The
European Union banned the usage of antibiotic growth promoters
in animal production. The probiotic microorganism of the genus Bacillus appeared to be an attractive candidate to
replace antibiotics. The Bacillus subtilis DSM 29784 is one of these strains. To date, the probiotic effect
has not been completely understood, but it is supposed that the effect
depends on metabolites of the microorganism. Imaging high-performance
thin-layer chromatography (HPTLC) is a powerful tool to visualize
differences in the metabolite profile of bacteria with high genetic
similarity to allow a better understanding of the probiotic effect.
In comparison to other bacteria, especially these bacterial cells
were more robust to harsh cultivation conditions and produced a higher
level of antioxidants or bioactive substances such as surfactin. HPTLC
enabled the comparison of pure cell cultures to the spore cultivation
in the feed, and the results explain and support the probiotic effect.
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