Higher frequency of vertebrate‐infecting viruses in the gut of infants born to mothers with type 1 diabetes

Kim, Ki Wook; Allen, Digby W.; Briese, Thomas; Couper, Jennifer; Barry, Simon C.; Colman, Peter; Cotterill, Andrew; Davis, Elizabeth A.; Giles, Lynne; Harrison, Leonard C.; Harris, Mark; Haynes, Aveni; Horton, Jessica L.; Isaacs, Sonia R; Jain, Komal; Lipkin, W. Ian; McGorm, Kelly; Morahan, Grant; Morbey, Claire; Pang, Ignatius C. N.; Papenfuss, Anthony T.; Penno, Megan A. S.; Sinnott, Richard; Soldatos, Georgia; Thomson, Rebecca L.; Vuillermin, Peter; Wentworth, John M.; Wilkins, Marc R.; Rawlinson, William D.; Craig, Maria E.

doi:10.1111/pedi.12952

Cited by 13 publications

(13 citation statements)

References 52 publications

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“…Among other respiratory viruses, picornaviruses (all of which were rhinoviruses) were detected in 5% (5/92) of cases and in nCoV_neg_1, and influenzavirus A in 2% (2/92) of cases. Consistent with our published data in other cohorts 9,10,11 , the non-respiratory viruses detected included mammarenaviruses (41%), roseoloviruses (36%), alphapolyomaviruses (35%), papillomaviruses (20%), lymphocryptoviruses (12%), lentivruses (9%), anelloviruses (3%), simplexviruses (3%), pestiviruses (3%), mastadenovirus (1%) and norovirus (1%). Overall, sequences of viruses other than SARS-CoV-2 were detected in 74% (68/92) of cases, and the rate of co-infection between SARS-CoV-2 and other respiratory viruses was 8% (7/92).…”

Section: Rate Of Viral Co-infection In Australian Casessupporting

confidence: 89%

“…For VirCapSeq hybrid-capture, individual libraries (92 cases, 2 coronavirus-negative controls and 2 salmon sperm DNA controls) were combined by equal mass into two capture pools (48-plex) and hybridized to probes (VirCapSeq-VERT design; Roche) for 16 hours as previously described 11 , following the SeqCap EZ HyperCap Worklfow v2.3 (Roche). For hybrid-capture using the Twist Respiratory Virus Research Panel (103067; Twist Biosciences, San Francisco, CA), 85 libraries (83 cases and 2 coronavirus-negative controls) were combined by equal mass into five capture pools (16-to 20-plex; average 17-plex).…”

Section: Target Enrichment Sequencingmentioning

confidence: 99%

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Respiratory viral co-infections among SARS-CoV-2 cases confirmed by virome capture sequencing

Kim

Pang

Yeang

et al. 2020

Preprint

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Accumulating evidence supports the high prevalence of co-infections among Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) patients, and their potential to worsen the clinical outcome of COVID-19. However, there are few data on Southern Hemisphere populations, and most studies to date have investigated a narrow spectrum of viruses using targeted qRT-PCR. Here we assessed respiratory viral co-infections among SARS-CoV-2 patients in Australia, through respiratory virome characterization. Nasopharyngeal swabs of 92 SARS-CoV-2-positive cases were sequenced using pan-viral hybrid-capture and the Twist Respiratory Virus Panel. In total, 8% of cases were co-infected, with rhinovirus (6%) or influenzavirus (2%). Twist capture also achieved near-complete sequencing (>90% coverage, >10-fold depth) of the SARS-CoV-2 genome in 95% of specimens with Ct<30. Our results highlight the importance of assessing all pathogens in symptomatic patients, and the dual-functionality of Twist hybrid-capture, for SARS-CoV-2 whole-genome sequencing without amplicon generation and the simultaneous identification of viral co-infections with ease.

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Section: Rate Of Viral Co-infection In Australian Casessupporting

confidence: 89%

Section: Target Enrichment Sequencingmentioning

confidence: 99%

Respiratory viral co-infections among SARS-CoV-2 cases confirmed by virome capture sequencing

Kim

Pang

Yeang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…This indicated a previously unrecognised association of IA with higher EV-A abundance in the gut of children with a first-degree relative with T1D [ 205 ]. VirCapSeq-VERT has since been successfully used to elucidate distinct pregnancy gut virome profiles in women with T1D versus without [ 241 ] and between infants born from mothers with T1D versus without in the ENDIA study in the first year after birth [ 242 ].…”

Section: Virome and T1dmentioning

confidence: 99%

Viruses and Type 1 Diabetes: From Enteroviruses to the Virome

et al. 2021

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For over a century, viruses have left a long trail of evidence implicating them as frequent suspects in the development of type 1 diabetes. Through vigorous interrogation of viral infections in individuals with islet autoimmunity and type 1 diabetes using serological and molecular virus detection methods, as well as mechanistic studies of virus-infected human pancreatic β-cells, the prime suspects have been narrowed down to predominantly human enteroviruses. Here, we provide a comprehensive overview of evidence supporting the hypothesised role of enteroviruses in the development of islet autoimmunity and type 1 diabetes. We also discuss concerns over the historical focus and investigation bias toward enteroviruses and summarise current unbiased efforts aimed at characterising the complete population of viruses (the “virome”) contributing early in life to the development of islet autoimmunity and type 1 diabetes. Finally, we review the range of vaccine and antiviral drug candidates currently being evaluated in clinical trials for the prevention and potential treatment of type 1 diabetes.

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“…This indicated a previously unrecognised association of IA with higher EV-A abundance in the gut of children with a first degree relative with T1D [201]. VirCapSeq-VERT has since been successfully used to elucidate distinct pregnancy gut virome profile in women with T1D versus without [237], and between infants born from mothers with T1D versus without in the ENDIA study in the first year after birth [238].…”

Section: Virome and T1dmentioning

confidence: 96%

Viruses and Type 1 Diabetes: From Enteroviruses to the Virome

Isaacs¹,

Foskett²,

Maxwell³

et al. 2021

Preprint

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For over a century, viruses have left a long trail of evidence implicating them as frequent suspects in the development of type 1 diabetes. Through vigorous interrogation of viral infections in individuals with islet autoimmunity and type 1 diabetes using serological and molecular virus detection methods, and mechanistic studies of virus infected human pancreatic β-cells, the prime suspects have been narrowed down to predominantly human enteroviruses. Here we provide a comprehensive overview of evidence supporting the hypothesised role of enteroviruses in the development of islet autoimmunity and type 1 diabetes. We also discuss concerns over the historical focus and investigation bias toward enteroviruses, and summarise current unbiased efforts aimed at characterising the complete population of viruses (the “virome”) contributing early in life to the development of islet autoimmunity and type 1 diabetes. Finally, we review the range of vaccine and antiviral drug candidates currently being evaluated in clinical trials for the prevention and potential treatment of type 1 diabetes.

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Higher frequency of vertebrate‐infecting viruses in the gut of infants born to mothers with type 1 diabetes

Cited by 13 publications

References 52 publications

Respiratory viral co-infections among SARS-CoV-2 cases confirmed by virome capture sequencing

Respiratory viral co-infections among SARS-CoV-2 cases confirmed by virome capture sequencing

Viruses and Type 1 Diabetes: From Enteroviruses to the Virome

Viruses and Type 1 Diabetes: From Enteroviruses to the Virome

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