High-throughput LC-MS/MS method for monitoring sirolimus and everolimus in the routine clinical laboratory

Abstract

The findings made by us indicate that the proposed method is of value, since it is speedy, straightforward, accurate, and applicable to different LC-MS/MS instruments for the routine TDM of organ transplant recipients.

Therapeutic drug monitoring of immunosuppressants by liquid chromatography–mass spectrometry

“…Since LC-MS/MS is not dependent on antibodies, which suffer from poor specificity with similar small molecules, methods can be rapidly developed to quantify these analytes. The increased specificity of LC-MS/MS was exploited in several recent papers to simultaneously measure sirolimus and everolimus [33,34]. These methods were adapted to avoid LC column heating, which can lead to short column lifespans, in another study [35].…”

Antineoplastic drugs and their analysis: a state of the art review

“…589 The sensitivity and selectivity provided by MS helped to ease sample preparation in clinical chemistry laboratories. Column-switching systems coupling the extraction support to the chromatographic column before MS detection were also used for the analysis of sirolimus [598][599][600][601][602][603][604][605][606][607][608][609][610][611] and everolimus 598,599,602,603,[605][606][607]609,[612][613][614] in whole blood. LLE 615 or SPE 616 on 96-well plates constitutes another approach for sample preparation automatisation.…”

Laboratory medicine and medical oncology: the tale of two Cinderellas

“…Monitoring of serum concentrations may also be useful in cancer patients treated with targeted agents. Highly sensitive methods have been developed for a number of agents, e.g., mammalian targeted of rapamycin inhibitors [117] . However, monitoring of therapeutic drug concentrations has still not entered routine use in medical oncology.…”