High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assays

Concordance Study Between the Investigator Argus Y-12 QS and the AmpFISTR® YfilerTM Kits in a Brazilian Population Sample

“…One sample was typed at the DYS385 locus as 14-17 using the Yfiler™ kit and as 14-16.3 when using Argus Y-12 ( Figure 1). This difference has been reported previously [4] and is also mentioned in the kit´s manual that allele 16.3…”

Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samples

“…In addition, a concordance study with a beta-test version of Applied Biosystems' Yfiler kit found that there was complete agreement with the Schoske et al [7] data with the exception of a nomenclature difference in DYS448. The nomenclature by Redd et al [8] for DYS448 is now recommended and part of the certified values for the NIST Human Y-chromosome DNA Profiling Standard Reference Material SRM 2395 (see http://ts.nist.gov/ts/htdocs/230/ 232/232.htm, then enter 2395).…”

“…Results and discussion: This data set provides a continuation of information made available on these same samples by Schoske et al [7], where results from 27 amplified regions of the Y-chromosome were reported. In addition, a concordance study with a beta-test version of Applied Biosystems' Yfiler kit found that there was complete agreement with the Schoske et al [7] data with the exception of a nomenclature difference in DYS448.…”

The case of the unreliable SNP: Recurrent back-mutation of Y-chromosomal marker P25 through gene conversion

“…P25 is not the only widely used marker within the palindromic repeats of Yq: the Y-STR DYS464 [21][22][23][24] lies in the 'r' type repeats of the AZFc region [13], and is present in four copies in the database sequence. It may owe its high diversity to gene conversion between copies, as well as to conventional slippage mutation processes.…”