High-throughput screen for high performance microalgae strain selection and integrated media design

Wolf, Juliane; Ross, Ian L.; Radzun, Khairul Adzfa; Jakob, Gisela; Stephens, Evan; Hankamer, Ben

doi:10.1016/j.algal.2015.07.005

Cited by 31 publications

(26 citation statements)

References 35 publications

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“…braunii is thus a complex biological model, with high variability of physiological behaviors [9] that needs to be taken into account for the optimization of its cultivation [10]. Growing conditions could be optimized for B. braunii, as already done for other microalgae models, using medium-and high-throughput cultivation screening approach (MTCS) through response surface methodology [11][12][13]. Nevertheless, it still makes strains characterization laborious, particularly for the study of their biofuel potential.…”

Section: Doubling Time Maxmentioning

confidence: 99%

Development of a screening procedure for the characterization of Botryococcus braunii strains for biofuel application

Moutel

Gonçalves

Grand

et al. 2016

Process Biochemistry

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00001 ăWOS:000388058100012International audienceAn integrated screening approach was developed to assess the potential of the Botryococcus genus for the production of lipids for biofuel application (hydrocarbons and triacylglycerols). The strategy developed in this study permitted to rigorously measure mandatory parameters for the determination of strain performance - i.e. growth rate and oil content. For that purpose, mini-photobioreactors run in parallel were used together with an exhaustive lipid class analysis. The quantitative productivity measurements indicated that the ten screened strains presented very different patterns in the conditions of PBR culture, firstly run in batch, and secondly in continuous mode. Indeed, with the applied setup, only four strains presented biomass productivities close to that previously measured for the reference strain 807/1 (between 74 and 307 mg L-1 day(-1) of biomass for the tested strains). HC productivity obtained under continuous light (similar to 14 mg L-1 day(-1)) was close to that measured for the strain 807/1 (similar to 20 mg L-1 day(-1)), confirming B. braunii tested strains potential for biofuel application. The analysis of the lipid chemodiversity revealed moreover that this genus should be further investigated for its polar lipids, as it suggests potential pharmaceutical applications such as production of antitumorals or immunosuppressors. (C) 2016 Elsevier Ltd. All rights reserved

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Section: Doubling Time Maxmentioning

confidence: 99%

Development of a screening procedure for the characterization of Botryococcus braunii strains for biofuel application

Moutel

Gonçalves

Grand

et al. 2016

Process Biochemistry

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“…However, a majority of the microalgae species used ammonium as the source of nitrogen because it is in a reduced form that is ready to be taken up by the algae cells. In contrast, nitrate assimilation requires energy for the reduction process (NADH usage and active transport via NO 3-) [19], [27]. The concentration of silicon in M2 (0.236 mM) is lower than in M1 (0.472 mM).…”

Section: A Improvement Of N Oleoabundans Cultivation Mediummentioning

confidence: 97%

“…Other nutrient elements and chemicals were supplied at a constant concentration as tabulated in Table 1. Formulations of the nutrient media and experimental design were conducted according to Radzun et al [20] and Wolf, J. et al [19] respectively. In this work, Box-Behnken statistical design was used to generate 180 nutrient media formulations matrix, and TP control medium replicates.…”

Section: B Automated Microalgal Nutrient Screening Systemmentioning

confidence: 99%

“…Via Automated Nutrient Screening System The automated nutrient screening [20] was designed to define strain specific media with non-limiting nutrients which can also minimise costs and to increase the productivity of the microalgae. The nutrient screening was operated using a screen matrix comprising of 183 microwells with different concentration of nutrients in each well (180 media solution and 3 replicates of TP control medium) [19]. The cultivation of the microalgae in the automated nutrient screening system was conducted under photoautotrophic condition.…”

Section: A Improvement Of N Oleoabundans Cultivation Mediummentioning

confidence: 99%

“…Therefore, the optimisation of nutrient in media is necessary to identify the best growth condition for microalgae. The high-throughput nutrient optimisation screen was used in this study where a large multi-dimensional statistical space matrix was used to identify improved production in terms of Ca, Mg, B, Fe, Cu, Mn, Zn, Se, V and Si [19]. The objective of this study was to optimisation the nutrients that required by Neochloris oleoabundans in order to obtain high biomass density.…”

Section: Introductionmentioning

confidence: 99%

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Development of Improved Formulation for Neochloris oleoabundans Via an Automated Microalgal Nutrient Screening System

Ibrahim

Radzun

Ismail

2018

International Journal on Advanced Science, Engineering and Information Technology

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Microalgal biomass has been regarded as one of the most promising commodities for biofuels production, fertilisers, animal food and high-value products. The capacity to generate high microalgal biomass is hence crucial to increase the number of the product that can be derived microalgal biomass. Recently, an advanced high-throughput nutrient screen was used to optimise the nutrient formulation to maximise the growth of Neochloris oleoabundans under photoautotrophic condition. The screen was designed to optimise 10 nutrients via the Box-Behnken experimental design that established a screen comprising 180 media formulations matrix for microalgae cultivation (150 µL microplate-based miniature-scale cultivation system). Improved cultivation media were formulated for N. oleoabundans via the nutrient screening system. Medium formulation no. 51 and 76 (from the 180 media formulation) yielded the highest end-point OD 560 of 1.319 ±0. 009 and 1.345 ±0. 023 respectively and both formulations were redesignated as medium M1 and M2 respectively for subsequent experiments. Reproducibility assessment of both media (1 L larger scale experiments) was in concordance with miniature scale data. Nutrient main effect statistical analysis suggested that modulation of Ca, Mg, Fe, Mn and Zn concentrations may further improve the N. oleoabundans growth performance.

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Sustainable Production of Pigments from Cyanobacteria

Deepika

Wolf

Roles

et al. 2022

Advances in Biochemical Engineering/Biotechnology

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High-throughput screen for high performance microalgae strain selection and integrated media design

Cited by 31 publications

References 35 publications

Development of a screening procedure for the characterization of Botryococcus braunii strains for biofuel application

Development of a screening procedure for the characterization of Botryococcus braunii strains for biofuel application

Development of Improved Formulation for Neochloris oleoabundans Via an Automated Microalgal Nutrient Screening System

Sustainable Production of Pigments from Cyanobacteria

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