High-throughput sequencing of Fasciola spp. shows co-infection and intermediate forms in Balochistan, but only Fasciola gigantica in the Punjab province of Pakistan

Rehman, Zia Ur; Martin, K; Zahid, Osama; Ali, Qasim; Rashid, Imran; Hafeez, Mian Abdul; Ahmad, Nisar; Ashraf, Kamran; Betson, Martha; Sargison, Neil; Chaudhry, Umer

doi:10.1016/j.meegid.2021.105012

Cited by 8 publications

(12 citation statements)

References 33 publications

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“…Data was collected from a randomized population, which included both literate and illiterate people from different ethnic groups (i.e., Punjabi, Pakhtoon, Saraiki, and Urdu speaking). The 27.2% participants were Punjabis and the previous studies have reported the 15.2% and 13.6% prevalence of schistosomiasis during the years 2011 and 2013, respectively (9, 25) whereas a latest study was conducted in 2021 and 18 ruminants (cattle, buffalo, sheep, and goat) were screened and all were positive for fascioliasis in Punjab (26). 21.5% participants were from capital territory Islamabad and the prevalence of fascioliasis in Islamabad is documented 1.5% according to a latest analysis (27).…”

Section: Relationship Between the Prevalence Of Schistosomiasis And F...mentioning

confidence: 85%

Knowledge, attitudes and practices related to neglected tropical diseases (schistosomiasis and fascioliasis) of public health importance: A cross-sectional study

et al. 2023

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BackgroundSnails play an important role as an intermediate host in various parasitic diseases, which are referred to as snail-borne parasitic diseases (SBPDs). The prevalence of the SBPDs, schistosomiasis and fascioliasis is low in Pakistan compared to other countries. The present study investigated knowledge, attitudes, and practices related to these two SPBDs and risk factors associated with them among the humans living in close contact with livestock and pets from three regions of Pakistan: Punjab, Islamabad and Azad Jammu and Kashmir (AJK).MethodsA cross-sectional survey was conducted using a structured questionnaire to assess participants' knowledge, attitude and practices related to schistosomiasis and fascioliasis during 2021–2022.ResultsThe majority of the 507 participants who were interviewed had good knowledge of schistosomiasis and fascioliasis: 43% were aware of schistosomiasis and 57% were aware of fascioliasis, but only 25% knew about snails as an intermediate host. Most respondents had a positive attitude toward treatment, prevention and control of the diseases but they did not have any healthcare facilities.ConclusionThis study highlights the importance of the public's awareness for the need to control SBPDs. It also draws attention to the need for health education for risk reduction and the prevention of SBPDs in endemic areas.

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Section: Relationship Between the Prevalence Of Schistosomiasis And F...mentioning

confidence: 85%

Knowledge, attitudes and practices related to neglected tropical diseases (schistosomiasis and fascioliasis) of public health importance: A cross-sectional study

et al. 2023

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“…Moreover, specimens of Fasciola sp are reported as 'intermediate forms' (Le et al, 2008;Rehman et al, 2021;Sumruayphol et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Sharbatkhori

Nasibi

Mohammadi

et al. 2023

Veterinary Medicine & Sci

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Background: Fascioliasis, caused by the liver flukes Fasciola hepatica and Fasciola gigantica, is a global zoonotic helminthic disease. The livestock and human are the final hosts of the parasites. Northern Iran is an important endemic region for fascioliasis. Few studies have been conducted on the characterization of Fasciola isolates from eastern regions of the Caspian littoral of the country. Objective:The aim of the present study was to identify F. hepatica, F. gigantica and intermediate/hybrid forms of Fasciola isolates from livestock in Golestan province, northern Iran, using morphometric and molecular tools.Methods: Livestock livers naturally infected with Fasciola spp. were collected from Golestan slaughterhouse during 2019-2020. The worms were morphometrically studied using a calibrated stereomicroscope. Genomic DNA was extracted from all samples, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on internal transcribed spacer (ITS1) region using Rsa1 restriction enzyme. All the isolates were then analysed by multiplex PCR on Pepck region.Results: A total of 110 Fasciola isolates were collected from the infected livers, including 94 sheep, 12 cattle and 4 goats. Morphometric analysis of 61 adult Fasciola isolates indicated that, 44 and 17 isolates belonged to F. hepatica and F. gigantica, respectively.Eighty-one and 29 isolates belonged to F. hepatica and F. gigantica using ITS1-RFLP, respectively. However, Pepck Multiplex PCR indicated 72 F. hepatica, 26 F. gigantica and 12 intermediate/hybrid forms. All 12 hybrid isolates were found in sheep host. Two isolates were identified as F. gigantica using morphometry and F. hepatica using both molecular methods. Conclusion:The present study confirmed the existence of both F. hepatica and F. gigantica species and reported the first molecular evidence of hybrid Fasciola isolates in ruminants of Golestan province.

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“…Moreover, although there are various methods used to the diagnosis of fasciolosis, each has its limitations and disadvantages. For example, the presence of cross-reactions in serological diagnosis, ineffectiveness of parasitological methods in the diagnosis of acute and chronic stages of fasciolosis are reported ( 7 , 8 – 12 , 13 ), whereas molecular methods are more specific (Real-time PCR or semi-nested PCR) than other methods ( 22 – 25 ). Similarly, comparisons between diagnostic methods (molecular, serological and microscopic) have always been proposed in parasitic diseases to reach a preferred method.…”

Section: Discussionmentioning

confidence: 99%

“…Preparation and protein concentration of the ES antigen from adult F. gigantica was performed as reported already ( 7 ). All serum samples were examined by indirect ELISA method based on a previous study ( 13 ).…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

Alizadeh¹,

Aryaeipour²,

Mohebali³

et al. 2022

ijph

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Background: We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis. Methods: Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis. Individuals were classified based on diagnostic of fascioliasis and habitat in endemic areas. Finally, all serum samples were tested by indirect ELISA (using secretory excretory antigen) and semi-nested PCR (using ITS1 gene). The study was conducted in the School of Publish Health, Tehran University of Medical Sciences, Iran in 2021. Results: Significant differences were found between agreement and similarity of patients' results of indirect ELISA and semi-nested PCR 94.46% and 98.4% respectively (Cohen's kappa ≥0.6; P-value≤0.05). No cross-reactions were observed with other parasitic diseases (toxocariasis, hydatidosis, strongyloidiasis, toxoplasmosis, cutaneous leishmaniasis, taeniasis and trichinosis). 69.84% of samples were positive by both techniques. In addition, the percentage of agreement and similarity between the results of the two techniques based on habitat in endemic areas was 88.9-100% and 97.7-100%, respectively (Cohen's kappa ≥0.6; P-value≤0.05). Conclusion: Semi-nested PCR could be a suitable method for following up on patients' treatment and a confirmatory method for ELISA as for diagnosis of human fascioliasis.

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High-throughput sequencing of Fasciola spp. shows co-infection and intermediate forms in Balochistan, but only Fasciola gigantica in the Punjab province of Pakistan

Cited by 8 publications

References 33 publications

Knowledge, attitudes and practices related to neglected tropical diseases (schistosomiasis and fascioliasis) of public health importance: A cross-sectional study

Knowledge, attitudes and practices related to neglected tropical diseases (schistosomiasis and fascioliasis) of public health importance: A cross-sectional study

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis

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