Bioanalytical Technologies for Safety Control of Fish and Seafood by Sensitive Rapid Tests for Phycotoxins

Abstract

Wide and operate safety monitoring of foodstuffs is highly demanded in modern society. This work aims to develop and characterize new test systems for control of phycotoxins, dangerous contaminants of fish and seafood. For rapid and productive testing, 2 methods have been implemented: immunochromatographic assay and fluorescence polarization immunoassay. Various approaches for reducing the limit of detection of target analytes have been considered, including changes in the use of optical and fluorescent labels and varied order of the detected complexes formation. Antibodies and aptamers have been considered as receptor molecules. The developed techniques provide rapid (20–30 minutes, including sample preparation) and sensitive testing. Their effectiveness has been shown for different kinds of fish and seafood. Portable detectors have been proposed that allow testing directly at the sampling points, without transportation to centralized laboratories.