Highly active Na+, K+-ATPase in rat submaxillary gland bearing on salivary secretion

Schwartz, A; Moore, Charles A.

doi:10.1152/ajplegacy.1968.214.5.1163

Cited by 35 publications

(7 citation statements)

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“…As pointed out by Stirling (1972) the ratio between specific and non-specific binding of ouabain is likely to be very high in tissues with a high content of (Na++K+)activated ATPase. Furthermore, it has been shown that non-specific binding of ouabain is relatively low at the concentration used in the present experiments, 10-M, even in the case of HeLa cells, which contain less (Na+ + K+)-activated ATPase than salivary gland cells (Baker & Willis, 1969Schwartz, Laseter & Kraintz, 1963;Schwartz & Moore, 1968).…”

Section: Discussionmentioning

confidence: 60%

“…This view is supported by the absence of such ducts in glands secreting isotonic saliva (Thaysen, 1955;Lundberg, 1957). Schwartz & Moore (1968) have demonstrated the existence of a highly active (Na+ + K+)-activated ATPase in the rat submandibular gland. This finding is important, since it has been shown that salivary secretion, reabsorption of sodium, and active post-stimulatory re-uptake of potassium and extrusion of sodium can all be inhibited by ouabain (Mangos & Braun, 1966;Petersen & Poulsen, 1967;Petersen, 1971;Poulsen, 1974a, b).…”

Section: Introductionmentioning

confidence: 95%

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Localization of sodium pump sites in cat salivary glands.

Bundgaard¹,

Møller²,

Poulsen³

1977

The Journal of Physiology

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5. Electron microscopy revealed that the demilunar cells possessed long, tortuous intercellular spaces separated from the secretary canaliculi by junctional complexes. In contrast, the membranes between adjacent central acinar cells were fairly straight.6. It is concluded that the striated ducts play a dominant role in the ductal reabsorption of sodium, and that the transport is probably mediated by a (Na++K+)-activated ATPase. The findings on the acinar level are in agreement with the view that the primary saliva is formed predominantly by the demilunar cells. The role of a (Na+ + K+)-activated ATPase in this process is unclear.

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Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 95%

Localization of sodium pump sites in cat salivary glands.

Bundgaard¹,

Møller²,

Poulsen³

1977

The Journal of Physiology

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“…3), seems to indicate that this K transport is mediated by a Na-K-activated ATP-ase (Skou, 1965). The existence of Na-Kactivated ATP-ase in the dog parotid and rat submandibular gland has been demonstrated by Schwartz, Laseter & Kraintz (1963) and Schwartz & Moore (1968). The stimulus for the K pump would seem to be an increased intracellular Na concentration.…”

Section: H Petersenmentioning

confidence: 84%

“…The stimulus for the K pump would seem to be an increased intracellular Na concentration. During perfusion with Li Locke solution, however, no Na can enter the gland cells during ACh stimulation and as Li exerts no stimulatory effect at the Na site of the ATP-ase (Schwartz & Moore, 1968) no K uptake can occur. If this idea were correct one would expect Li to accumulate in the gland cells in the same manner as in the frog skin (Zerahn, 1955).…”

Section: H Petersenmentioning

confidence: 99%

Some factors influencing stimulation‐induced release of potassium from the cat submandibular gland to fluid perfused through the gland

Petersen¹

1970

The Journal of Physiology

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SUMMARY1. The release of K from the cat submandibular gland to the extracellular fluid (ECF) after stimulation with acetylcholine (ACh) and the subsequent uptake of K from the ECF was studied in glands perfused artificially with Locke solutions.2. The first injection of ACh after shift of the perfusion fluid from control to K-free Locke solution evoked a normal loss of K and a normal secretion of saliva. The second injection only evoked a small release of K and a reduced secretion.3. Perfusion with dinitrophenol (DNP) (104 M) containing solutions, Nafree Li Locke solutions and chloride-free nitrate Locke solutions inhibited salivary secretion and the uptake of K.

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“…The existence of Na +/K + ATPase was reported in the rat mandibular gland (SCHWARTZ and MOORE, 1968;BOGART, 1975), and there are many reports on the inhibitory effects by ouabain on salivary secretion (PETERSEN and PoULSEN, 1967;MARTINEZ, 1971;MARTINEZ and CASSITY, 1983) and on Na+ dependency of salivary secretion (PETERSEN, 1970;CASE and HUNTER, 1982). BURGEN (1956) andIMAI (1965) reported that ACh increased the Na + entry in the salivary gland.…”

Section: Discussionmentioning

confidence: 99%

31P NMR studies on the isolated perfused mandibular gland of the rat.

et al. 1987

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Phosphorus nuclear magnetic resonance (31P NMR) was used to study energy metabolism in the rat mandibular gland. The gland was isolated, perfused arterially and set in the NMR tube. At rest, 7 resonance peaks were observed and 6 peaks identified from low field as: 1) sugar phosphates (SP) and nucleotide monophosphate (NMP), 2) inorganic phosphate (Pi), 3) creatine phosphate (PCr), 4) y-nucleotide triphosphate (NTP) and f3-nucleotide diphosphate (NDP), 5) a-NTP, a-NDP, NAD+, and NADH, 6) an unknown peak, and 7) 13-NTP. From the results of high performance liquid chromatography (HPLC), NTP consisted mainly of ATP and GTP, and UTP was not detected. The tissue contents of ATP and GTP in the perfused gland were determined by HPLC as 1.86 + 0.03 and 0.37 + 0.01 mmol/kg wet tissue (S.E., n = 5). From 31P NMR and HPLC data, the tissue levels of creatine phosphate, ADP, and sugar phosphates were estimated as 3.3, 0.4, and 4.2 mmol/kg wet tissue, respectively. The cessation of perfusion decreased the tissue levels of PCr and ATP and increased those of Pi and SP. On the other hand, administration of acetylcholine (1 ELM), which is an optimal dose for secretion, decreased PCr and increased Pi but did not change SP. The ATP was unchanged initially and slowly decreased to the lower level during sustained secretion. These findings suggest that a sustained secretion requires more energy from ATP hydrolysis rather than initial secretion.

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Highly active Na+, K+-ATPase in rat submaxillary gland bearing on salivary secretion

Cited by 35 publications

References 0 publications

Localization of sodium pump sites in cat salivary glands.

Localization of sodium pump sites in cat salivary glands.

Some factors influencing stimulation‐induced release of potassium from the cat submandibular gland to fluid perfused through the gland

31P NMR studies on the isolated perfused mandibular gland of the rat.

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