High‐Throughput Screening and Hierarchical Topography‐Mediated Neural Differentiation of Mesenchymal Stem Cells

Yang, Liangliang; Jurczak, Klaudia Malgorzata; Ge, Lu; Rijn, Patrick van

doi:10.1002/adhm.202000117

Cited by 41 publications

(38 citation statements)

References 57 publications

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“…At the current stage, although the role of magnetic properties and anisotropicity in the induction of ADSC osteogenic differentiation should be fully elucidated before considering animal-based validation experiments, it is still suitable in vitro platform for mechanistic studies and therapeutic candidate testing. In addition, we believe that combination with cell-derived ECM will improve the cell-composite interaction and further promote the osteogenesis of stem cells [32][33][34] .…”

Section: Resultsmentioning

confidence: 99%

Dual anisotropicity comprising 3D printed structures and magnetic nanoparticle assemblies: towards the promotion of mesenchymal stem cell osteogenic differentiation

Tang

et al. 2021

NPG Asia Mater

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Leveraging physical factors in cellular microenvironments to promote adipose tissue-derived stem cell (ADSC) osteogenic differentiation has emerged as a new strategy in the development of scaffolds for bone tissue engineering. Anisotropicity is one of those factors of interest; however, the utilization of anisotropicity to promote ADSC osteogenic differentiation is still not efficient. In this study, we designed a substrate with a dual anisotropic structure fabricated via a combination of 3D printing and magnetic field-induced magnetic nanoparticle assembly techniques. These dual anisotropic structures have a scale hierarchy, and the scale of the magnetic nanoparticle assemblies matches that of a single ADSC. This is in contrast to conventional anisotropic osteogenic induction scaffolds that have anisotropic structures at only one scale and at an order of magnitude different from single ADSCs. ADSCs cultured on substrates with such structures have significantly higher osteogenic marker expression, e.g., ALP, at both the protein and mRNA levels, and more calcium nodule formation was also found, suggesting a stronger tendency toward osteogenic differentiation of ADSCs. RNA-seq data revealed that alterations in kinase signaling pathway transduction, cell adhesion, and cytoskeletal reconstruction may account for the elevated osteogenic induction capacity. These data support our hypothesis that such a structure could maximize the anisotropicity that ADSCs can sense and therefore promote ADSC osteogenic differentiation.

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Section: Resultsmentioning

confidence: 99%

Dual anisotropicity comprising 3D printed structures and magnetic nanoparticle assemblies: towards the promotion of mesenchymal stem cell osteogenic differentiation

Tang

et al. 2021

NPG Asia Mater

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“… 12 Wave-like topography gradients with a height between 541 and 3073 nm and wavelengths increasing from 4 to 30 μm were used to study the fate commitment of MSCs toward neuron lineage. 173 They found that the substrate with wavelength 26 μm/height 2.9 μm was optimum for neuron differentiation ( Figure 14 A). These results demonstrate that an anisotropic gradient platform can serve as an effective system to obtain the optimum parameter for specific cellular behaviors, which could improve regenerative medicine of stem cell therapies.…”

Section: Gradient-based Hts Approaches For Biomaterials Discovery and Materiobiologymentioning

confidence: 99%

“…With this method, the van Rijn group prepared PDMS-based wrinkled topography gradients with varied wavelengths (W) and amplitudes (A). 34 , 100 , 170 − 173 Table 2 presents an overview of varied fabrication techniques for the creation of topography gradients.…”

Section: Gradient-based Hts Approaches For Biomaterials Discovery and Materiobiologymentioning

confidence: 99%

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High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

