Development of a screening procedure for the characterization of Botryococcus braunii strains for biofuel application

Abstract

00001 ăWOS:000388058100012International audienceAn integrated screening approach was developed to assess the potential of the Botryococcus genus for the production of lipids for biofuel application (hydrocarbons and triacylglycerols). The strategy developed in this study permitted to rigorously measure mandatory parameters for the determination of strain performance - i.e. growth rate and oil content. For that purpose, mini-photobioreactors run in parallel were used together with an exhaustive lipid class analysis. The quantitative productivity measurements indicated that the ten screened strains presented very different patterns in the conditions of PBR culture, firstly run in batch, and secondly in continuous mode. Indeed, with the applied setup, only four strains presented biomass productivities close to that previously measured for the reference strain 807/1 (between 74 and 307 mg L-1 day(-1) of biomass for the tested strains). HC productivity obtained under continuous light (similar to 14 mg L-1 day(-1)) was close to that measured for the strain 807/1 (similar to 20 mg L-1 day(-1)), confirming B. braunii tested strains potential for biofuel application. The analysis of the lipid chemodiversity revealed moreover that this genus should be further investigated for its polar lipids, as it suggests potential pharmaceutical applications such as production of antitumorals or immunosuppressors. (C) 2016 Elsevier Ltd. All rights reserved

Allelochemicals from Alexandrium minutum induce rapid inhibition of metabolism and modify the membranes from Chaetoceros muelleri

“…Lipid class composition analyses were performed by high-performance thin layer chromatography (HPTLC) using a CAMAG auto-sampler to spot the sample on HPTLC glass plates pre-coated with silica gel (Merck & Co., Ltd., Darmstadt, Germany). Neutral and polar lipid classes were analyzed according to (Moutel et al, 2016). For polar and neutral lipid fatty acid (FA) analysis, an aliquot of the chloroform: methanol (2:1, v:v) extract was dried under N 2(g) and then resuspended in chloroform:methanol (98:2, v:v) prior to neutral and polar lipid separation.…”

Photobioreactor cultivation strategies for microalgae and cyanobacteria

“…Gokulan et al determined that the microalgae Botryococcus braunii was capable of reducing nitrogen content in greywater from 14.21 ppm to 0.31 ppm and phosphorous from 9.64 ppm to 2.16 ppm . B. braunii is a promising microalgae strain for biofuel production due to its high productivity of hydrocarbon oils . In another study, two microalgal isolates reduced the chemical oxygen demand (COD) by 70% and NH 3 ‐N by 100% in unsterile drain wastewater containing greywater generated from ∼400 households in India.…”

Peer Review #1 of "Associated bacteria of Botryococcus braunii (Chlorophyta) (v0.1)"

“…B. braunii comprises of a variety of strains from diverse parts of the world. The strains can differ in the hydrocarbon and exopolysaccharide content (Allard and Casadevall, 1875;Dayananda et al, 2007;Eroglu et al, 2011;Gouveia et al, 2017;Metzger et al, 1988;Moutel et al, 1855;Volova et al, 1998;Wolf, 1983).…”