Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation

Lamme, Evert N.; Leeuwen, R.T.J. van; Brandsma, Kor; Marle, J. van; Middelkoop, Esther

doi:10.1002/(sici)1096-9896(200004)190:5<595::aid-path572>3.0.co;2-v

Cited by 158 publications

(118 citation statements)

References 30 publications

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“…It has been reported that fibroblasts seeded in collagen sponge inhibit host fibroblast infiltration [54,55] and secrete basic fibroblast growth factor that induces the apoptosis of myofibroblasts [56]. Both phenomena might contribute to the reduction of skin wound contraction.…”

Section: Discussionmentioning

confidence: 99%

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Leirós

Kusinsky

Drago³

et al. 2014

Stem Cells Translational Medicine

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Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and might contribute to the understanding of skin regeneration. The use of dermal papilla cells (DPCs) as a dermal component in a permanent composite skin with human hair follicle stem cells (HFSCs) was evaluated by studying the tissue-engineered skin architecture, stem cell persistence, hair regeneration, and graft-take in nude mice. A porcine acellular dermal matrix was seeded with HFSCs alone and with HFSCs plus human DPCs or dermal fibroblasts (DFs). In vitro, the presence of DPCs induced a more regular and multilayered stratified epidermis with more basal p63-positive cells and invaginations. The DPC-containing constructs more accurately mimicked the skin architecture by properly stratifying the differentiating HFSCs and developing a well-ordered epithelia that contributed to more closely recapitulate an artificial human skin. This acellular dermal matrix previously repopulated in vitro with HFSCs and DFs or DPCs as the dermal component was grafted in nude mice. The presence of DPCs in the composite substitute not only favored early neovascularization, good assimilation and remodeling after grafting but also contributed to the neovascular network maturation, which might reduce the inflammation process, resulting in a better healing process, with less scarring and wound contraction. Interestingly, only DPC-containing constructs showed embryonic hair bud-like structures with cells of human origin, presence of precursor epithelial cells, and expression of a hair differentiation marker. Although preliminary, these findings have demonstrated the importance of the presence of DPCs for proper skin repair.

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Section: Discussionmentioning

confidence: 99%

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Leirós

Kusinsky

Drago³

et al. 2014

Stem Cells Translational Medicine

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“…It is stated that CAPE enhances fibroblast activity; in this way increases the wound healing process. 31 Song et al reported that CAPE increase collagen-like polymer levels secreted by fibroblasts. 32 Tanimoto et al (2012) and Bushra Habeeb Al-Molla (2014) found that propolis enhanced the bone formation in one week and stated that amelogenins increases the mineralisation accompanied by the up-regulation of bone markers in human bone marrow MSCs during osteogenic differentiation.…”

Section: Summary Of the Studies Includedmentioning

confidence: 85%

Effect of Propolis on Bone Loss Prevention- A Review

Sowmya¹,

Gujjari²,

Mruthunjaya³

et al. 2017

jemds

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BACKGROUND Statement of problem-Bone loss caused by excessive osteoclast formation is the major mechanism underlying many bone diseases leading to problems in dentistry, affecting the treatment plan and patient's quality of life. Despite many treatment options, a development of novel therapies that are natural, safe, effective and economical is vital for improved strategies against bone loss. Propolis, a natural material that honeybees collect from living plants has been reported to have multiple biological effects including bone loss prevention activities. But there is no systematic review available that has evaluated the effect of propolis on bone loss prevention. MATERIALS AND METHODSThe articles with the clinical and in vitro investigations that assessed the prevention and treatment of bone loss using propolis were selected. Electronic database was searched from 1978 to 2016 using the keywords "Propolis" or "propolis" and "bone loss" or "propolis" and "prevention of bone loss." RESULTS Propolis, which contains a component called Caffeic Acid Phenethyl Ester (CAPE)has an effect on prevention of bone loss. The mechanism of action of CAPE is by inhibiting NF-kappa B and NFAT activation. This in turn results in attenuation of osteoclastogenesis and bone resorption, implying that CAPE can be potential treatment for bone loss prevention. CONCLUSIONThe literature review suggests that the use of propolis can prevent bone loss. However, as most of the studies were in vitro, there is insufficient reliable evidence to truly provide a recommendation regarding this natural compound. A well-designed, randomised controlled trials are needed to provide answers to these questions.

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“…In an in vivo porcine model of cutaneous wound repair, we have recently successfully shown that contraction of wounds is significantly reduced when full thickness wounds are treated with a combination of autologous keratinocytes and fibroblasts delivered to the wound bed on microcarriers, compared with wounds that were treated either with keratinocytes alone or with an ultra-thin skin graft (Martin et al 2011). Other groups have previously reported that full thickness wounds treated with dermal substitutes seeded with autologous fibroblasts contracted less than those treated with dermal substitutes alone (Lamme et al 2000). We, and others, have speculated that the presence of additional cells delivered to the wound bed may reduce the need for host fibroblasts of the contractile myofibroblast phenotype to migrate into the remodelling dermis, thus reducing the contractile tension on the healing wound (Lamme et al 2000;Martin et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Other groups have previously reported that full thickness wounds treated with dermal substitutes seeded with autologous fibroblasts contracted less than those treated with dermal substitutes alone (Lamme et al 2000). We, and others, have speculated that the presence of additional cells delivered to the wound bed may reduce the need for host fibroblasts of the contractile myofibroblast phenotype to migrate into the remodelling dermis, thus reducing the contractile tension on the healing wound (Lamme et al 2000;Martin et al 2011). …”

Section: Discussionmentioning

confidence: 99%

A fully autologous co-culture system utilising non-irradiated autologous fibroblasts to support the expansion of human keratinocytes for clinical use

et al. 2011

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Autologous keratinocytes can be used to augment cutaneous repair, such as in the treatment of severe burns and recalcitrant ulcers. Such cells can be delivered to the wound bed either as a confluent sheet of cells or in single-cell suspension. The standard method for expanding primary human keratinocytes in culture uses lethally irradiated mouse 3T3 fibroblasts as feeder cells to support keratinocyte attachment and growth. In an effort to eliminate xenobiotic cells from clinical culture protocols where keratinocytes are applied to patients, we investigated whether human autologous primary fibroblasts could be used to expand keratinocytes in culture. At a defined ratio of a 6:1 excess of keratinocytes to fibroblasts, this co-culture method displayed a population doubling rate comparable to culture with lethally irradiated 3T3 cells. Furthermore, morphological and molecular analysis showed that human keratinocytes expanded in co-culture with autologous human fibroblasts were positive for proliferation markers and negative for differentiation markers. Keratinocytes expanded by this method thus retain their proliferative phenotype, an important feature in enhancing rapid wound closure. We suggest that this novel co-culture method is therefore suitable for clinical use as it dispenses with the need for lethally irradiated 3T3 cells in the rapid expansion of autologous human keratinocytes.

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Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation

Cited by 158 publications

References 30 publications

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Effect of Propolis on Bone Loss Prevention- A Review

A fully autologous co-culture system utilising non-irradiated autologous fibroblasts to support the expansion of human keratinocytes for clinical use

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