Impact of Selected Environmental Pollutants on the Ultrastructure of  the Gills in &amp;lt;i&amp;gt;Pinctada radiata&amp;lt;/i&amp;gt; from Coastal Zones, Egypt

Hamed, Sherifa S.; Radwan, Eman; Saad, Gaber Ahmed

doi:10.4236/oje.2014.414076

Cited by 3 publications

(4 citation statements)

References 36 publications

(51 reference statements)

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“…Effects of environmental stressors on immunological function in P. imbricata have been elucidated by Kuchel, Raftos, and Nair (2010), which demonstrated as fluctuations in environmental conditions affect circulating haemocytes and their cytochemistry. Such results are confirmed and implemented by the present investigation, in agreement with the hypothesis that physiological responses to environmental stresses in the P. imbricata specie complex, especially at the haemocyte level, may be valuable tools in monitoring programs, eventually coupled with other proposed targets, as gill ultrastructure (Hamed et al, 2014). Such an option, which has been recently proposed for another Pteriomorphia, that is, the Mediterranean endemic Spondylus gaederopus L. 1758 (Fabrello et al, 2022), for Pinctada ssp.…”

Section: Discussionsupporting

confidence: 91%

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

Pagano

Fabrello

Multisanti

et al. 2022

Microscopy Res & Technique

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The pearl oyster Pinctada imbricata radiata (Leach, 1814), from the Pacific Ocean, was one of the first species to reach via Suez the Mediterranean, colonizing the eastern basin and recently spreading to the western. The species showed to be able to adapt to a wide range of climatic, hydrological, and ecological conditions. Since 2000 it reached the Strait of Messina, where is now infesting the transitional waters of the oriented natural reserve “Laguna di Capo Peloro.” Due to such resistance and adaptation ability, various assays were performed. Haemocyte morpho‐functional aspects were evaluated in haemolymph samples fixed with 1% and 2% glutaraldehyde for optical and electron microscopy (TEM). The following assays were carried out: cell characterization using several dyes, detection of intra‐ and extracellular lipids, the capability of phagocytosis using the yeast Saccharomyces cerevisiae and to produce superoxide anion (O2−). Detection of several enzymes, such as acid and alkaline phosphatase, arylsulfatase, chloro‐acetylesterase and β‐glucuronidase was also assessed. Cell count was demonstrated to be abundant with a mean of 8.263 × 106 mm2 ± 0.935 × 106 (SD). Two main cell populations were noticed: granulocytes and hyalocytes, both competent for phagocytosis, to produce O2−, and characterized by lipids. Based on the granule analysis, enzymatic activity was also demonstrated. The observations under TEM confirmed all the results obtained. This study supports the hypothesis that P. imbricata radiata can be usefully employed as a model organism in environmental biomonitoring. Moreover, since the species represent potential threats to native species and ecosystems, further insights into its biological adaptations in invaded ecosystems are recommended.

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Section: Discussionsupporting

confidence: 91%

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

Pagano

Fabrello

Multisanti

et al. 2022

Microscopy Res & Technique

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“…Ultrastructure analysis performed at the Transmission Electron Microscopy Laboratory, Eijkman Institute for Molecular Biology (EIMB), Indonesia. Samples preparation for TEM analysis is carried out referring to the Hamed et al (2014) methods. Samples were fixed in 2.5% glutaraldehyde solution (pH 7.2, buffered 0.1 M phosphate buffer) for 2-4 h at 4 o C and rinsed in 0.1 M phosphate buffer and then post-fixed in 1% osmium tetroxide (OsO4) solution for 2 h at 4 o C. After fixation, the samples were washed with 0.1 M phosphate buffer 4 times for 2 h and dryed with ascending grades of ethanol.…”

Section: Ultrastructure Analysis In Gillsmentioning

confidence: 99%

“…Currently study have revealed that bivalve can retain and perhaps accumulate heavy metals in their soft tissues, included in gills (Hamed et al, 2014). The primary organs for metal accumulation are gills, which also have the highest amounts of pollution.…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of Pb in the Gills of Perna viridis (Bivalvia: Mytilidae) in Jakarta Bay, Indonesia and its Histological Alteration

Kunda,

Rumanta,

Hutasoit

2024

jppipa, pendidikan ipa, fisika, biologi, kimia

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Pollution in Jakarta Bay associated with terrestrial run-off and eutrophication. Bivalves have been recognized as aquatic organisms that play the most dominant role in aquatic ecosystems. Pb is an element that in many cases causes health problems in the liver, kidneys, hematopoietic system and nervous system. This study aims to analyze the bioaccumulation of Pb in the gills of P. viridis in Jakarta Bay, and its histological changes. Bioaccumulation analysis using the AAS and histology changes using transmission electron microscopy (TEM) methods. The results of AAS, showed that the value of Pb concentration in gill tissue at the two seasons did not show a noticeable difference. Pb concentrations in gills were lower at 1, 4, and 5 than at 2 and 3 stations. The results of ultrastructure analysis as an impact of Pb accumulation showed that thickening of the nucleus membrane, and a decrease in the number of microvilli. The thickening of the nucleus membrane, and the decrease in the number of microvilli are cellular responses to the entry of the Pb heavy metal into the cell. Besides, the results of this study showed inflammation, a decrease in the number of mitochondria, as well as causing cristae to be empty.

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“…They can be used in detecting and monitoring the recent environment in terms of pollution, therefore mollusks (bivalves and gastropods) are the cornerstone of this study. In most recent researches, there are several contributions towards the geochemical analysis using invertebrate shells (Hamed et al, 2014). Near-shore human activities may result in marine organisms being exposed to high concentrations of metals (Esslemont, 2000;El-Sorogy, 2008;Oladoja et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Pollution impact on recent mollusks along the Mediterranean coast between Abu Traba and Al Kuwifia, NE Libya

M. Bago,

M. Muftah,

R. Shaltami

et al. 2022

LJST

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The current study assessed the levels of pollution on recent mollusks along the Mediterranean coast from Abu Traba to Al Kuwifia, Northeast Libya. Twenty-five molluskan species have been documented in total. However, only thirteen taxa were chemically analyzed in this study. The chemical analysis showed that the Cerastoderma glacum and Venus sp. are good accumulators for Zinc (Zn), Lead (Pb) and Thorium (Th), while Cerithium sp. A and Cerithium sp. B are fine collectors for Uranium (U). Most of the analyzed metals have Enrichment Factor (EF)>2 suggesting that they are mainly of anthropogenic origin. We recommend treating the pollution levels of Lead (Pb), Arsenic (As) and Selenium (Se) in different localities of the study area, especially in Abu Traba and Al Kuwifia localities.

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Impact of Selected Environmental Pollutants on the Ultrastructure of the Gills in <i>Pinctada radiata</i> from Coastal Zones, Egypt

Cited by 3 publications

References 36 publications

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

Bioaccumulation of Pb in the Gills of Perna viridis (Bivalvia: Mytilidae) in Jakarta Bay, Indonesia and its Histological Alteration

Pollution impact on recent mollusks along the Mediterranean coast between Abu Traba and Al Kuwifia, NE Libya

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