Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samples

Butler, John M.; Decker, Amy E.; Vallone, Peter M.; Kline, Margaret C.

doi:10.1016/j.forsciint.2005.02.011

Cited by 43 publications

(32 citation statements)

References 13 publications

(19 reference statements)

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“…html). Up to now, reports on large volumes of population genetic data from a wide range of ethnic groups for the Y-STR loci have focused on the core loci [16][17][18][19], while several papers have reported polymorphic Y-STR loci other than the core loci [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci

et al. 2007

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We developed three short amplicon Y-chromosomal short tandem repeat (miniY-STR) polymerase chain reaction multiplex systems for 16 Y-STR loci (DYS441, DYS446, DYS462, DYS481, DYS485, DYS495, DYS505, DYS510, DYS511, DYS549, DYS 575, DYS578, DYS593, DYS618, DYS638, and DYS643), using newly designed primer sets. In an assay of 238 Japanese males using the three miniY-STR systems, amplification product lengths ranged from 91 to 151 bp for all 16 Y-STR loci. We identified 212 different haplotypes among the 238 individuals, finding haplotype diversity and discrimination capacity of 0.9974 and 0.8908, respectively. An assay of degraded DNA samples using the three miniY-STR multiplex systems, including artificially degraded samples and degraded forensic casework samples, proved remarkably effective. In conclusion, analyses of miniY-STR multiplex systems will play an important role in forensic applications involving degraded DNA samples for which genotyping using only commercial kits is ill-suited.

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Section: Introductionmentioning

confidence: 99%

Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci

et al. 2007

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“…Some PCR primers were designed either by using the STRbase DNA database (http:// www.cstl.nist.gov/strbase) or on the basis of previously published literature (Tang et al, 2003;Butler et al, 2006), which was followed by synthesis by Sigma Oligo (Sigma-Aldrich, St. Louis, MO, USA). The Type-it HRM PCR Kit was purchased from Qiagen (Hilden, Germany).…”

Section: Hrm Analysismentioning

confidence: 99%

Y-STR genetic screening by high-resolution melting analysis

et al. 2016

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ABSTRACT. Currently, the widely used automated capillary electrophoresis-based short tandem repeat (STR) genotyping method for genetic screening in forensic practice is laborious, time-consuming, expensive, and technically challenging in some cases. Thus, new molecular-based strategies for conclusively identifying forensically relevant biological evidence are required. Here, we used high-resolution melting analysis (HRM) for Y-chromosome STR genotyping for forensic genetic screening. The reproducibility of the melting profile over dilution, sensitivity, discrimination power, and other factors was preliminarily studied in 10 Y-STR loci. The results showed that HRM-based approaches revealed more genotypes (compared to capillary electrophoresis), showed higher uniformity in replicate tests and diluted samples, and enabled successful detection of DNA at concentrations as low as 0.25 ng. For mixed samples, the melting curve profiles discriminated between mixed samples based on reference samples with high efficiency. The triplex Y-chromosome STR HRM assay was performed and provided a foundation for further studies such as a multiplex HRM assay. The HRM approach is a one-step application and the entire procedure can be completed within 2 h at a low cost. In conclusion, our findings demonstrate that the HRM-based Y-STR assay is a useful screening tool that can be used in forensic practice.

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“…Many of these primers were later adopted for use in commercial Y-STR kits such as PowerPlex Y and Yfiler. Core loci [19] and additional loci [21] have been examined in U.S. populations. Y-chromosomal duplications have also been examined [31].…”

Section: Y-chromosome Strsmentioning

confidence: 99%