The occurrence of antibiotic resistance genes in tap water – a review

Siedlecka, Agata

doi:10.1051/e3sconf/20183001012

Cited by 3 publications

(1 citation statement)

References 64 publications

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“…Antibiotic and antimicrobial resistance (AMR) has resulted in increased morbidity and death as a result of treatment failures, as well as higher health-care expenses [ 1 , 2 ]. Research has it that AMR is expected to become the major cause of death in the world by 2050, with 10 million fatalities every year.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

et al. 2022

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Microorganisms are one of the main sources of antimicrobial agents and over 50% of antibiotics currently used in hospitals are metabolites from microbes. This study aimed to isolate microorganisms from the Dompoase landfill site, Kwame Nkrumah University Physics Garden, Kosiko River, and Ada Foah seashore of Ghana and screen their metabolites for antimicrobial activity. Forty-eight (48) microorganisms were isolated and their metabolites were screened against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Vibrio cholerae, Salmonella typhi, Pseudomonas aeruginosa, Streptococcus pyogenes, Proteus mirabilis, and Candida albicans using the agar well diffusion method. Ten (10) of the isolates exhibited antimicrobial activity. Isolate DO5, identified as P. aeruginosa isolate, from the Dompoase landfill site was selected for fermentation because it exhibited the highest activity against all the test organisms. DO5 produced optimum antimicrobial activity when fermented for 11 days at 30°C. In the agar diffusion method, the extract of isolate DO5 recorded zones of inhibition ranging between 11.67 ± 0.23 and 21.50 ± 0.71 mm. The MIC and MBC recorded for the DO5 extract ranged from 3.13–25.0 mg/mL and from 6.25–50.0 mg/mL, respectively. Column chromatography analysis yielded eight (8) subfractions from the DO5 extract. IR analysis revealed the presence of functional groups such as alcohols, esters, and hydrocarbons in the fractions. GC-MS analysis identified nine compounds that have been reported to have antimicrobial agents. The DO5 metabolites stand the chance to be developed into potent antibiotics for infection treatment.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

et al. 2022

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Large-scale pattern of resistance genes and bacterial community in the tap water along the middle and low reaches of the Yangtze River

Zhang

Suyamud

Lohwacharin

et al. 2021

Ecotoxicology and Environmental Safety

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Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp.

Alzahrani¹

2023

J. Pure Appl. Microbiol.

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Recently, the search for novel products derived from natural sources has become necessary due to the decreasing effectiveness of current antibiotics in treating bacteria that are antibiotic-resistant. In this context, it is well known that entomopathogenic bacteria (EPBs) produce a broad range of secondary metabolites with antibacterial activities. Therefore, an in-vitro trial was conducted to isolate and identify non-symbiotic bacteria associated with entomopathogenic nematodes, Steinernema spp. and evaluate the antibacterial activity against four antibiotic-resistant bacteria. Four bacterial isolates, i.e. Pseudomonas alcaligenes ST-1, Paenibacillus barcinonensis ST-2, Bacillus mojavensis ST-3, and Bacillus megaterium ST-4 were non-symbiotically isolated from the heamolymph of dead Steinernema-infected Galleria mellonella larvae and molecularly characterized. The bacterial cells and filtrates from P. alcaligenes ST-1 strongly inhibited the growth of Staphylococcus aureus through disk diffusion (43 mm), minimum inhibitory concentration (2.5 µL/mL), and minimal bactericidal concentration (5 µL/mL) assay. Conclusively, the direct application of endogenous Steinernema-associated EPB as an antibacterial agent for antibiotic-resistant bacteria looked promising.

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The occurrence of antibiotic resistance genes in tap water – a review

Cited by 3 publications

References 64 publications

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

Large-scale pattern of resistance genes and bacterial community in the tap water along the middle and low reaches of the Yangtze River

Isolation, Identification and Antimicrobial Activities of Four Bacteria Associated with Entomopathogenic Nematodes, Steinernema spp.

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