High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition

Xu, Lei; Wu, Zhifang; Wang, Yuan; Wang, Sa; Shu, Chang; Duan, Zhuhui; Deng, Shengjue

doi:10.1631/jzus.b2000554

Cited by 4 publications

(3 citation statements)

References 47 publications

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“…Hence, how Veillonella plays a role in the occurrence and development of CA and ECC in children needs needs more evidences and further research. Furthermore, we also found that Gemella were significantly enriched in the LA group, while Xu et al., 2021b also considered it to be one of the core microflora in the oral saliva of caries-free preschool children, suggesting that Gemella may be a beneficial microflora to maintain oral health of children.…”

Section: Discussionmentioning

confidence: 53%

Correlation between caries activity and salivary microbiota in preschool children

Lin

Wang²,

et al. 2023

Front. Cell. Infect. Microbiol.

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Early childhood caries (ECC) is the most common chronic infectious oral disease in preschool children worldwide. It is closely related to the caries activity (CA) of children. However, the distribution characteristics of oral saliva microbiomes in children with different CA are largely underexplored. The aim of this study was to investigate the microbial community in saliva of preschool children with different CA and caries status, and to analyze the difference of microbial community in saliva of children with different CA and its correlation with ECC. Subjects were divided into 3 groups based on the Cariostat caries activity test: Group H, high CA (n=30); Group M, medium CA (n = 30); Group L, low CA (n=30). Questionnaire survey was used to explore the related influencing factors of CA. According to the caries status (on the basis of decayed mising filled teeth), these subjects were divided into caries-free group (dmft=0, n=19), caries-low group (0 < dmft ≤ 4, n=27) and caries-high group (dmft > 4, n=44). Microbial profiles of oral saliva were analyzed using 16S rRNA gene sequencing. There were significant differences in the microbial structure (P < 0.05). Scardovia and Selenomonas were the biomarkers of both H group and high caries group. The genus Abiotrophia and Lautropia were the biomarkers of both the L group and the low caries group, while the Lactobacillus and Arthrospira spp. were significantly enriched in the M group. The area under the ROC curve of the combined application of dmft score, age, frequency of sugary beverage intake, and the genus Scardovia, Selenomonas, and Campylobacter in screening children with high CA was 0.842. Moreover, function prediction using the MetaCyc database showed that there were significant differences in 11 metabolic pathways of salivary microbiota among different CA groups. Certain bacteria genera in saliva such as Scardovia and Selenomonas may be helpful in screening children with high CA.

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Section: Discussionmentioning

confidence: 53%

Correlation between caries activity and salivary microbiota in preschool children

Lin

Wang²,

et al. 2023

Front. Cell. Infect. Microbiol.

Self Cite

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“…et al [ 7 ] 2022 3 1 3 7 Xu H. et al [ 17 ] 2022 2 1 2 5 Li F. et al [ 20 ] 2021 3 2 2 7 Lee E. et al [ 19 ] 2021 3 2 3 8 Arweiler N.B. et al [ 18 ] 2021 3 1 2 6 Xu L. et al [ 23 ] 2021 2 2 3 7 Jo R. et al [ 21 ] 2021 2 1 3 6 Qudeimat M.A. et al [ 22 ] 2021 <...…”

Section: Table A1mentioning

confidence: 99%

“…et al, 2021 [ 22 ] Caries-active and caries-free children have overlapping diversity indexes. Xu L. et al, 2021 [ 23 ] Analysis of microbial diversity and community structure revealed a similar pattern between male and female healthy subjects. Kahharova D. et al, 2020 [ 24 ] The oral ecosystem of caries-free toddlers is highly heterogeneous and dynamic, with substantial changes in microbial composition over time and only few taxa persisting across the 3 y of the study.…”

Section: Table A1mentioning

confidence: 99%

Exploring Oral Microbiome in Healthy Infants and Children: A Systematic Review

D’Agostino

Ferrara

Valentini

et al. 2022

IJERPH

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Recent advances in the development of next-generation sequencing (NGS) technologies, such as the 16S rRNA gene sequencing, have enabled significant progress in characterizing the architecture of the oral microbiome. Understanding the taxonomic and functional components of the oral microbiome, especially during early childhood development, is becoming critical for identifying the interactions and adaptations of bacterial communities to dynamic conditions that may lead to the dysfunction of the host environment, thereby contributing to the onset and/or progression of a wide range of pathological conditions. We aimed to provide a comprehensive overview of the most recent evidence from studies of the oral microbiome of infants and young children, focusing on the development of oral microbiome in the window of birth to 18 years, focusing on infants. A systematic literature search was conducted in PubMed, Scopus, WOS, and the WHO clinical trial website for relevant articles published between 2006 to 2022 to identify studies that examined genome-wide transcriptome of the oral microbiome in birth, early childhood, and adolescence performed via 16s rRNA sequence analysis. In addition, the references of selected articles were screened for other relevant studies. This systematic review was performed in accordance PRISMA guidelines. Data extraction and quality assessment were independently conducted by two authors, and a third author resolved discrepancies. Overall, 34 studies were included in this systematic review. Due to a considerable heterogeneity in study population, design, and outcome measures, a formal meta-analysis was not carried out. The current evidence indicates that a core microbiome is present in newborns, and it is stable in species number. Disparity about delivery mode influence are found. Further investigations are needed.

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Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis

Huang

Liu

Bai

et al. 2022

J. Zhejiang Univ. Sci. B

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High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition

Cited by 4 publications

References 47 publications

Correlation between caries activity and salivary microbiota in preschool children

Correlation between caries activity and salivary microbiota in preschool children

Exploring Oral Microbiome in Healthy Infants and Children: A Systematic Review

Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis

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