High throughput blood plasma separation using a passive PMMA microfluidic device

Shamsi, Alireza; Shamloo, Amir; Mohammadaliha, Negar; Hajghassem, Hassan; Mehrabadi, Jalil Fallah; Bazzaz, Masoumeh

doi:10.1007/s00542-015-2664-7

Cited by 17 publications

(17 citation statements)

References 25 publications

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“…Thermoplastic materials that can be used for low-cost, microfluidic chips are many, mainly polymethyl methacrylate (PMMA), polystyrene (PS), COC, polycarbonate (PC), polyterephthalic acid (PET), polyvinyl chloride (PVC), etc. In thermoplastic plastics, PMMA has been widely used in various life sciences [34] and medical research [35], because of its low cost and good thermal processing and optical properties. PS has excellent biocompatibility, and has significant advantages in the field of cell culture [36] as the matrix material of microfluidic chips; COC is a relatively new amorphous copolymer material.…”

Section: Thermoplasticsmentioning

confidence: 99%

Application of Microfluidic Chip Technology in Food Safety Sensing

Gao

Yan

et al. 2020

Sensors

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Food safety analysis is an important procedure to control food contamination and supervision. It is urgently needed to construct effective methods for on-site, fast, accurate and popular food safety sensing. Among them, microfluidic chip technology exhibits distinguish advantages in detection, including less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability. In this review, we introduce the classification, material, processing and application of the microfluidic chip in food safety sensing, in order to provide a good guide for food safety monitoring.

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Section: Thermoplasticsmentioning

confidence: 99%

Application of Microfluidic Chip Technology in Food Safety Sensing

Gao

Yan

et al. 2020

Sensors

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“…The use of computational methods in collaboration with biological studies in microfluidic devices can improve the understanding of the mechanisms behind certain biological behaviors and also provide ways to enhance the fabrication to achieve optimum designs [14]. Among the applications that computational simulations of microfluidic devices can help are blood-related separation purposes [15,16], cell separation procedures [17,18], and mixing [19,20]. Regarding the numerical simulation of DEP microfluidic devices, Matthew et al [21] conducted a study to investigate the effect of various parameters on the performance of a numerically simulated DEP device.…”

Section: Introductionmentioning

confidence: 99%

Numerical analysis of a dielectrophoresis field‐flow fractionation device for the separation of multiple cell types

Shamloo

Kamali

2017

J of Separation Science

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In this study, a dielectrophoresis field-flow fractionation device was analyzed using a numerical simulation method and the behaviors of a set of different cells were investigated. By reducing the alternating current frequency of the electrodes from the value used in the original setup configuration and increasing the number of exit channels, total discrimination in cell trajectories and subsequent separation of four cell types were achieved. Cells were differentiated based on their size and dielectric response that are represented in their real part of Clausius-Mossotti factor at different frequencies. A number of novel designs were also proposed based on the original setup configuration. It was seen that by reducing the length of the main channel and the number of electrodes at low frequencies and not changing the inlet flow velocities, cell separation was still achieved successfully, although with a slightly larger electrode voltage. The shorter main channel decreased the residence time for the cells on the chip and also reduced the overall size of the device-these were improvements over the original design. The obtained results can be used to analyze other cell types by knowing their size and dielectric properties to design geometries that can ensure separation.

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“…The parameters used in the simulation study are listed in Table I. (14) and (15). At the inlet of the channel, a single blood cell is released from several different vertical positions between 0.4 mm and 0.25 mm, with a horizontal initial velocity.…”

Section: Theoretical Investigation Of Blood Cell Behaviors Undermentioning

confidence: 99%

“…Many different types of microfluidics devices and approaches have been proposed in order to efficiently separate blood cells and blood plasma in blood. [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] One of representative separation methods is to use filtration method. Crowley and Pizziconi 9 demonstrated a microfluidics device which separated blood plasma from whole blood sample using cross filtration method.…”

Section: Introductionmentioning

confidence: 99%

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Low-cost, disposable microfluidics device for blood plasma extraction using continuously alternating paramagnetic and diamagnetic capture modes

Kim

Ong

et al. 2016

Biomicrofluidics

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Blood plasma contains biomarkers and substances that indicate the physiological state of an organism, and it can be used to diagnose various diseases or body condition. To improve the accuracy of diagnostic test, it is required to obtain the high purity of blood plasma. This paper presents a low-cost, disposable microfluidics device for blood plasma extraction using magnetophoretic behaviors of blood cells. This device uses alternating magnetophoretic capture modes to trap and separate paramagnetic and diamagnetic cells away from blood plasma. The device system is composed of two parts, a disposable microfluidics chip and a non-disposable (reusable) magnetic field source. Such modularized device helps the structure of the disposable part dramatically simplified, which is beneficial for low-cost mass production. A series of numerical simulation and parametric study have been performed to describe the mechanism of blood cell separation in the microchannel, and the results are discussed. Furthermore, experimental feasibility test has been carried out in order to demonstrate the blood plasma extraction process of the proposed device. In this experiment, pure blood plasma has been successfully extracted with yield of 21.933% from 75 ll 1:10 dilution of deoxygenated blood.

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High throughput blood plasma separation using a passive PMMA microfluidic device

Cited by 17 publications

References 25 publications

Application of Microfluidic Chip Technology in Food Safety Sensing

Application of Microfluidic Chip Technology in Food Safety Sensing

Numerical analysis of a dielectrophoresis field‐flow fractionation device for the separation of multiple cell types

Low-cost, disposable microfluidics device for blood plasma extraction using continuously alternating paramagnetic and diamagnetic capture modes

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