High-throughput planar solid-phase extraction coupled to orbitrap high-resolution mass spectrometry via the autoTLC-MS interface for screening of 66 multi-class antibiotic residues in food of animal origin

Mehl, Annabel; Schmidt, Li S.; Schmidt, Laszlo; Morlock, Gertrud E.

doi:10.1016/j.foodchem.2021.129211

Cited by 37 publications

(30 citation statements)

References 36 publications

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“…Hence, a simpler solution was targeted. Sample preparation could be performed on the surface of the adsorbent [ 29 , 30 ]; however, the high saccharide content might restrict the sample load. This challenge was explored exemplarily for three different monofloral honeys, i.e., Becium grandiflorum , Coffea arabica , and V ernonia amygdalina honeys.…”

Section: Resultsmentioning

confidence: 99%

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

et al. 2022

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Ethiopian honey is used not only as food but also for treatment in traditional medicine. For its valorization, bioactive compounds were analyzed in nine types of monofloral Ethiopian honey. Therefore, a non-target effect-directed profiling was developed via high-performance thin-layer chromatography combined with multi-imaging and planar effect-directed assays. Characteristic bioactivity profiles of the different honeys were determined in terms of antibacterial, free-radical scavenging, and various enzyme inhibitory activities. Honeys from Hypoestes spp. and Leucas abyssinica showed low activity in all assays. In contrast, others from Acacia spp., Becium grandiflorum, Croton macrostachyus, Eucalyptus globulus, Schefflera abyssinica, Vernonia amygdalina, and Coffea arabica showed more intense activity profiles, but these differed depending on the assay. In particular, the radical scavenging activity of Croton macrostachyus and Coffea arabica honeys, the acetylcholinesterase-inhibiting activity of Eucalyptus globulus and Coffea arabica honeys, and the antibacterial activity of Schefflera abyssinica honey are highlighted. Bioactive compounds of interest were further characterized by high-resolution mass spectrometry. Identifying differences in bioactivity between mono-floral honey types affects quality designation and branding. Effect-directed profiling provides new insights that are valuable for food science and nutrition as well as for the market, and contributes to honey differentiation, categorization, and authentication.

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Section: Resultsmentioning

confidence: 99%

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

et al. 2022

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“…The analogous hyphenation was performed, however, using the open-source modified autoTLC–LC–MS, which was fully automated for sequential analysis, connected via a monolithic column (Chromolith Flash RP-18e, 25 × 2 mm, Merck, Darmstadt, Germany) to a high-resolution mass spectrometry (UHPLC–PDA–HESI–HRMS) system with a PDA detector and a Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) …”

Section: Methodsmentioning

confidence: 99%

Effects of the Probiotic Activity of Bacillus subtilis DSM 29784 in Cultures and Feeding Stuff

Kruse

Pierre²,

Morlock

2021

J. Agric. Food Chem.

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The European Union banned the usage of antibiotic growth promoters in animal production. The probiotic microorganism of the genus Bacillus appeared to be an attractive candidate to replace antibiotics. The Bacillus subtilis DSM 29784 is one of these strains. To date, the probiotic effect has not been completely understood, but it is supposed that the effect depends on metabolites of the microorganism. Imaging high-performance thin-layer chromatography (HPTLC) is a powerful tool to visualize differences in the metabolite profile of bacteria with high genetic similarity to allow a better understanding of the probiotic effect. In comparison to other bacteria, especially these bacterial cells were more robust to harsh cultivation conditions and produced a higher level of antioxidants or bioactive substances such as surfactin. HPTLC enabled the comparison of pure cell cultures to the spore cultivation in the feed, and the results explain and support the probiotic effect.

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“…The limit of quantitative determination of antibiotics was from 0.5 to 100 μg/L. A. Mehl et al [26] developed a high-throughput planar solid-phase extraction method to rapidly screen nine different classes of antibiotics (sulfonamides, diaminopyrimidines, lincosamides pleuromutilins, macrolides, cephalosporins, penicillins, amphenicols and nitroimidazoles). Meat, cow's milk and chicken eggs were used as research objects.…”

Section: Sulfonamidesmentioning

confidence: 99%

“…According to 2002/657/EC, the test method is approved for one antibiotic of each class. The authors suggest that the analysis method developed by them will increase food safety by studying samples [26].…”

Section: Sulfonamidesmentioning

confidence: 99%

Untitled

2022

J. Med. Chem. Sci.

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Due to the widespread use of veterinary drugs and antibiotics in animal husbandry, one of the most critical problems in ensuring food safety is the presence of residual antibiotic traces in animal products. Concentrations of such traces in food products pose a danger to both humans and the environment as a whole. Irrational use of antibiotics in agriculture stimulates resistant bacteria that can cause infectious diseases in humans and animals that cannot be treated with modern drugs. Due to the potential risk to human health in many countries, maximum permissible limits for the content of residual traces of antibiotics are regulated. This review aimed at investigating the recent publications in the field of identification of residual antibiotic traces in food. Various types of high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) combined with solid-phase extraction were mainly used for the quantitative determination of analytes. Methods of enzyme immunoassay, capillary electrophoresis, and infrared spectroscopy with Fourier transform, biosensor, and fluorescence analysis methods are also presented. It is shown that the development of new susceptible, accurate, simple, and cost-effective methods for the determination traces of antibiotics in food remains an urgent task and is highly demanded in the modern world.

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High-throughput planar solid-phase extraction coupled to orbitrap high-resolution mass spectrometry via the autoTLC-MS interface for screening of 66 multi-class antibiotic residues in food of animal origin

Cited by 37 publications

References 36 publications

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

Effect-Directed Profiling of Monofloral Honeys from Ethiopia by High-Performance Thin-Layer Chromatography and High-Resolution Mass Spectrometry

Effects of the Probiotic Activity of Bacillus subtilis DSM 29784 in Cultures and Feeding Stuff

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