for the characterization of LDH nanoparticles, confirming their proper synthesis. The drug release study revealed the pH-sensitive release of DOX (highest release at pH 5.5) and prolonged DOX release due to PO modification. Furthermore, MTT assay revealed improved biocompatibility of Cu–Al LDH nanostructures upon PO modification and showed controlled and low cytotoxicity towards a wide range of cell lines. The CLSM demonstrated cellular uptake of nanoparticles, both in the HEK-293 and MCF-7 cell lines; however, the results were showed promising cellular internalizations to the HEK-293 rather than MCF-7 cells. The in vivo experiment highlighted the normal histopathological structure of kidneys and no side effects of nanoparticles, further confirming their safety profile and potential as promising nano-scale delivery systems. Finally, antibacterial test revealed toxicity of PO-modified Cu–Al LDH nanoparticles against Gram-positive and -negative bacteria.

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Highly biocompatible and recyclable biomimetic nanoparticles for antibiotic-resistant bacteria infection

Abstract: Highly biocompatible biomimetic nanoparticles for antibiotic-resistant bacteria infection by photothermal therapy.

Cited by 32 publications

References 41 publications

Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy

Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy

Cellular Nanosponges for Biological Neutralization

Multifunctional green synthesized Cu–Al layered double hydroxide (LDH) nanoparticles: anti-cancer and antibacterial activities

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