High xylanase production by Trichoderma viride using pineapple peel as substrate and its apllication in pulp biobleaching

Abstract

Xylanases are hydrolases which depolymerize the xylan components present in plants cell wall. Commercial applications for these enzymes include its use in the pulp bleaching, food and animal feed industries, among others. Recently, there is a great interest on the exploitation of agro-industrial wastes as low-cost raw materials for value-added compounds production, as xylanolytic enzymes for industrial applications. This is the first report about the xylanase production using pineapple peel as substrate. The xylanase production by Trichoderma viride was optimized and the enzymes produced were biochemically characterized. Additionally, the effect of these enzymes on pulp biobleaching process was evaluated. High xylanase production was obtained with pineapple peel at 2% concentration, for seven days, in stationary cultivation at 28°C, in pH 7.5. Xylanases were more active at 50°C, pH 6.0-6.5 remaining stable at pH 5.0-5.5. T. viride xylanase was stable at 40°C, showing the half-life (T 1/2) value of 255 min. The enzyme was remarkably stimulated by Mg 2+ and Zn 2+ , while Pb 3+ and Hg 2+ were strong inhibitors of the xylanase activity. This work shows the ability of the filamentous fungus T. viride to produce high levels of xylanases using pineapple peel as substrate, an inexpensive and abundant agroindustrial waste. Therefore, the reduction on kappa number achieved in this investigation revealed the application potential of these enzymes in biobleaching process.

Thermostable xylanase from thermophilic fungi: Biochemical properties and industrial applications

“…For instance, the cellulase-free xylanase from Trichoderma viride was used on kraft pulp from Eucalyptus grandis, resulting in a decrease in its Kappa number and its maintenance of viscosity compared to those of the control, with parameters (enzyme dose, time, temperature, and pH). The results indicated that the enzyme showed potential for use in the pulp and paper industry (Fortkamp and Knob, 2014). Similar studies by Guimarães et al (2013) using a xylanase from Aspergillus aculeatus var aculeatus in the pretreatment of E. grandis pulp, and by Silva et al (2016) described the effective use of xylanase from Penicillium crustosum to bleach the kraft pulp of E. grandis, obtained a significant reduction in Kappa number (5.27 points) corresponding to a 35.04% Kappa efficiency.…”

Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis

“…Interestingly, Zn 2+ increases its α -helix content from 45.3 to 46.8%. Moreover, Zn 2+ has also been reported to be the activator of several enzymes, including carbonyl reductase [47], xylanase [48], nattokinase [49] and lipase [50]. The tendency for a difference between Zn 2+ and other metal ions may be due to the different structural compatibility of metal ions to bind with YlER.

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Application of an endo-xylanase from Aspergillus japonicus in the fruit juice clarification and fruit peel waste hydrolysis

“…Xylan is a major structural component of plant cell walls and its degradation requires the action of several enzymes, among which endoxylanases (EC 3.2.1.8) play a key role (Polizeli et al, 2005;Fortkamp and Knob, 2014). Xylanases occur widely in bacteria, yeasts and fungi.…”