High Zn and Cd accumulation in the oyster Crassostrea rhizophorae, and its relevance as a sentinel species

Rebelo, Mauro de Freitas; Amaral, Maria Clara Rebouças do; Pfeiffer, Wolfgang C.

doi:10.1016/s0025-326x(03)00244-3

Cited by 29 publications

(14 citation statements)

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“…MTs play roles in the metabolism of non-toxic essential metals (zinc and copper), as well as toxic heavy metals (cadmium, mercury, etc) [ 50 ]. Species of the genus Crassostrea have been used in ecotoxicological bioassays to assess the bioaccumulation of Cd and Zn in field conditions [ 51 ] and in transplant assays [ 52 , 53 ]. When comparing the genome of C. angulata with that of C. gigas , the GO terms (which describe molecular function) associated with binding to ions of zinc and other metals present in C. angulata account for the largest number of polymorphic genes (Single nucleotide polymorphisms: SNP) observed [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

A preliminary integrated genetic map distinguishes every chromosome pair and locates essential genes related to abiotic adaptation of Crassostrea angulata/gigas

et al. 2018

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BackgroundThe re-sequencing of C. angulata has revealed many polymorphisms in candidate genes related to adaptation to abiotic stress that are not present in C. gigas; these genes, therefore, are probably related to the ability of this oyster to retain high concentrations of toxic heavy metals. There is, in addition, an unresolved controversy as to whether or not C. angulata and C. gigas are the same species or subspecies. Both oysters have 20 metacentric chromosomes of similar size that are morphologically indistinguishable. From a genomic perspective, as a result of the great variation and selection for heterozygotes in C. gigas, the assembly of its draft genome was difficult: it is fragmented in more than seven thousand scaffolds.ResultsIn this work sixty BAC sequences of C. gigas downloaded from NCBI were assembled in BAC-contigs and assigned to BACs that were used as probes for mFISH in C. angulata and C. gigas. In addition, probes of H3, H4 histone, 18S and 5S rDNA genes were also used. Hence we obtained markers identifying 8 out the 10 chromosomes constituting the karyotype. Chromosomes 1 and 9 can be distinguished morphologically. The bioinformatic analysis carried out with the BAC-contigs annotated 88 genes. As a result, genes associated with abiotic adaptation, such as metallothioneins, have been positioned in the genome. The gene ontology analysis has also shown many molecular functions related to metal ion binding, a phenomenon associated with detoxification processes that are characteristic in oysters. Hence the provisional integrated map obtained in this study is a useful complementary tool for the study of oyster genomes.ConclusionsIn this study 8 out of 10 chromosome pairs of Crassostrea angulata/gigas were identified using BAC clones as probes. As a result all chromosomes can now be distinguished. Moreover, FISH showed that H3 and H4 co-localized in two pairs of chromosomes different that those previously escribed. 88 genes were annotated in the BAC-contigs most of them related with Molecular Functions of protein binding, related to the resistance of the species to abiotic stress. An integrated genetic map anchored to the genome has been obtained in which the BAC-contigs structure were not concordant with the gene structure of the C. gigas scaffolds displayed in the Genomicus database.Electronic supplementary materialThe online version of this article (10.1186/s12863-018-0689-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

A preliminary integrated genetic map distinguishes every chromosome pair and locates essential genes related to abiotic adaptation of Crassostrea angulata/gigas

et al. 2018

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“…Hydrogen-ion concentration [pH]: The pH-value of water sample was measured in the laboratory immediately after collection using Bench type (JENWAY, 3410 Electrochemistry Analyzer pH-meter). Dissolved oxygen (BOD: It was determined by a modified Winkler's method [20].…”

Section: Analytical Methods 1) Heavy Metals In Seawatermentioning

confidence: 99%

Impact of Selected Environmental Pollutants on the Ultrastructure of the Gills in <i>Pinctada radiata</i> from Coastal Zones, Egypt

Hamed¹,

Radwan²,

Saad³

2014

OJE

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There has been an increasing interest in marine oysters (order: Petridae) in recent years because their numbers are declining in many parts of the world and also because they are used as monitors of pollution. The present study describes the microscopic structure of gills as viewed by light and electron microscopy in two locations selected in Alexandria coast, Eastern Harbor (E.H.) and El Asafra. The specimens in the E.H. represent the presence of extracellular mineralized granules.

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“…[16] and [19] agreed on that the temperature of the ambient water influences the metabolic rate of the organisms and hence the uptake and release of contaminants. Temperature may affect the growth, distribution of aquatic organisms.…”

Section: Resultsmentioning

confidence: 84%

“…Temperature may affect the growth, distribution of aquatic organisms. [19] and [20] reported that in temperature climates, oysters start to accumulate glycogen before winter and that, metal accumulation in the oyster soft tissues has little toxic effect on overall metabolism. [21] recorded pH-values of water at Abu-Quir site as (7.57 -8.26).…”

Section: Resultsmentioning

confidence: 99%

Temporal and Spatial Effects on Some Physiological Parameters of the Bivalve <i>Lithophaga lithophaga</i> (Linnaeus, 1758) from Coastal Regions of Alexandria, Egypt

Radwan¹,

Hamed²,

Saad³

2014

OJE

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High Zn and Cd accumulation in the oyster Crassostrea rhizophorae, and its relevance as a sentinel species

Cited by 29 publications

References 14 publications

A preliminary integrated genetic map distinguishes every chromosome pair and locates essential genes related to abiotic adaptation of Crassostrea angulata/gigas

A preliminary integrated genetic map distinguishes every chromosome pair and locates essential genes related to abiotic adaptation of Crassostrea angulata/gigas

Impact of Selected Environmental Pollutants on the Ultrastructure of the Gills in <i>Pinctada radiata</i> from Coastal Zones, Egypt

Temporal and Spatial Effects on Some Physiological Parameters of the Bivalve <i>Lithophaga lithophaga</i> (Linnaeus, 1758) from Coastal Regions of Alexandria, Egypt

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High Zn and Cd accumulation in the oyster Crassostrea rhizophorae, and its relevance as a sentinel species

Cited by 29 publications

References 14 publications

A preliminary integrated genetic map distinguishes every chromosome pair and locates essential genes related to abiotic adaptation of Crassostrea angulata/gigas

A preliminary integrated genetic map distinguishes every chromosome pair and locates essential genes related to abiotic adaptation of Crassostrea angulata/gigas

Impact of Selected Environmental Pollutants on the Ultrastructure of the Gills in &lt;i&gt;Pinctada radiata&lt;/i&gt; from Coastal Zones, Egypt

Temporal and Spatial Effects on Some Physiological Parameters of the Bivalve &lt;i&gt;Lithophaga lithophaga&lt;/i&gt; (Linnaeus, 1758) from Coastal Regions of Alexandria, Egypt

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Impact of Selected Environmental Pollutants on the Ultrastructure of the Gills in <i>Pinctada radiata</i> from Coastal Zones, Egypt

Temporal and Spatial Effects on Some Physiological Parameters of the Bivalve <i>Lithophaga lithophaga</i> (Linnaeus, 1758) from Coastal Regions of Alexandria, Egypt