High-Throughput Nuclear Magnetic Resonance Analysis Using a Multiple Coil Flow Probe

Abstract

An automated method for high-throughput nuclear magnetic resonance (NMR) spectroscopy has been developed using a four-coil Multiplex NMR probe. The probe is constructed with solenoidal microcoils optimized for detection of small volume, mass-limited samples and a flow-through design. Four samples can be simultaneously injected into the Multiplex probe with a robotics liquid handler and then analyzed in rapid succession using a selective excitation experiment. Due to the simultaneous injection of four samples and the reduced analysis time with rapid selective excitation, the analysis rate achieved thus far is as low as 1 sample/34 s for 1D 1H NMR.

Time as a Dimension in High‐Throughput Homogeneous Catalysis

“…Emerging methods for multiplexed GC can handle up to 450 samples/h. [21] Comparably impressive throughput has been described in flow NMR [22,23] and capillary electrophoresis [24,25] in enantioselectivity determination.…”

Design and construction of a microcoil NMR probe for the routine analysis of 20‐μL samples

“…The current SNR of this probe is diminished compared with the commercially available small volume microcoil probes (3,25,38) or those previously constructed in our laboratory (10,13,16). We believe that a significant loss of signal results from the use of the transmission line element.…”

“…The configuration permits the interface of an automated liquids handler for increased throughput analyses of routine samples from 96-well plates (13). Similar work has resulted in multisample probes of up to eight coils (14).…”

High-throughput NMR spectroscopy

“…An alternative method combines separate detection coils connected in parallel with magnetic field gradients used to separate the signals from each sample [16,17]. Throughput rates as high as two samples per minute have been achieved to date [18].…”