High wavenumber Raman spectroscopy in the characterization of urinary metabolites of normal subjects, oral premalignant and malignant patients

Brindha, Elumalai; Rajasekaran, R.; Aruna, Prakasarao; Koteeswaran, Dornadula; Ganesan, Singaravelu

doi:10.1016/j.saa.2016.06.048

Cited by 21 publications

(23 citation statements)

References 69 publications

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“…Raman spectroscopy has previously been applied directly to clinical samples such as saliva, urine, blood, and tissues to accurately identify diseased states. For example, researchers have used urine or saliva samples to distinguish between patients with and without oral cancer. Using spatially offset Raman spectroscopy, Keller et al.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

et al. 2019

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The traditional bacterial identification method of growing colonies on agar plates can take several days to weeks to complete depending on the growth rate of the bacteria. Successfully decreasing this analysis time requires cell isolation followed by identification. One way to decrease analysis time is by combining dielectrophoresis (DEP), a common technique used for cell sorting and isolation, and Raman spectroscopy for cell identification. DEP‐Raman devices have been used for bacterial analysis, however, these devices have a number of drawbacks including sample heating, cell‐to‐electrode proximity that limits throughput and separation efficiency, electrode fouling, or inability to address sample debris. Presented here is a contactless DEP‐Raman device to simultaneously isolate and identify particles from a mixed sample while avoiding common drawbacks associated with other DEP designs. Using the device, a mixed sample of bacteria and 3 μm polystyrene spheres were isolated from each other and a Raman spectrum of the trapped bacteria was acquired, indicating the potential for cDEP‐Raman devices to decrease the analysis time of bacteria.

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Section: Introductionmentioning

confidence: 99%

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

et al. 2019

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“…Of all the included articles, 25 [1, studies had data available for extraction for an oral cancer group, 3 studies [46][47][48] had data available for an oral precancerous lesion group, and 13 studies [21,[49][50][51][52][53][54][55][56][57][58][59][60] had data available for both groups. We used histopathological samples as the gold standard for all studies.…”

Section: Description Of Studies Included In the Reviewmentioning

confidence: 99%

“…Among the 38 studies in the oral cancer group, 5 [33,53,54,56,60] studies evaluated in vivo tissue, whereas 33 [1,[21][22][23][24][25][26][27][28][29][30][31][32][34][35][36][37][38][39][40][41][42][43][44][45][49][50][51][52]55,[57][58][59] studies evaluated in vitro samples. in vitro samples can be categorized as tissues (n = 13), [1,21,22,24,26,27,30,39,[43][44][45]50,55] bio fluids (n = 15),…”

Section: Description Of Studies Included In the Reviewmentioning

confidence: 99%

The accuracy of Raman spectroscopy in the detection and diagnosis of oral cancer: A systematic review and meta‐analysis

Zhan

Yuan

et al. 2020

J Raman Spectroscopy

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The aim of this study was to systematically review and assess the diagnostic accuracy of Raman spectroscopy (RS) for oral cancer tissue, oral precancerous lesions, and normal oral tissue. Methods: PubMed, Embase, Web of Science, the China National Knowledge Infrastructure, and gray literature were searched for all relevant articles published before July 2019. We used the Quality Assessment of Diagnostic Accuracy Studies tool to assess the quality of the included studies. We estimated the pooled sensitivity, specificity, positive and negative likelihood ratios (PLR and NLR), diagnostic odds ratio (DOR), and established summary receiver operating characteristic (SROC) curves to identify the diagnostic accuracy of RS for oral cancer tissue, oral precancerous lesions, and normal oral tissue. In addition, the area under the curve (AUC) was reported to estimate the overall effectiveness of RS. Results: A total of 41 articles were eligible for this meta-analysis. The coalescent sensitivity and specificity of RS in diagnosing oral cancer in vivo were 0.91 and 0.85. The positive likelihood ratio, the negative likelihood ratio, and the area under the curve were 8.01, 0.10, and 0.9284. The frozen tissue subgroup in vitro oral cancer group showed improved diagnostic accuracy with an AUC of 0.9968. The in vitro frozen tissue group also showed better diagnostic accuracy in distinguishing between oral precancerous lesions and normal oral tissues. Conclusions: RS has the advantages of being noninvasive and able to provide real-time and in situ results, so it deserves to be studied and improved further to better serve clinical work. K E Y W O R D S diagnosis, meta-analysis, oral cancer, oral precancerous lesion, Raman spectroscopy 1 | INTRODUCTION Oral cancer, a type of head and neck cancer, is a generic term for malignant tumors that occur in the mouth, most † These authors contributed equally to this work.

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“…A neutralization reaction was carried out with piperinic acid 2 in an ethanolic solution of potassium hydroxide, thereby obtaining the potassium piperinoate 3 in high yield. The 2-chloro- N -(4-nitrophenyl)acetamide 5 compound was obtained in N -acetylation reaction between 4-nitroaniline 4 and 2-chloroacetyl chloride [ 2 ]. Reaction between the potassium salt of piperinic acid 3 with 2-chloro- N -(4-nitrophenyl)acetamide 5 in N , N -Dimethylformamide (DMF) provided the formation of N -(p-nitrophenyl)acetamide piperinoate HE-02 in yield of 60%.…”

Section: Figures Scheme and Tablesmentioning

confidence: 99%

“…Cancer is a generic term that refers to a set of diseases characterized by the presence of cells in continuous proliferation with invasion and metastasis properties [ 1 ]. It is one of the most common causes of high morbidity and mortality [ 2 ] which is an important public health problem worldwide [ 3 ]. Tumor cells share several characteristics, including unregulated proliferation, angiogenesis induction, the ability to escape from immunological detection, and tumor-promoting inflammation [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Th1-Biased Immunomodulation and In Vivo Antitumor Effect of a Novel Piperine Analogue

Santos

Brito

Ferreira

et al. 2018

IJMS

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Natural products have an important role as prototypes in the synthesis of new anticancer drugs. Piperine is an alkaloid amide with antitumor activity and significant toxicity. Then, the N-(p-nitrophenyl)acetamide piperinoate (HE-02) was synthesized, and tested for toxicological and antitumor effects. The toxicity was evaluated in vitro (on RAW 264.7 cells and mice erythrocytes) and in vivo (acute toxicity in mice). The Ehrlich ascites carcinoma model was used to evaluate the antitumor activity of HE-02 (6.25, 12.5 or 25 mg/kg, intraperitoneally, i.p.), as well as toxicity. HE-02 induced only 5.01% of hemolysis, and reduced the viability of RAW 264.7 cells by 49.75% at 1000 µg/mL. LD50 (lethal dose 50%) was estimated at around 2000 mg/kg (i.p.). HE-02 reduced Ehrlich tumor cell viability and peritumoral microvessels density. There was an increase of Th1 helper T lymphocytes cytokine profile levels (IL-1β, TNF-α, IL-12) and a decrease of Th2 cytokine profile (IL-4, IL-10). Moreover, an increase was observed on reactive oxygen species and nitric oxide production. Weak in vivo toxicological effects were recorded. Our data provide evidence that the piperine analogue HE-02 present low toxicity, and its antitumor effect involves modulation of immune system to a cytotoxic Th1 profile.

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High wavenumber Raman spectroscopy in the characterization of urinary metabolites of normal subjects, oral premalignant and malignant patients

Cited by 21 publications

References 69 publications

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

The accuracy of Raman spectroscopy in the detection and diagnosis of oral cancer: A systematic review and meta‐analysis

Th1-Biased Immunomodulation and In Vivo Antitumor Effect of a Novel Piperine Analogue

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