High-throughput quantitation method for amodiaquine and desethylamodiaquine in plasma using supported liquid extraction technology

Abstract

Highlights Supported liquid extraction is easy to use as visual phase separation isn't needed. Automated sample preparation allows fast sample process and subsequent analysis. Automated liquid handling coupled with LC-MS/MS as high-throughput method. Washout gradient in LC method removes strongly retaining compounds from the column. Sensitive method of 1.08/1.41 ng/ml for amodiaquine/desethylamodiaquine.

Recent spectrophotometric and electroanalytical methods used for the determination of quinoline-based compounds

“…The solid support, fine porous particles, interacts with the analytes and impurities in an aqueous sample, upon loading them onto SLE particles surface, in an adsorption process. These species are retained by the solid support, then an organic solvent is employed for eluting the target analyte whereas interferants would still adsorb on the support [ 30 ]. It is preferable over SPE and LLE, however, the extraction columns are of single-use, hence rising environmental concern and increasing the analysis cost.…”