High-throughput detection of food-borne pathogenic bacteria using oligonucleotide microarray with quantum dots as fluorescent labels

Qiu, Zhigang; Jin, Min; Shen, Zhiqiang; Chen, Zhaoli; Wang, Xinwei; Lǐ, Jùnwén

doi:10.1016/j.ijfoodmicro.2014.05.012

Cited by 45 publications

(14 citation statements)

References 47 publications

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“…As a novel fluorescent tag, QDs or CDs are conjugated to recognition elements (antibody, aptamer, or phage), which then bind with bacterial cells (11,28,119,131,132), toxins (95), or DNA (16,47) for detection by fluorescence microscopy (130), flow cytometry (26,130), microarrays (47), or electrochemiluminescence (129). S. aureus (16), Salmonella Enteritidis (28), Escherichia coli (28,103,115), and Salmonella Typhimurium (119,131) have been detected in various food matrices using QDlabeled antibodies.…”

Section: Detection Methods Based On Nanomaterialsmentioning

confidence: 99%

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen

Park

2016

Journal of Food Protection

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Bacterial pathogens are one of the leading causes of food safety incidents and product recalls worldwide. Timely detection and identification of microbial contamination in agricultural and food products is crucial for disease prevention and outbreak investigation. In efforts to improve and/or replace time-consuming and laborious "gold standards" for pathogen detection, numerous alternative rapid methods have been proposed in the past 15 years, with a trend toward incorporating nanotechnology and nanomaterials in food pathogen detection. This article is a review of the use of nanotechnology in various detection and sample preparation techniques and advancements in nanotechnology applications in food matrices. Some practical considerations in nanobioassay design are discussed, and the gaps between research status quo and market demands are identified.

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Section: Detection Methods Based On Nanomaterialsmentioning

confidence: 99%

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen

Park

2016

Journal of Food Protection

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“…Bacterial identification by microarray has been demonstrated for potential biothreat organisms, such as Bacillus anthracis, where reliable differentiation from environmental Bacillus species is necessary (Burton, Oshota, & Silman, 2006) and for food or waterborne pathogenic bacteria (Huang et al, 2014;Maynard et al, 2005;Zhou et al, 2011). Substantial genotype information can be derived from these isolates at a single-nucleotide polymorphism (SNP) level (Thierry et al, 2013).…”

Section: Identification Of Organisms Isolated By Culture-based Methodsmentioning

confidence: 99%

Solid and Suspension Microarrays for Microbial Diagnostics

Miller

Karaöz

Brodie

et al. 2015

Methods in Microbiology

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“…EM enhancement can achieve a SERS EF of ∼10 10 . This indicates that EM is much larger than chemical enhancement.…”

Section: B3100mentioning

confidence: 99%

“…For example, oligonucleotide microarray with combination of CdSe/ZnS quantum dots as fluorescent labels shown high specificity and sensitivity of 10 colony forming units (CFU)/mL. 10 However, the complicated procedures are very time-consuming, and the method is also vulnerable to contamination. These laboratory-based techniques cannot meet the critical need of rapid on-site measurement of pollutants.…”

mentioning

confidence: 99%

Review—Surface-Enhanced Raman Scattering Sensors for Food Safety and Environmental Monitoring

Tang

Zhu

Meng

et al. 2018

J. Electrochem. Soc.

153

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This article gives a review of surface-enhanced Raman scattering (SERS) sensors for food safety and environmental pollution monitoring. It introduces the basic concepts of SERS substrates, SERS enhancement factors and mechanisms, SERS probes/labels, molecular recognition elements as well as optofluidic SERS devices (SERS sensors integrated with microfluidics). This article places an emphasis on the design strategies of various SERS sensors by utilizing fingerprinting SERS spectra or by using SERS probes. It highlights the applications of SERS sensors in detection of heavy metals (such as Hg 2+ , Pb 2+ , Cd 2+ and As 3+ ), inorganic anions (nitrite, nitrate, perchlorate and fluoride), toxic small molecules (polycyclic aromatic hydrocarbon, polychlorinated biphenyls, herbicides, pesticides, antibiotics and food additives), as well as pathogens (bacteria and viruses Food safety and environment pollution are among great challenges in the human society. There are some common pollutants in the environment and food, such as heavy metals, nitrites, phosphates, pesticides, herbicides, antibiotics, hormone, pathogens and other additives. Some pollutants initially are released into the environment, and then accumulate in the food chain, and enter human bodies. Uptake of pollutants by human poses a threat to human health to different extents. 1,2Hence it is essential to monitor pollutants in the environment and food. Currently pollutants are typically measured with various laboratorybased techniques, such as atomic absorption spectroscopy, atomic fluorescence spectrometry, X-ray fluorescence spectrum, inductively coupled plasma and ion-coupled plasma-mass spectroscopy, mass spectrometry, chromatography, capillary electrophoresis, analytical profile index (API), polymerase chain reaction (PCR), and enzymelinked immunosorbent assay (ELISA).3-7 Although these techniques have high sensitivity and accuracy, they rely on expensive instruments and require professional to treat samples and operate instruments in centralized laboratories. Additionally, analysis generally takes several hours to days. For example, 0.024 ppb of Hg 2+ and 0.023 ppb of As 3+ in drinking water can be detected using atomic absorption spectroscopy with a two-step electrothermal atomizer and hydride generation atomic fluorescence spectrometry, respectively. 8,9 However, they need multiple sample preconcentration processing and expensive instruments. PCR can improve the number of the target while ELISA can produce amplified recognition signal, which could both improve the detection sensitivity to pathogens. For example, oligonucleotide microarray with combination of CdSe/ZnS quantum dots as fluorescent labels shown high specificity and sensitivity of 10 colony forming units (CFU)/mL. 10 However, the complicated procedures are very time-consuming, and the method is also vulnerable to contamination. These laboratory-based techniques cannot meet the critical need of rapid on-site measurement of pollutants. Therefore, it is essential to develop portable sensors f...

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High-throughput detection of food-borne pathogenic bacteria using oligonucleotide microarray with quantum dots as fluorescent labels

Cited by 45 publications

References 47 publications

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Solid and Suspension Microarrays for Microbial Diagnostics

Review—Surface-Enhanced Raman Scattering Sensors for Food Safety and Environmental Monitoring

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