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The complex interaction of cells with biomaterials (i.e., materiobiology) plays an increasingly pivotal role in the development of novel implants, biomedical devices, and tissue engineering scaffolds to treat diseases, aid in the restoration of bodily functions, construct healthy tissues, or regenerate diseased ones. However, the conventional approaches are incapable of screening the huge amount of potential material parameter combinations to identify the optimal cell responses and involve a combination of serendipity and many series of trial-and-error experiments. For advanced tissue engineering and regenerative medicine, highly efficient and complex bioanalysis platforms are expected to explore the complex interaction of cells with biomaterials using combinatorial approaches that offer desired complex microenvironments during healing, development, and homeostasis. In this review, we first introduce materiobiology and its high-throughput screening (HTS). Then we present an in-depth of the recent progress of 2D/3D HTS platforms (i.e., gradient and microarray) in the principle, preparation, screening for materiobiology, and combination with other advanced technologies. The Compendium for Biomaterial Transcriptomics and high content imaging, computational simulations, and their translation toward commercial and clinical uses are highlighted. In the final section, current challenges and future perspectives are discussed. High-throughput experimentation within the field of materiobiology enables the elucidation of the relationships between biomaterial properties and biological behavior and thereby serves as a potential tool for accelerating the development of high-performance biomaterials.

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“…Nowadays, hydrophilic and hydrophobic materials can be applied in a significant manner, in many sectors, such as biomedical, antifogging techniques for hydrophilic ones; whereas hydrophobic materials can be used to remove petroleum or oil from aqueous solutions, even applied to ceramics and plastics (Drelich et al, 2011;Su et al, 2016;Wang et al, 2017). The goal is the realization of innovative and simplified biomaterials, capable to provide a better understanding of neuronal mechanisms and able to simulate the outer microenvironment of neuronal cells, and at the same time, suitable even for in vivo applications (Yang et al, 2020). For these reasons, it is essential to manipulate the mechanical biomaterial properties together with the biological signals to improve proliferation, migration and differentiation.…”

Section: Biomaterials Applications For Neural Tissue Engineeringmentioning

confidence: 99%

A State-of-the-Art of Functional Scaffolds for 3D Nervous Tissue Regeneration

Tupone

Castelli

Catanesi

et al. 2021

Front. Bioeng. Biotechnol.

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Exploring and developing multifunctional intelligent biomaterials is crucial to improve next-generation therapies in tissue engineering and regenerative medicine. Recent findings show how distinct characteristics of in situ microenvironment can be mimicked by using different biomaterials. In vivo tissue architecture is characterized by the interconnection between cells and specific components of the extracellular matrix (ECM). Last evidence shows the importance of the structure and composition of the ECM in the development of cellular and molecular techniques, to achieve the best biodegradable and bioactive biomaterial compatible to human physiology. Such biomaterials provide specialized bioactive signals to regulate the surrounding biological habitat, through the progression of wound healing and biomaterial integration. The connection between stem cells and biomaterials stimulate the occurrence of specific modifications in terms of cell properties and fate, influencing then processes such as self-renewal, cell adhesion and differentiation. Recent studies in the field of tissue engineering and regenerative medicine have shown to deal with a broad area of applications, offering the most efficient and suitable strategies to neural repair and regeneration, drawing attention towards the potential use of biomaterials as 3D tools for in vitro neurodevelopment of tissue models, both in physiological and pathological conditions. In this direction, there are several tools supporting cell regeneration, which associate cytokines and other soluble factors delivery through the scaffold, and different approaches considering the features of the biomaterials, for an increased functionalization of the scaffold and for a better promotion of neural proliferation and cells-ECM interplay. In fact, 3D scaffolds need to ensure a progressive and regular delivery of cytokines, growth factors, or biomolecules, and moreover they should serve as a guide and support for injured tissues. It is also possible to create scaffolds with different layers, each one possessing different physical and biochemical aspects, able to provide at the same time organization, support and maintenance of the specific cell phenotype and diversified ECM morphogenesis. Our review summarizes the most recent advancements in functional materials, which are crucial to achieve the best performance and at the same time, to overcome the current limitations in tissue engineering and nervous tissue regeneration.

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High‐Throughput Screening and Hierarchical Topography‐Mediated Neural Differentiation of Mesenchymal Stem Cells

Cited by 41 publications

References 57 publications

Dual anisotropicity comprising 3D printed structures and magnetic nanoparticle assemblies: towards the promotion of mesenchymal stem cell osteogenic differentiation

Dual anisotropicity comprising 3D printed structures and magnetic nanoparticle assemblies: towards the promotion of mesenchymal stem cell osteogenic differentiation

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

A State-of-the-Art of Functional Scaffolds for 3D Nervous Tissue Regeneration

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