High-Throughput SNP Scoring in a Disposable Microfabricated CD Device

Eckersten, Ann; Örlefors, Anna Edman; Ellström, Christel; Erickson, K.; Löfman, Esfir; Eriksson, Annica; Eriksson, Solveig; Jorsback, Anneli; Tooke, Nigel; Dérand, Helene; Ekstrand, Gunnar; Engström, Johan; Honerud, Ann-Kristin; Aksberg, Arvi; Hedsten, Helena; Rosengren, Lars; Stjernström, Mårten; Hultman, Thomas; Andersson, P.

doi:10.1007/978-94-017-2264-3_122

Cited by 10 publications

(5 citation statements)

References 2 publications

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“…Different microfluidic formats are currently being tested for pyrosequencing analysis. The pyrogram in Figure 6 demonstrates promising sequencing data with a relatively high signal-to-noise ratio that was obtained in a centrifugal force-driven compact disc microfluidic device (Eckersten et al 2000).…”

Section: Microfluidics Using Solid-phase Pyrosequencingmentioning

confidence: 83%

Pyrosequencing Sheds Light on DNA Sequencing

Ronaghi¹

2001

Genome Research

683

393

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DNA sequencing is one of the most important platforms for the study of biological systems today. Sequence determination is most commonly performed using dideoxy chain termination technology. Recently, pyrosequencing has emerged as a new sequencing methodology. This technique is a widely applicable, alternative technology for the detailed characterization of nucleic acids. Pyrosequencing has the potential advantages of accuracy, flexibility, parallel processing, and can be easily automated. Furthermore, the technique dispenses with the need for labeled primers, labeled nucleotides, and gel-electrophoresis. This article considers key features regarding different aspects of pyrosequencing technology, including the general principles, enzyme properties, sequencing modes, instrumentation, and potential applications.

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Section: Microfluidics Using Solid-phase Pyrosequencingmentioning

confidence: 83%

Pyrosequencing Sheds Light on DNA Sequencing

Ronaghi¹

2001

Genome Research

683

393

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“…With chip CE, working with much shorter separation channels is facilitated, allowing electric fields of 500−1000 V/cm and decreased analysis times as a result. − The integration of picoliter-volume injection elements into etched CE devices is a further unique feature of the chip format. Microfluidic devices operated using pressure-driven flow , and centrifugal pumping − have also been reported, expanding the range of possible analyses. The microfluidic approach should generally enable high throughput applications, − as well as a high level of automation for real-time testing at the point of care (POC). − …”

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confidence: 99%

An Integrated Fritless Column for On-Chip Capillary Electrochromatography with Conventional Stationary Phases

2002

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A new polymer device for use with conventional particulate stationary phases for on-chip, fritless, capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the particles of the stationary phase are retained and stabilized. The chips were easily fabricated in poly-(dimethylsiloxane) using deep-reactive-ion-etched silicon masters, and tested using a capillary electrophoretic separation of FITC-labeled amino acids. To perform CEC, the separation channel was packed using a vacuum with 3-µm, octadecylsilanized silica microspheres. The packing was stabilized in the column by a thermal treatment, and its stability and quality were evaluated using in-column indirect fluorescence detection. The effects of voltage on electro-osmotic flow and on efficiency were investigated, and the separation of two neutral compounds was achieved in less than 15 s.The use of microfabricated fluidic substrates has become increasingly well-established for liquid-phase analysis in recent years. This is particularly true for electrokinetically driven separations, where etched microchannels provide an easy route to reduced column diameters and, hence, increased efficiencies and decreased solvent/sample consumption. 1 As with narrow bore (<75 µm), fused-silica capillaries used in conventional capillary electrophoresis (CE) instrumentation, the high surface-to-volume ratios of these chip-based systems enable good dissipation of Joule heat. However, the shortest capillary that can be used in a benchtop instrument is generally no less than 30 cm long, which leads to typical applied electric field strengths on the order of a few hundred volts per cm. With chip CE, working with much shorter separation channels is facilitated, allowing electric fields of 500-1000 V/cm and decreased analysis times as a result. [2][3][4][5] The integration of picoliter-volume injection elements into etched CE devices is a further unique feature of the chip format. Microfluidic devices operated using pressure-driven flow 6,7 and centrifugal pumping [8][9][10][11] have also been reported, expanding the range of possible analyses. The microfluidic approach should generally enable high throughput applications, 12-14 as well as a high level of automation for real-time testing at the point of care (POC). [15][16][17] The many advantages of microfabricated devices for ultrasmallscale analysis have been demonstrated with a wide variety of examples, including sample preconcentration, 18,19 cell handling, 20,21

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“…In one particular system, signal amplification is achieved via tagging of the target DNA with gold nanoparticles and detection is based on measuring the amount of subsequent electrocatalytic deposition of silver metal onto the nanoparticles [ 165 ]. Other integration formats include the innovative compact disk device, also known as LabCD [ 166 ], a commercial product which utilizes centrifugal forces for pumping of fluids through reservoirs, valves, mixing chambers, and heating chambers. The control of flow rates through the device is tuned via different disk spin speeds, and is capable of achieving sample preparation, DNA purification, and PCR amplification.…”

Section: Integrated Pathogen Detection Systemsmentioning

confidence: 99%

Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems

Lui

Cady

Batt

2009

Sensors

105

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The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform.

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High-Throughput SNP Scoring in a Disposable Microfabricated CD Device

Cited by 10 publications

References 2 publications

Pyrosequencing Sheds Light on DNA Sequencing

Pyrosequencing Sheds Light on DNA Sequencing

An Integrated Fritless Column for On-Chip Capillary Electrochromatography with Conventional Stationary Phases

Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems

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