High-throughput profiling of antibiotic resistance genes in drinking water treatment plants and distribution systems

Xu, Like; Ouyang, Wei-Ying; Qian, Yanyun; Su, Chao; Su, Jian‐Qiang; Hong, Chen

doi:10.1016/j.envpol.2016.02.013

Cited by 262 publications

(109 citation statements)

References 38 publications

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“…As highthroughput qPCR was applied in the study, the total number of 285 genes were searched. As a result, from 112 to 122 genes were found in tap water samples, depicting the diversity of ARGs in this medium [17]. The elevated level of ARGs in tap water, in comparison to treated water, was confirmed also by Zhang et al [51].…”

Section: Args Occurring In Arb and Dna Isolated From Tap Watera Reviewsupporting

confidence: 62%

“…Although the major part of bacteria, including resistant strains, is retained in water treatment plants, the ARGs can enter a water supply system, because the dimensions of genes (which are indeed small DNA fragments) allow them to omit most of the treatment processes, including membrane units [15][16][17]. Moreover, disinfection processes could cause the lysis of bacterial cells in some circumstances, leading to the release of free DNA molecules, which could contain the ARGs [9].…”

Section: Introductionmentioning

confidence: 99%

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The occurrence of antibiotic resistance genes in tap water – a review

Siedlecka

2018

E3S Web Conf.

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Abstract. The study presents a review of the occurrence of genetic determinants of antibiotic resistance in tap water. The aim of this study was also to compare the applied methods for antibiotic resistance genes (ARGs) investigations in tap water. As the concentration of ARGs in treated, drinking water is expected to be very low and may cause problems in a standard isolation procedure, the special emphasis is placed on the applied procedures of DNA extraction and their efficiency. The study presents the first attempts to obtain DNA directly from tap water. Further efforts must be put to determine the final amount of obtained DNA and the presence of chosen ARGs among the molecules.

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Section: Args Occurring In Arb and Dna Isolated From Tap Watera Reviewsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

The occurrence of antibiotic resistance genes in tap water – a review

Siedlecka

2018

E3S Web Conf.

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“…In the absence of access to any data on the type or rate of antibiotic prescription in the community, we decided to look for a subset of genes that have previously been shown to be commonly found in various aquatic environments (35,36). A two-pronged approach to detect various antibiotic resistance genes, a qPCR…”

Section: Discussionmentioning

confidence: 99%

Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Fernando

Tun

Poole

et al. 2016

Appl Environ Microbiol

106

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Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, ␤-lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk. IMPORTANCEIn this study, we highlight the poor microbiological quality of drinking water in a First Nations community in Canada. We examined the coliform load as well as the presence of antibiotic resistance genes in these samples. This study examined the presence of antibiotic-resistant genes in drinking water samples from a First Nations Community in Canada. We believe that our findings are of considerable significance, since the issue of poor water quality in First Nations communities in Canada is often ignored, and our findings will help shed some light on this important issue.A ntibiotic resistance in bacteria has been recognized as one of the greatest threats to human health by the World Health Organization (1). Overuse and misuse of antibiotics contribute to the buildup of selective pressure aiding the proliferation of antibiotic-resistant bacteria (2, 3). While hospital environments are notorious for selecting for antibiotic-resistant bacteria, it is now becoming increasingly evident that overuse and misuse of antibiotics are also creating a selective pressure outside hospital settings. Studies over the last few years have shown the presence of antibiotics and of antibiotic-resistant bacteria in the broader environment, including water supplies and soil samples (4). This is indeed alarming as the high number of antibiotic-resistant bacteria in communities makes the treatment of community-acquired infections increasingly challenging (5, 6).Not surprisingly, water samples from communities that lack access to clean water contain high numbers of bacteria (7-9). While a high bacterial count in the water supply itself poses an increased health risk (10), the presence of antibiotic-resistant bacteria makes this risk even more serious. Lack of access to clean and safe water is a problem that is generally associated with developing countries; however, this is a reality as wel...

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“…These aggregations, including cells, ferrihydrite, and biochar, may accelerate the electron transfer by reducing the distance required for extracellular electron transfer among cells, biochar, and ferrihydrite (Kappler et al, 2014). In addition to the electron shuttling by its active site-quinones, biochar is also capable of binding divalent cations (Mosley et al, 2015) and therefore enhances iron(III) oxide reduction by decreasing and delaying Fe(II) sorption to Fe(III) oxides and Fe(III)-reducing bacteria cell surfaces (Urrutia et al, 1999; Xu et al, 2016). The powdered biochar had a larger surface for Fe(II) sorption than granulated biochar, which potentially contributed to the greater amounts of iron(III) reduction.…”

Section: Discussionmentioning

confidence: 99%

Biochar Addition Increases the Rates of Dissimilatory Iron Reduction and Methanogenesis in Ferrihydrite Enrichments

et al. 2017

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Biochar contains quinones and aromatic structures that facilitate extracellular electron transfer between microbial cells and insoluble minerals. In this study, granulated biochar (1.2–2 mm) and powdered biochar (<0.15 mm) were amended to two ferrihydrite (in situ ferrihydrite and ex situ ferrihydrite) enrichments to investigate the effect of biochar with different particle sizes on dissimilatory iron(III)-reducing bacteria (DIRB) and methanogens. Biochar addition significantly stimulated the reduction of both in situ ferrihydrite and ex situ ferrihydrite and the production of methane. Powdered biochar amendments increased iron reduction compared to granulated biochar amendment in both the in situ ferrihydrite and ex situ ferrihydrite enrichments. However, no significant difference was observed in methane production between the powdered biochar and granulated biochar amendments in the two ferrihydrite enrichments. Analysis of 16S rRNA gene sequences showed that both DIRB and methanogens were enriched after biochar amendments in the in situ ferrihydrite and ex situ ferrihydrite enrichments. Taxa belonging to the Geobacteraceae and methanogenic genus affiliated to Methanosarcina were detected with significantly higher relative abundances in powdered biochar amendments than those in granulated biochar amendments in both the ferrihydrite enrichments. X-ray diffraction analysis indicated green rust [Fe2(CO3) (OH)] and vivianite [Fe3(PO4)2 8(H2O)] formed in the ex situ ferrihydrite and in situ ferrihydrite enrichments without biochar addition, respectively. After granulated biochar amendment, the mineral phase changed from the green rust to vivianite in the ex situ ferrihydrite enrichment, while crystalline vivianite and iron oxide (γ-Fe2O3) were detected simultaneously in the in situ ferrihydrite enrichment. No crystalline iron compound was found in the powdered biochar amendments in both ferrihydrite enrichments. Overall, our study illustrated that the addition of biochar affected iron-reducing and methane-generating microbial communities to some extent.

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High-throughput profiling of antibiotic resistance genes in drinking water treatment plants and distribution systems

Cited by 262 publications

References 38 publications

The occurrence of antibiotic resistance genes in tap water – a review

The occurrence of antibiotic resistance genes in tap water – a review

Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada

Biochar Addition Increases the Rates of Dissimilatory Iron Reduction and Methanogenesis in Ferrihydrite Enrichments

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