High throughput molecularly imprinted polymers based electrochemical nanosensors for point-of-care diagnostics of COVID-19

Singhal, Ayushi; Parihar, Arpana; Kumar, Neeraj; Khan, Raju

doi:10.1016/j.matlet.2021.130898

Cited by 46 publications

(28 citation statements)

References 18 publications

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“…It is noted that the 3D molecular-imprinted sensor shares similarities with the recently reported electrochemical biosensor based on a molecularly imprinted polymer (MIP), which could effectively detect SARS-CoV-2 S-protein and an antigen with high throughput. 30 − 32 The MIP sensor utilizes the molecular imprint of target molecules created on the polymer via in situ polymerization, rather than the crystallized SAM used in the current 3D molecular-printed sensor, which can detect both molecules and entire virions by controlling the roughness of the underlying substrate. 29 …”

Section: Resultsmentioning

confidence: 99%

Potentiometric Biosensors Based on Molecular-Imprinted Self-Assembled Monolayer Films for Rapid Detection of Influenza A Virus and SARS-CoV-2 Spike Protein

Lee

Subramanian

Mueller

et al. 2022

ACS Appl. Nano Mater.

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Rapid, yet accurate and sensitive testing has been shown to be critical in the control of spreading pandemic diseases such as COVID-19. Current methods which are highly sensitive and can differentiate different strains are slow and cannot be conveniently applied at the point of care. Rapid tests, meanwhile, require a high titer and are not sufficiently sensitive to discriminate between strains. Here, we report a rapid and facile potentiometric detection method based on nanoscale, three-dimensional molecular imprints of analytes on a self-assembled monolayer (SAM), which can deliver analyte-specific detection of both whole virions and isolated proteins in microliter amounts of bodily fluids within minutes. The detection substrate with nanoscale inverse surface patterns of analytes formed by a SAM identifies a target analyte by recognizing its surface nano- and molecular structures, which can be monitored by temporal measurement of the change in substrate open-circuit potential. The sensor unambiguously detected and differentiated H1N1 and H3N2 influenza A virions as well as the spike proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Middle-East respiratory syndrome (MERS) coronavirus in human saliva with limits of detection reaching 200 PFU/mL and 100 pg/mL for the viral particles and spike proteins, respectively. The demonstrated speed and specificity of detection, combined with a low required sample volume, high sensitivity, ease of potentiometric measurement, and simple sample collection and preparation, suggest that the technique can be used as a highly effective point-of-care diagnostic platform for a fast, accurate, and specific detection of various viral pathogens and their variants.

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Section: Resultsmentioning

confidence: 99%

Potentiometric Biosensors Based on Molecular-Imprinted Self-Assembled Monolayer Films for Rapid Detection of Influenza A Virus and SARS-CoV-2 Spike Protein

Lee

Subramanian

Mueller

et al. 2022

ACS Appl. Nano Mater.

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show abstract

“…In the precursor MAX phase, the etching procedure is primarily used to disrupt the M–A metal bond. Fluorine-containing acid etching [ 34 , 147 ], halogen etching [ 148 ], strong alkaline etching [ 149 ], high-temperature etching [ 150 ], and electrochemical etching [ 151 ] are the different types of etching methods used so far. The most popular technique for etching the A layer is to use a hydrofluoric acid (HF) or a strong alkali.…”

Section: Mxene: Synthesis and Propertiesmentioning

confidence: 99%

“…The excellent electrical, mechanical, electrochemical, and optical features of 2 D materials have fetched much attention from the past decade in terms of their wide applicability in various fields including disease diagnosis and therapeutics [ 25 ]. There are several types of 2D materials such as graphitic carbon nitride [ 26 ], transition metal dichalcogenides [ 27 ], black phosphorous [ 28 ], hexagonal boron nitride (borophene) [ 29 ], graphene [ 30 ], metal halides [ 31 ], metal oxides [ 32 ], metal–organic frameworks [ 33 ], some polymer [ 34 ] which have been investigated for various biosensing applications. Among these, early transition metal carbides and/or nitrides (MXene) are unique in terms of their hydrophilicity, electrochemical, mechanical, and optical properties [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Next-Generation Intelligent MXene-Based Electrochemical Aptasensors for Point-of-Care Cancer Diagnostics

et al. 2022

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Delayed diagnosis of cancer using conventional diagnostic modalities needs to be addressed to reduce the mortality rate of cancer. Recently, 2D nanomaterial-enabled advanced biosensors have shown potential towards the early diagnosis of cancer. The high surface area, surface functional groups availability, and excellent electrical conductivity of MXene make it the 2D material of choice for the fabrication of advanced electrochemical biosensors for disease diagnostics. MXene-enabled electrochemical aptasensors have shown great promise for the detection of cancer biomarkers with a femtomolar limit of detection. Additionally, the stability, ease of synthesis, good reproducibility, and high specificity offered by MXene-enabled aptasensors hold promise to be the mainstream diagnostic approach. In this review, the design and fabrication of MXene-based electrochemical aptasensors for the detection of cancer biomarkers have been discussed. Besides, various synthetic processes and useful properties of MXenes which can be tuned and optimized easily and efficiently to fabricate sensitive biosensors have been elucidated. Further, futuristic sensing applications along with challenges will be deliberated herein.

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“…MIP biosensors have an excellent potential due to their long-term and thermal stability, cost and high specificity and stability; however, they present certain limitations related to the clustering of nanomaterials during synthesis. Nevertheless, the commercialization of MIP biosensors is still limited [151]. The potential electrochemical immunosensors for the fast testing of SARS-CoV-2 was analyzed by Ranjan et al [152].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Point-of-Care Testing—The Key in the Battle against SARS-CoV-2 Pandemic

et al. 2021

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The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. Currently, the “gold standard” involves the use of quantitative real-time polymerase chain reaction (qRT-PCR) for SARS-CoV-2 detection. Even though it is sensitive, specific and applicable for large batches of samples, qRT-PCR is labour-intensive, time-consuming, requires trained personnel and is not available in remote settings. This review summarizes and compares the available strategies for COVID-19: serological testing, Point-of-Care Testing, nanotechnology-based approaches and biosensors. Last but not least, we address the advantages and limitations of these methods as well as perspectives in COVID-19 diagnostics. The effort is constantly focused on understanding the quickly changing landscape of available diagnostic testing of COVID-19 at the clinical levels and introducing reliable and rapid screening point of care testing. The last approach is key to aid the clinical decision-making process for infection control, enhancing an appropriate treatment strategy and prompt isolation of asymptomatic/mild cases. As a viable alternative, Point-of-Care Testing (POCT) is typically low-cost and user-friendly, hence harbouring tremendous potential for rapid COVID-19 diagnosis.

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High throughput molecularly imprinted polymers based electrochemical nanosensors for point-of-care diagnostics of COVID-19

Abstract: Graphical abstract

Cited by 46 publications

References 18 publications

Potentiometric Biosensors Based on Molecular-Imprinted Self-Assembled Monolayer Films for Rapid Detection of Influenza A Virus and SARS-CoV-2 Spike Protein

Potentiometric Biosensors Based on Molecular-Imprinted Self-Assembled Monolayer Films for Rapid Detection of Influenza A Virus and SARS-CoV-2 Spike Protein

Next-Generation Intelligent MXene-Based Electrochemical Aptasensors for Point-of-Care Cancer Diagnostics

Point-of-Care Testing—The Key in the Battle against SARS-CoV-2 Pandemic

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