High-throughput HPLC-MS/MS method to determine ibandronate in human plasma for pharmacokinetic applications

Tarcomnicu, Isabela; Gheorghe, Mihaela; Silvestro, Luigi; Savu, Simona Rizea; Boaru, Irina; Tudoroniu, Ariana

doi:10.1016/j.jchromb.2009.08.007

Cited by 29 publications

(28 citation statements)

References 18 publications

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“…Thus, more sensitive and specific quantitative methods such as LC-MS/MS-based assays would be of interest as shown in recent publications for the sensitive quantification of alendronate and ibandronate in human urine and plasma [18,19].…”

Section: Introductionmentioning

confidence: 99%

LC–MS/MS method for the determination of clodronate in human plasma

Hasan

Schumacher

Seekamp

et al. 2014

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

LC–MS/MS method for the determination of clodronate in human plasma

Hasan

Schumacher

Seekamp

et al. 2014

Journal of Pharmaceutical and Biomedical Analysis

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“…With the help of a switching valve 2 columns were run in parallel, resulting in an analysis time of 5 min/sample, that was very short compared with HPLC-fluorescence or with GC methods. Trimethylsilyl diazomethane (TMSD) was another derivatization reagent successfully used in LC-MS/MS, in order to analyze ibandronate from plasma samples for pharmacokinetic applications (Tarcomnicu et al, 2009). A high-throughput method with an LLOQ of 200 pg/mL was optimized and fully validated according to FDA guidelines.…”

Section: Overview On Mass Spectrometric Analysis Of Biphosphonatesmentioning

confidence: 99%

HPLC-MS/MS of Highly Polar Compounds

Silvestro¹,

Tarcomnicu²,

Savu³

2012

Tandem Mass Spectrometry - Applications and Principles

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“…Due to the complex matrix of human urine, direct CE-ECL analysis of IBAN in urine was difficult. Tedious and time-consuming pretreatment of biological materials was always involved prior to IBAN analysis [4,6,7]. In this study, we attempted to develop a simple SPE procedure for IBAN in urine sample.…”

Section: Analysis Of Human Urinementioning

confidence: 99%

“…The LOD of IBAN was 72 nM ($23 ng/mL). A high-throughput HPLC-MS/MS method for determining IBAN in human plasma has been developed by Tarcomnicu et al [7]. Liquid-liquid extraction and methylation derivatization were used in sample preparation.…”

Section: Introductionmentioning

confidence: 99%

“…The electropherogram of the same urine sample subjected to ultrafiltration followed by SPE with are more than three orders of magnitude lower than those of HPLC-ELSD [5] and IC conductometry [8] methods, and are similar to that of the technically complicated HPLC-photochemical derivatization-fluorometric detection method [6]. Although GC-MS [4] and HPLC-MS/MS [7] methods could provide lower ng/mL level LOQ for IBAN in biological fluids, both analyte derivatization and high-cost instrumentation were required. ELISA is the most sensitive and selective method for IBAN reported to date.…”

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confidence: 99%

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Capillary electrophoresis–electrochemiluminescence detection method for the analysis of ibandronate in drug formulations and human urine

2011

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A simple, rapid and sensitive CE method coupled with electrochemiluminescence (ECL) detection for direct analysis of ibandronate (IBAN) has been developed. Using a buffer solution of 20 mM sodium phosphate (pH 9.0) and a voltage of 13.5 kV, separation of IBAN in a 30-cm length capillary was achieved in 3 min. ECL detection was performed with an indium tin oxide working electrode bias at 1.6 V (versus a Pt wire reference) in a 200-mM sodium phosphate buffer (pH 8.0) containing 3.5 mM Ru(bpy)(3)(2+) (where bpy=2,2'-bipyridyl). Derivatization of IBAN prior to CE-ECL analysis was not needed. Linear correlation (r=0.9992, n=7) between ECL intensity and analyte concentration was obtained in the range of 0.25-50 μM IBAN. The LOD of IBAN in water was 0.08 μM. The developed method was applied to the analysis of IBAN in a drug formulation and human urine sample. SPE using magnetic Fe(3)O(4)@Al(2)O(3) nanoparticles as the extraction phase was employed to pretreat the urine sample before CE-ECL analysis. The linear range was 0.2-12.0 μM IBAN in human urine (r=0.9974, n=6). The LOD of IBAN in urine was 0.06 μM. Total analysis time including sample preparation was <1 h.

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High-throughput HPLC-MS/MS method to determine ibandronate in human plasma for pharmacokinetic applications

Cited by 29 publications

References 18 publications

LC–MS/MS method for the determination of clodronate in human plasma

LC–MS/MS method for the determination of clodronate in human plasma

HPLC-MS/MS of Highly Polar Compounds

Capillary electrophoresis–electrochemiluminescence detection method for the analysis of ibandronate in drug formulations and human urine

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