High Vero Cell Density and Rabies Virus Proliferation on Fibracel Disks Versus Cytodex-1 in Spinner Flask

Hassanzadeh, Seyed Mehdi; Zavareh, Ali; Shokrgozar, Mohammad Ali; Ramezani, Ali; Fayaz, Ahmad

doi:10.3923/pjbs.2011.441.448

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…Infection of the BHK21 cell line showed 100% positivity of infected mice brain suspension resulting in specific CPE of RABV by the second day postinfection as cell rounding followed by cell lysis and detachment of the cell sheet. These results match what was described by Consales et al (1990) , Knezevic (2008) , and Hassanzadeh et al (2011) , who found that RABV could be harvested from BHK cell culture with similar findings at around 48 hours of infection.…”

Section: Discussionsupporting

confidence: 91%

Comparative evaluation of some techniques used for detection of rabies virus

Hegazy,

Hosseny,

Senna

et al. 2023

Open Vet J

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Background: Neurotropic viruses in the family Rhabdoviridae, genus Lyssavirus, are what cause rabies, an acute, progressive, and highly lethal encephalomyelitis. Aim: Evaluation of the used diagnostic techniques in order to determine the most simple; rapid and accurate test for rabies virus recognition in different specimens aiming to reach rapid diagnosis as a step aid in the disease control and to prevent or even minimize the suspected hazard. Method: The used techniques included infection trial of Swiss mice with the mice adapted rabies virus (CVS) followed by detection of the virus in infected mice brain. Virus detection was carried out through application BHK21 cell line infection; Fluorescent antibody technique FAT; Latex agglutination test LAT; direct ELISA; Rabies antigen detection kit ELISA; conventional PCR Results: It was found that virus inoculation in mice and BHK21 cell line need 5-7 days with positivity 90 and 100% respectively. Rapid antigen kit was able to detect rabies antigen in mice brains suspension and BHK21 infected fluid within 3-5 minutes with the percentage of 60 and 55.5 respectively. In 1-1.5 hours, the direct fluorescent antibody method (DFAT) detected 90% and 100% of the rabies antigen in BHK21 cell line infection and brain impressions, respectively. Latex agglutination showed clear results with 88.8% with BHK21 infected fluid within 3-5 minutes while it did not carry out on brain emulsions to prevent falsely positive results brought on by the presence of tissue fragments. Conventional one step polymerase chain reaction (PCR) revealed 100% positivity with either brain or cell culture preparations within 2 days. Direct enzyme immune linked sorbent assay (ELISA) showed 88.8% positivity with BHK21 infected fluid with one day work. Conclusion: Mice inoculation test, cell culture infection; DFAT and PCR are the most accurate techniques for detection of rabies virus with positivity 90-100% followed by LAT and ELISA with positivity 88.8% and lastly Rabies antigen ELISA kit (RAK) with positivity 55.5-60% taking in consideration the required time for each. In addition, the positivity % of the applied tests revealed their sensitivity and specificity.

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Section: Discussionsupporting

confidence: 91%

Comparative evaluation of some techniques used for detection of rabies virus

Hegazy,

Hosseny,

Senna

et al. 2023

Open Vet J

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“…Also, transmission can be via the saliva that is the major source of virus spread ( 5 ). Rabies disease is endemic in the wild life of our country, therefore domestic animals are frequently infected while, enhancing cases of animal bite and scattering of disease virus in most provinces of Iran, declare a need to pay more attention to controlling this disease ( 8 ).…”

Section: Discussionmentioning

confidence: 99%

Severe Abdominal Pain as the First Manifestation of Rabies

Ayatollahi

Sharifi

Shahcheraghi

2014

Jundishapur J Microbiol

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Introduction:Rabies is an acute fatal viral disease that is generally transmitted from animals to humans following wild and domestic animal bites. The rabies virus enters the body from the area where the individual is bitten, and then the virus moves towards the brain and involves the nerves.Case Presentation:During the years 2001-2011, there have been 73 reported rabies cases. About 50,000 reported human deaths are annually due to rabies. The actual number of human deaths due to rabies in Asia especially India, Pakistan and Bangladesh are more than these numbers, since there is no advanced surveillance system for disease control to determine the actual number of infected and fatal human cases. According to the World Health Organization (WHO) reports, more than 10 million people who are bitten by animals are annually treated by prophylactic treatment regimens for rabies, worldwide.Conclusions:This paper reports on a case of human rabies with the first disease manifestation (severe abdominal pain). The patient reported extensive biting on his left leg by a dog. He had a slight fever of 38.1°C. It has been recommended that a careful history should be taken from patients for diagnosis of rabies disease. A complete history should be taken from patients for diagnosis of disease, because rabies could be wrong with various diseases with atypical symptoms. because various diseases with atypical symptoms or long incubation periods can visit.

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“…On the one hand, in case of very fragile cells, in particular cells infected by viruses for virus production, it was shown that the replacement of solid carriers such as Cytodex 1 by Fibra-cel discs, in which the cells are immobilized, led to higher (rabies) virus production probably because of reduced cell loss caused by bead collisions [39]. On the other hand, cells can also be protected from turbulences at high agitation by addition of thickening agents (polymers to increase viscosity) [40].…”

Section: (I) Microcarrier Technology: Basicsmentioning

confidence: 99%

Advances in cell culture: anchorage dependence

Merten

2015

Phil. Trans. R. Soc. B

129

103

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Anchorage-dependent cells are of great interest for various biotechnological applications. (i) They represent a formidable production means of viruses for vaccination purposes at very large scales (in 1000-6000 l reactors) using microcarriers, and in the last decade many more novel viral vaccines have been developed using this production technology. (ii) With the advent of stem cells and their use/potential use in clinics for cell therapy and regenerative medicine purposes, the development of novel culture devices and technologies for adherent cells has accelerated greatly with a view to the large-scale expansion of these cells. Presently, the really scalable systems-microcarrier/ microcarrier-clump cultures using stirred-tank reactors-for the expansion of stem cells are still in their infancy. Only laboratory scale reactors of maximally 2.5 l working volume have been evaluated because thorough knowledge and basic understanding of critical issues with respect to cell expansion while retaining pluripotency and differentiation potential, and the impact of the culture environment on stem cell fate, etc., are still lacking and require further studies. This article gives an overview on critical issues common to all cell culture systems for adherent cells as well as specifics for different types of stem cells in view of small-and large-scale cell expansion and production processes.

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High Vero Cell Density and Rabies Virus Proliferation on Fibracel Disks Versus Cytodex-1 in Spinner Flask

Cited by 9 publications

References 17 publications

Comparative evaluation of some techniques used for detection of rabies virus

Comparative evaluation of some techniques used for detection of rabies virus

Severe Abdominal Pain as the First Manifestation of Rabies

Advances in cell culture: anchorage dependence

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