High-throughput protein analysis by multiplexed sodium dodecyl sulfate capillary gel electrophoresis with UV absorption detection

Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis

“…Traditionally, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been used for protein separation and molecular weight determination as a qualitative tool for the analysis of seed and food proteins (Luo et al, 2004).…”

Protein separation by capillary gel electrophoresis: A review

“…These matrices had moderate viscosities and could be conveniently replenished. Luo et al [17] performed high-throughput protein analysis by multiplexed SDS-CGE, and Xu et al [37] realized separation and characterization of SDS-protein complexes on a microchip with UV adsorption detection using similar matrices. Hydroxypropyl cellulose (HPC) is another polysaccharide sieving matrix used in CGE.…”

“…The lifetimes of these columns were limited (usually less than 10 runs) [12], and the run-to-run reproducibility was poor. Currently, replaceable and water-soluble linear or slightly branched polymers, such as linear polyacrylamide [11–13], poly(ethylene glycol) [11], poly(ethylene oxide) [14], dextran [15–17], pullulan [18, 19] and cross-linked polyacrylamide [20–22] are used as sieving matrices for CGE [5, 11, 23–25]. Availability of these polymer matrices has led to improved reproducibility and robustness of this methodology.…”

Capillary electrophoresis‐based separation techniques for the analysis of proteins

“…Owing to their self-adsorption capability, the separation of proteins in CE using polymer solutions is commonly conducted in the absence of EOF. Like in SDS-PAGE, SDS is commonly added to polymer solutions to improve resolution and solubility of proteins [151][152][153]. It has been reported that SDS binds to the proteins proportionally to their MW [154] and the charges of the complexes are mainly determined by the bound amount of negatively charged SDS molecules.…”