High-yield paper-based quantitative blood separation system

Lu, Zhengda; Rey, Elizabeth; Vemulapati, Sasank; Srinivasan, Balaji; Mehta, Saurabh; Erickson, David

doi:10.1039/c8lc00717a

Cited by 39 publications

(56 citation statements)

References 22 publications

(25 reference statements)

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“…Test strips were imaged by a portable imaging device similar to one previously described by Lu et al 21 . Briefly, the device consists of a 5 megapixel 1080p HD CMOS camera (Raspberry Pi, Cambridge, UK), focusing lens and LEDs (Thorlabs Inc., Newton, NJ, USA), Raspberry Pi computer board, rechargeable lithium ion battery pack (Adafruit Industries, New York, NY, USA), and cassette tray, all surrounded by a 3D-printed light-tight container.…”

Section: Methodsmentioning

confidence: 99%

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

et al. 2017

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The quantification of analyte concentrations using lateral flow assays is a low-cost and user-friendly alternative to traditional lab-based assays. However, sandwich-type immunoassays are often limited by the high-dose hook effect, which causes falsely low results when analytes are present in very high concentrations. In this paper, we present a reaction kinetics-based technique that solves this problem, significantly increasing the dynamic range of these devices. With the use of a traditional sandwich lateral flow immunoassay, a portable imaging device, and a mobile interface, we demonstrate the technique by quantifying C-reactive protein concentrations in human serum over a large portion of the physiological range. The technique could be applied to any hook effect-limited sandwich lateral flow assay and has a high level of accuracy even in the hook effect range.

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Section: Methodsmentioning

confidence: 99%

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

et al. 2017

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“…developed a paper-based system which can detect ferritin from full blood using a series of papers with different pore sizes. The authors found the methodology comparable in quality to standard centrifugation for blood separation [123].…”

Section: Challenges Of Paper-based Assaysmentioning

confidence: 99%

Designing Paper-Based Immunoassays for Biomedical Applications

Hristov

Rodriguez-Quijada

Gómez-Márquez

et al. 2019

Sensors

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Paper-based sensors and assays have been highly attractive for numerous biological applications, including rapid diagnostics and assays for disease detection, food safety, and clinical care. In particular, the paper immunoassay has helped drive many applications in global health due to its low cost and simplicity of operation. This review is aimed at examining the fundamentals of the technology, as well as different implementations of paper-based assays and discuss novel strategies for improving their sensitivity, performance, or enabling new capabilities. These innovations can be categorized into using unique nanoparticle materials and structures for detection via different techniques, novel biological species for recognizing biomarkers, or innovative device design and/or architecture.

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“…At present, several ICT kits are available for assaying analytes in WBS. Most of these kits use a plasma-separation membrane for trapping hematocytes and only plasma migrates into a test strip membrane [ 23 , 39 , 40 , 41 ]. However, in our preliminary experiments, the plasma-separation membrane (Cytosep 1660) did not work for trapping hematocytes as expected.…”

Section: Discussionmentioning

confidence: 99%

Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis

et al. 2021

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Human gnathostomiasis is a harmful food-borne zoonosis caused by roundworms of the genus Gnathostoma. The parasite can occasionally migrate to the central nervous system, causing life-threatening disease and death. Here, we report a new point-of-care (POC) test kit, the gnathostomiasis blood immunochromatographic test (GB-ICT) kit. The kit is based on recombinant Gnathostoma spinigerum antigen and detects specific IgG4 antibody in whole-blood samples (WBSs). The GB-ICT kit showed potentially high diagnostic values with simulated WBSs (n = 248), which were obtained by spiking patients’ sera with red blood cells. The accuracy, sensitivity, specificity, and positive and negative predictive values were 95.2%, 100%, 93.8%, 81.5%, and 100%, respectively. Ten WBSs from clinically suspected gnathostomiasis patients were all positive according to the GB-ICT kit, while 10 WBSs from healthy volunteers were negative. The GB-ICT kit is a simple and convenient POC testing tool using finger-prick blood samples: venous blood sampling and serum separation processes are not required. The GB-ICT kit can support clinical diagnosis in remote areas and field settings without sophisticated equipment facilities.

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High-yield paper-based quantitative blood separation system

Cited by 39 publications

References 22 publications

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

Designing Paper-Based Immunoassays for Biomedical Applications

Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis

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