High-virulence CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from commercial turkeys

Silva, Ketrin Cristina da; Cunha, Marcos Paulo Vieira; Cerdeira, Louise; Oliveira, Maria Gabriela Xavier de; Oliveira, M. C.; Gomes, C. B.; Lincopán, Nilton; Knöbl, Terezinha; Moreno, Andréa Micke

doi:10.1016/j.diagmicrobio.2016.10.001

Cited by 13 publications

(13 citation statements)

References 21 publications

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“…The results from the present study, suggest that transfer of bla CMY−2 -IncI1/ST12 plasmids between E. coli and Salmonella is also likely to occur, and could be considered a driver of the frequent occurrence of this resistance gene along the poultry chain. This particular gene-plasmid association has been described in E. coli from Brazil (da Silva et al, 2017 ), Salmonella from the USA (Folster et al, 2010 ) and E. coli and Salmonella from Europe (Accogli et al, 2013 ; Smith et al, 2015 ), suggesting an epidemiological link between the presence of bla CMY−2 -IncI1/ST12 and poultry from different countries. Nevertheless, WGS of these plasmids is necessary to assess the level of genetic relatedness among them and estimate the potential transmission of these plasmids between E. coli and Salmonella .…”

Section: Discussionmentioning

confidence: 78%

Genomic Characterization of Extended-Spectrum Cephalosporin-Resistant Salmonella enterica in the Colombian Poultry Chain

et al. 2018

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Salmonella enterica serovars have been isolated from Colombian broilers and broiler meat. The aim of this study was to investigate the diversity of ESBL/pAmpC genes in extended-spectrum cephalosporin resistant Salmonella enterica and the phylogeny of ESBL/pAmpC-carrying Salmonella using Whole Genome Sequencing (WGS). A total of 260 cefotaxime resistant Salmonella isolates, obtained between 2008 and 2013 from broiler farms, slaughterhouses and retail, were included. Isolates were screened by PCR for ESBL/pAmpC genes. Gene and plasmid subtyping and strain Multi Locus Sequence Typing was performed in silico for a selection of fully sequenced isolates. Core-genome-based analyses were performed per ST encountered. blaCMY−2−like was carried in 168 isolates, 52 carried blaCTX−M−2 group, 7 blaSHV, 5 a combination of blaCMY−2−like-blaSHV and 3 a combination of blaCMY−2−like-blaCTX−M−2 group. In 25 isolates no ESBL/pAmpC genes that were screened for were found. WGS characterization of 36 selected strains showed plasmid-encoded blaCMY−2 in 21, blaCTX−M−165 in 11 and blaSHV−12 in 7 strains. These genes were mostly carried on IncI1/ST12, IncQ1, and IncI1/ST231 plasmids, respectively. Finally, 17 strains belonged to S. Heidelberg ST15, 16 to S. Paratyphi B variant Java ST28, 1 to S. Enteritidis ST11, 1 to S. Kentucky ST152 and 1 to S. Albany ST292. Phylogenetic comparisons with publicly available genomes showed separate clustering of Colombian S. Heidelberg and S. Paratyphi B var. Java. In conclusion, resistance to extended-spectrum cephalosporins in Salmonella from Colombian poultry is mainly encoded by blaCMY−2 and blaCTX−M−165 genes. These genes are mostly associated with IncI1/ST12 and IncQ1 plasmids, respectively. Evolutionary divergence is observed between Colombian S. Heidelberg and S. Paratyphi B var. Java and those from other countries.

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Section: Discussionmentioning

confidence: 78%

Genomic Characterization of Extended-Spectrum Cephalosporin-Resistant Salmonella enterica in the Colombian Poultry Chain

et al. 2018

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“…In contrast to the IncK2 plasmids, we detected more diverse IncI1 plasmid structures. IncI1 plasmids carrying bla CMY-2 were reported worldwide and differ considerably in their plasmid backbone structures [ 7 , 18 , 61 , 62 ]. In the present study most IncI1 plasmids could be assigned to pST12 (39/61) and pST2 (12/61).…”

