High-throughput cell and spheroid mechanics in virtual fluidic channels

Panhwar, Muzaffar H.; Czerwinski, Fabian; Dabbiru, Venkata A. S.; Komaragiri, Yesaswini; Fregin, Bob; Biedenweg, Doreen; Nestler, Peter; Pires, Ricardo H.; Otto, Oliver

doi:10.1038/s41467-020-15813-9

Cited by 35 publications

(20 citation statements)

References 63 publications

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“…Sizes reported in RT-FDC measurements are based on the projected cross-sectional area of the deformed cells. Simulations of the flow fields inside the channel suggest that the stresses near the centerline can be approximated as axisymmetric ( Panhwar et al, 2020 ). Therefore, it is fair to assume that the RBCs take up an axisymmetric shape as well and the projected area is directly correlated to cell size.…”

Section: Discussionmentioning

confidence: 99%

Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium

et al. 2022

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Misshaped red blood cells (RBCs), characterized by thorn-like protrusions known as acanthocytes, are a key diagnostic feature in Chorea-Acanthocytosis (ChAc), a rare neurodegenerative disorder. The altered RBC morphology likely influences their biomechanical properties which are crucial for the cells to pass the microvasculature. Here, we investigated blood cell deformability of five ChAc patients compared to healthy controls during up to 1-year individual off-label treatment with the tyrosine kinase inhibitor dasatinib or several weeks with lithium. Measurements with two microfluidic techniques allowed us to assess RBC deformability under different shear stresses. Furthermore, we characterized leukocyte stiffness at high shear stresses. The results showed that blood cell deformability–including both RBCs and leukocytes - in general was altered in ChAc patients compared to healthy donors. Therefore, this study shows for the first time an impairment of leukocyte properties in ChAc. During treatment with dasatinib or lithium, we observed alterations in RBC deformability and a stiffness increase for leukocytes. The hematological phenotype of ChAc patients hinted at a reorganization of the cytoskeleton in blood cells which partly explains the altered mechanical properties observed here. These findings highlight the need for a systematic assessment of the contribution of impaired blood cell mechanics to the clinical manifestation of ChAc.

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Section: Discussionmentioning

confidence: 99%

Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium

et al. 2022

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“…Sizes reported in RT-FDC measurements are based on the projected cross-sectional area of the deformed cells. Simulations of the flow fields inside the channel suggest that the stresses near the centerline can be approximated as axisymmetric (40). Therefore, it is fair to assume that the RBCs take up an axisymmetric shape as well and the projected area is directly correlated to cell size.…”

Section: Discussionmentioning

confidence: 99%

Changes in blood cell deformability in Chorea-Acanthocytosis and effects of treatment with dasatinib or lithium

Reichel

Kraeter

Peikert

et al. 2021

Preprint

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Misshaped red blood cells (RBCs), characterized by thorn-like protrusions known as acanthocytes, are a key diagnostic feature in Chorea-Acanthocytosis (ChAc), a rare neurodegenerative disorder. The altered RBC morphology likely influences their biomechanical properties which are crucial for the cells to pass the microvasculature. Here, we investigated blood cell deformability of 5 ChAc patients compared to healthy controls during up to one-year individual off-label treatment with the tyrosine kinases inhibitor dasatinib or several weeks with lithium. Measurements with two microfluidic techniques allowed us to assess RBC deformability under different shear stresses. Furthermore, we characterized leukocyte stiffness at high shear stresses. The results show that blood cell deformability – including both RBCs and leukocytes - in general is altered in ChAc patients compared to healthy donors. Therefore, this study shows for the first time an impairment of leukocyte properties in ChAc. During treatment with dasatinib or lithium, we observe alterations in RBC deformability and a stiffness increase for leukocytes. The hematological phenotype of ChAc patients hints at a reorganization of the cytoskeleton in blood cells which partly explains the altered mechanical properties observed here. These findings highlight the need for a systematic assessment of the contribution of impaired blood cell mechanics to the clinical manifestation of ChAc.

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“…The liquids are stabilised by being pinned either to a surface [66][67][68] or to threads which act as the fluid guides [69]. The free walls are formed from either air [64,65,67,69] or an immiscible fluid [67,68,[70][71][72]. In our system, the two free liquid surfaces are in contact with air as shown in Fig.…”

Section: The Acoustofluidic Capillary Bridgementioning

confidence: 99%

Node formation mechanisms in acoustofluidic capillary bridges

et al. 2022

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Using acoustofluidic channels formed by capillary bridges two models are developed to describe nodes formed by leaky and by evanescent waves. The liquid channel held between a microscope slide (waveguide) and a strip of polystyrene film (fluid guide) avoids solid-sidewall interactions. With this simplification, our experimental and numerical study showed that waves emitted from a single plane surface, interfere and form the nodes without any resonance in the fluid. Both models pay particular attention to tensor elements normal to the solid-liquid interfaces they find that; initially nodes form in the solid and the node pattern is replicated by waves emitted into the fluid from antinodes in the stress. At fluids depths near half an acoustic wavelength, most nodes are formed by leaky waves. In the glass, water-loading reduces node-node separation and forms an overlay type waveguide which aligns the nodes predominantly along the channel. One new practical insight is that node separation can be controlled by water depth. At 0.2 mm water depths (which are smaller than a ¼ wavelength) nodes form from evanescent waves. Here a suspension of yeast cells formed a pattern of small dot-like clumps of cells on the surface of the polystyrene film. We found the same pattern in sound intensity normal, and close, to the waterpolystyrene interface. The capillary bridge channel developed for this study is simple, low-cost, and could be developed for filtration, separation, or patterning of biological species in rapid immuno-sensing applications.

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High-throughput cell and spheroid mechanics in virtual fluidic channels

Cited by 35 publications

References 63 publications

Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium

Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium

Changes in blood cell deformability in Chorea-Acanthocytosis and effects of treatment with dasatinib or lithium

Node formation mechanisms in acoustofluidic capillary bridges

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