High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment

Ankam, Soneela; Teo, Benjamin Kim Kiat; Kukumberg, Marek; Yim, Evelyn K.F.

doi:10.4161/org.25425

Cited by 34 publications

(22 citation statements)

References 77 publications

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“…Microfluidics for analysing stem cell niches devices that could also be used for the analysis and screening of chemical and mechanical factors affecting stem cell niches (Ankam et al, 2013;Gupta et al, 2010). Microfluidic devices are currently being developed, with the goal of mimicking all the components that regulate stem cell niche physiology.…”

Section: Microfluidics For Stem Cell Niches Modellingmentioning

confidence: 99%

“…Therefore, microfluidic platforms allow the miniaturisation and mimicking of the complex microenvironment of stem cell niches, thus providing a better control of variations within the niches in a high throughput and scalable manner (Titmarsh et al, 2014). The newest technological developments led to the improvement of these microfluidic 220 www.ecmjournal.org P Pagella et alMicrofluidics for analysing stem cell niches devices that could also be used for the analysis and screening of chemical and mechanical factors affecting stem cell niches (Ankam et al, 2013;Gupta et al, 2010). Microfluidic devices are currently being developed, with the goal of mimicking all the components that regulate stem cell niche physiology.…”

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confidence: 99%

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Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Pagella

Neto

Lamghari

et al. 2015

eCM

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Stem cell-based mediated therapies represent very promising approaches for tissue regeneration and are already applied with success in clinics. These therapeutic approaches consist of the in vitro manipulation of stem cells and their consequent administration to patients as living and dynamic biological agents. Nevertheless, the deregulation of stem cells function might result in the generation of pathologies such as tumours or accelerated senescence. Moreover, different stem cells sources are needed for regeneration of specific tissues. It is thus fundamental to understand the mechanisms regulating the physiology of stem cells. Microfluidic technology can be used to mimic in vivo scenarios and allow the study of stem cell physiology at both single cell and whole stem cell niche levels. This review focuses on the potential sources of stem and progenitor cells for orofacial regeneration and the use of microfluidic technologies for the study of stem cells behaviour and stem cell niches, in the light of regenerative medicine. Keywords:Microfluidics, stem cells, stem cell niches, orofacial regeneration, tooth, innervation, trigeminal ganglia, regenerative medicine. IntroductionThe development of organs and tissues that belong to the orofacial complex proceeds through a series of inductive interactions between cells originated from the epithelium, mesoderm and cranial neural crest-derived mesenchyme (Mao et al., 2012;Mitsiadis and Graf, 2009;Mitsiadis and Papagerakis, 2011). Orofacial organs are highly diverse and exert fundamental and specific functions such as breathing, chewing, speech, smell, and sight (Mao et al., 2012). The physiological functions of these organs are compromised by traumatic injuries, congenital and infectious diseases, and cancer (Mao et al., 2012;Scheller et al., 2009). Furthermore, these pathologies are often accompanied by intensive pain and aesthetic deformities. Therefore, the treatment of compromised pathological orofacial tissues and organs should guarantee restoration of both functionality and aesthetics, which constitutes an enormous clinical challenge. Moreover, organ structure, function, aesthetics, and pain should be managed simultaneously during the regenerative care, a situation that is more complex than in other compartments of the body (Scheller et al., 2009).Biological regeneration is proving an increasingly attractive alternative and complement to traditional surgical techniques for prosthetic replacement of tissues and organs. Cell-based therapeutic approaches are already applied with success in clinics and consist of in vitro manipulation of stem cells and their consequent administration to patients as living and dynamic biological agents. Stem cells are characterised by their potential to self-replicate and their capacity to differentiate into a vast variety of cell types that form the diverse tissues. Therefore, stem cells guarantee tissue repair and regeneration throughout life. During the last decades, a plethora of adult stem cell populations have been isolate...

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Section: Microfluidics For Stem Cell Niches Modellingmentioning

confidence: 99%

mentioning

confidence: 99%

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Pagella

Neto

Lamghari

et al. 2015

eCM

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“…Recent developments in biomaterial science allow direct investigation of the processes that control neuronal contact sensing by using specific nanotextured substrates . Nano/microstructured surfaces are capable of tuning the morpho‐functional properties of neurons, and can be a powerful tool to study cell dynamical responses to physical stimuli in vitro . In particular, nano/microgratings (NGs, anisotropic topographies composed of alternating lines of grooves and ridges with submicrometer lateral dimensions) could effectively apply directional topographical stimuli via pure contact interaction, and tune neuronal differentiation, polarity, and neurite orientation .…”

Section: Introductionmentioning

confidence: 99%

Impaired Neurite Contact Guidance in Ubiquitin Ligase E3a (Ube3a)‐Deficient Hippocampal Neurons on Nanostructured Substrates

Tonazzini

Meucci

Woerden

et al. 2016

Adv Healthcare Materials

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Recent discoveries indicate that during neuronal development the signaling processes that regulate extracellular sensing (e.g., adhesion, cytoskeletal dynamics) are important targets for ubiquitination-dependent regulation, in particular through E3 ubiquitin ligases. Among these, Ubiquitin E3a ligase (UBE3A) has a key role in brain functioning, but its function and how its deficiency results in the neurodevelopmental disorder Angelman syndrome is still unclear. Here, the role of UBE3A is investigated in neurite contact guidance during neuronal development, in vitro. The microtopography sensing of wild-type and Ube3a-deficient hippocampal neurons is studied by exploiting gratings with different topographical characteristics, with the aim to compare their capabilities to read and follow physical directional stimuli. It is shown that neuronal contact guidance is defective in Ube3a-deficient neurons, and this behavior is linked to an impaired activation of the focal adhesion signaling pathway. Taken together, the results suggest that the neuronal contact sensing machinery might be affected in Angelman syndrome.

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“…Additive patterning of biomaterials with high resolution in three dimensions may enable controlled cellular environments that are central to tissue engineering [ 248 ] and biology. [249][250][251] Direct and high-resolution printing of individual cells and components of an extracellular matrix may play a key role in further developments in this area. [ 252 ] A relatively unexplored but growing area of opportunity is in the fabrication of photonic and plasmonic devices, where patterning of nanomaterials at high resolution is important.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms, Capabilities, and Applications of High‐Resolution Electrohydrodynamic Jet Printing

Önses

Sutanto

Ferreira

et al. 2015

Small

459

397

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This review gives an overview of techniques used for high-resolution jet printing that rely on electrohydrodynamically induced flows. Such methods enable the direct, additive patterning of materials with a resolution that can extend below 100 nm to provide unique opportunities not only in scientific studies but also in a range of applications that includes printed electronics, tissue engineering, and photonic and plasmonic devices. Following a brief historical perspective, this review presents descriptions of the underlying processes involved in the formation of liquid cones and jets to establish critical factors in the printing process. Different printing systems that share similar principles are then described, along with key advances that have been made in the last decade. Capabilities in terms of printable materials and levels of resolution are reviewed, with a strong emphasis on areas of potential application.

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High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment

Cited by 34 publications

References 77 publications

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Impaired Neurite Contact Guidance in Ubiquitin Ligase E3a (Ube3a)‐Deficient Hippocampal Neurons on Nanostructured Substrates

Mechanisms, Capabilities, and Applications of High‐Resolution Electrohydrodynamic Jet Printing

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