Section: Discussionmentioning

confidence: 99%

Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany

et al. 2018

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BackgroundResistance to 3rd-generation cephalosporins in Escherichia coli is mostly mediated by extended-spectrum beta-lactamases (ESBLs) or AmpC beta-lactamases. Besides overexpression of the species-specific chromosomal ampC gene, acquisition of plasmid-encoded ampC genes, e.g. blaCMY-2, has been described worldwide in E. coli from humans and animals. To investigate a possible transmission of blaCMY-2 along the food production chain, we conducted a next-generation sequencing (NGS)-based analysis of 164 CMY-2-producing E. coli isolates from humans, livestock animals and foodstuff from Germany.ResultsThe data of the 164 sequenced isolates revealed 59 different sequence types (STs); the most prevalent ones were ST38 (n = 19), ST131 (n = 16) and ST117 (n = 13). Two STs were present in all reservoirs: ST131 (human n = 8; food n = 2; animal n = 6) and ST38 (human n = 3; animal n = 9; food n = 7). All but one CMY-2-producing ST131 isolates belonged to the clade B (fimH22) that differed substantially from the worldwide dominant CTX-M-15-producing clonal lineage ST131-O25b clade C (fimH30). Plasmid replicon types IncI1 (n = 61) and IncK (n = 72) were identified for the majority of blaCMY-2-carrying plasmids. Plasmid sequence comparisons showed a remarkable sequence identity, especially for IncK plasmids. Associations of replicon types and distinct STs were shown for IncK and ST57, ST429 and ST38 as well as for IncI1 and ST58. Additional β-lactamase genes (blaTEM, blaCTX-M, blaOXA, blaSHV) were detected in 50% of the isolates, and twelve E. coli from chicken and retail chicken meat carried the colistin resistance gene mcr-1.ConclusionWe found isolates of distinct E. coli clonal lineages (ST131 and ST38) in all three reservoirs. However, a direct clonal relationship of isolates from food animals and humans was only noticeable for a few cases. The CMY-2-producing E. coli-ST131 represents a clonal lineage different from the CTX-M-15-producing ST131-O25b cluster. Apart from the STdriven spread, plasmid-mediated spread, especially via IncI1 and IncK plasmids, likely plays an important role for emergence and transmission of blaCMY-2 between animals and humans.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4976-3) contains supplementary material, which is available to authorized users.

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“…However, since 2015, studies have found pAmpC-producing bacteria in food-producing animals, mainly chicken meat in Brazil, leading to the hypothesis that this might become an infection source or reservoir that contributes to the spread of these bacteria. The bla CMY-2 gene is also the pAmpC variant more frequently found in food-producing animals in Brazil [12–16, 30]. Studies have detected a high prevalence of bla CMY-2 genes harbored on different plasmids in E. coli from poultry [13, 14].…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, pAmpC betalactamases were first reported in strains isolated from humans between 2007 and 2008 [10, 11]. Since then, AmpC-producing bacteria have been reported in food-producing animals, such as poultry carcasses [12–16]. However, there are few studies on AmpC-mediated resistance in human clinical and veterinary medicine in Brazil.…”

Section: Introductionmentioning

confidence: 99%

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

et al. 2019

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Background Food-producing animals, mainly poultry, have been associated with the maintenance and dissemination of antibiotic-resistant bacteria, such as plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae, to humans, thus impacting food safety. Many studies have shown that Escherichia coli strains isolated from poultry and humans infections share identical cephalosporin resistance, suggesting that transmission of resistance from poultry meat to humans may occur. The aim of this study was to characterize pAmpC-producing E. coli strains isolated from chicken carcasses and human infection in a restrict area and to determine their antimicrobial resistance profiles, and molecular type by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Results A total of 14 pAmpC-producing E. coli strains were isolated, including eight strains from chicken carcasses and six strains from human infections (from urine, tissue and secretion). The bla CMY-2 gene was identified in all pAmpC-producing E. coli strains by polymerase chain reaction (PCR) and DNA sequencing. High percentages of strains resistant to tetracycline, nalidixic acid and sulfamethoxazole-trimethoprim (78–92%) were detected, all of which were considered multidrug-resistant. Among the non-beta-lactam resistance genes, the majority of the strains showed tet A, tet B, sul I and sul II. No strain was considered an extended-spectrum beta-lactamases (ESBL) producer, and the bla TEM-1 gene was found in 2 strains isolated from human infection. Six strains from chicken carcasses and four strains from humans infections were linked to an ISE cp1 -like element. Through MLST, 11 sequence types were found. Three strains isolated from human infection and one strain isolated from chicken carcasses belonged to the same sequence type (ST354). However, considerable heterogeneity between the strains from chicken carcasses and humans was confirmed by PFGE analysis. Conclusion This study showed the prevalence of E. coli strains producing bla CMY-2 linked to ISE cp1 that were present in both chickens and humans in a restricted area. Our results also suggest the presence of a highly diverse strains that harbor pAmpC, indicating no clonal dissemination. Therefore, continuous monitoring and comparative analyses of resistant bacteria from humans and food-producing animals are needed.

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High-virulence CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from commercial turkeys

Cited by 13 publications

References 21 publications

Genomic Characterization of Extended-Spectrum Cephalosporin-Resistant Salmonella enterica in the Colombian Poultry Chain

Genomic Characterization of Extended-Spectrum Cephalosporin-Resistant Salmonella enterica in the Colombian Poultry Chain

Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany

Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

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