High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer

Wakao, Osamu; Satou, Ken; Nakamura, Ayano; Galkina, Polina A.; Nishiyama, Keine; Sumiyoshi, Ken; Kurosawa, Fumio; Maeki, Masatoshi; Ishida, Akihiko; Tani, Hirofumi; Проскурнин, М. А.; Shigemura, Koji; Hibara, Akihide

doi:10.1039/c9lc00256a

Cited by 21 publications

(14 citation statements)

References 26 publications

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“…NC-FPIA was demonstrated using a portable FPIA analyzer with a microfluidic device (Fig. S1) [15,16]. Antigen (purified virus or H5-rHA) in PBS, 80 ng/mL F-Fab, and 1% BSA in PBS were added to a microtube.…”

Section: Assay Proceduresmentioning

confidence: 99%

“…Most applications of conventional competitive FPIA have been for quantifying small molecules such as drugs and mycotoxins [13]. Using the advantages of FPIA, we have developed a portable FPIA analyzer that can measure multiple samples simultaneously on site with a small amount of sample [14][15][16]. In addition, recently, we have demonstrated non-competitive FPIA (NC-FPIA) using a nanobody [17] or a Fab fragment [18] for proteins to expand the applications of FPIA.…”

Section: Introductionmentioning

confidence: 99%

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Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer

et al. 2021

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Nowadays, the diagnosis of viral infections is receiving broad attention. We have developed a non-competitive fluorescence polarization immunoassay (NC-FPIA), which is a separation-free immunoassay, for a virus detection. H5 subtype avian influenza virus (H5-AIV) was used as a model virus for the proof of concept. The fluorescein-labeled Fab fragment that binds to H5 hemagglutinin was used for NC-FPIA. The purified H5-AIV which has H5 hemagglutinin was mixed with the fluorescein-labeled Fab fragment. After that, the degree of fluorescence polarization was measured with a portable FPIA analyzer. H5-AIV was successfully detected with an incubation time of 15 min. In addition, the portable FPIA analyzer enables performance of on-site NC-FPIA with a sample volume of 20 μL or less. This is the first research of detecting a virus particle by FPIA. This NC-FPIA can be applied to rapid on-site diagnosis of various viruses. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03193-y.

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Section: Assay Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer

et al. 2021

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“…FPIA is usually performed using stationary optical technology. In this regard, there is demand for recent development [14], in which a portable detection for fluorescence polarization measurements was proposed and characterized. By this way, FPIA was carried out using a portable FP analyzer (the dimensions of the device are 35 × 15 × 15 cm).…”

Section: Instrument Base For Fluorescence Polarization Measurementsmentioning

confidence: 99%

Bioanalytical Technologies for Safety Control of Fish and Seafood by Sensitive Rapid Tests for Phycotoxins

Eremin

Zherdev

Tokeshi

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Wide and operate safety monitoring of foodstuffs is highly demanded in modern society. This work aims to develop and characterize new test systems for control of phycotoxins, dangerous contaminants of fish and seafood. For rapid and productive testing, 2 methods have been implemented: immunochromatographic assay and fluorescence polarization immunoassay. Various approaches for reducing the limit of detection of target analytes have been considered, including changes in the use of optical and fluorescent labels and varied order of the detected complexes formation. Antibodies and aptamers have been considered as receptor molecules. The developed techniques provide rapid (20–30 minutes, including sample preparation) and sensitive testing. Their effectiveness has been shown for different kinds of fish and seafood. Portable detectors have been proposed that allow testing directly at the sampling points, without transportation to centralized laboratories.

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“…Compared to heterogeneous fluorescence assays, homogeneous assays such as fluorescence anisotropy or polarization‐based ones have a better potential for high‐throughput screening that can avoid the immobilization, separation, or washing steps. Wakao et al has innovated a fluorescence polarization analyzer embedded with a microfluidic chip as the sample holder to achieve a high‐throughput screening . In their design, they used microfluidics instead of conventional optical components to achieve the portability and reduce the cost.…”

Section: Microarray‐based Fluorescent/luminescent Readoutmentioning

confidence: 99%

Microfluidics‐Implemented Biochemical Assays: From the Perspective of Readout

Chen

Shao

Xianyu

2019

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Over the past decades, microfluidics has emerged as an increasingly important tool to perform biochemical assays for diagnosis and healthcare. The precise fluid control and molecule manipulation within microfluidics greatly contribute to developing assays with simplicity and convenience. The advantages of microfluidics, including decreased consumption of reagents and samples, lower operating and analysis time, much lower cost, and higher integration and automation over traditional systems, offer a great platform to meet the needs of point‐of‐care applications. In this Review, versatile strategies are outlined and recent advances in microfluidics‐implemented assays are discussed from the perspective of readout, because a convenient and straightforward readout is what a biochemical assay requires and the end user desires. Functions and properties arising from each readout are reviewed and the advantages and limitations of each readout are discussed together with current challenges and future perspectives.

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High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer

Abstract: A portable fluorescence polarization imaging analyzer was developed for high-throughput fluorescence polarization immunoassay.

Cited by 21 publications

References 26 publications

Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer

Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer

Bioanalytical Technologies for Safety Control of Fish and Seafood by Sensitive Rapid Tests for Phycotoxins

Microfluidics‐Implemented Biochemical Assays: From the Perspective of Readout

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